Cloning and characterization of DELLA genes in Artemisia annua
Gibberellins (GA) are some of the most important phytohormones involved in plant development. DELLA proteins are negative regulators of GA signaling in many plants. In this study, the full-length cDNA sequences of three DELLA genes were cloned from Artemisia annua. Phylogenetic analysis revealed tha...
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| Veröffentlicht in: | Genetics and molecular research 2015-08, Vol.14 (3), p.10037-10049 |
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| creator | Shen, Q Cui, J Fu, X Q Yan, T X Tang, K X |
| description | Gibberellins (GA) are some of the most important phytohormones involved in plant development. DELLA proteins are negative regulators of GA signaling in many plants. In this study, the full-length cDNA sequences of three DELLA genes were cloned from Artemisia annua. Phylogenetic analysis revealed that AaDELLA1 and AaDELLA2 were located in the same cluster, but AaDELLA3 was not. Subcellular localization analysis suggested that AaDELLAs can be targeted to the nucleus and/or cytoplasm. Real-time PCR indicated that all three AaDELLA genes exhibited the highest expression in seeds. Expression of all AaDELLA genes was enhanced by exogenous MeJA treatment but inhibited by GA3 treatment. Yeast two-hybrid assay showed that AaDELLAs could interact with basic helix-loop-helix transcription factor AaMYC2, suggesting that GA and JA signaling may be involved in cross-talk via DELLA and MYC2 interaction in A. annua. |
| doi_str_mv | 10.4238/2015.August.21.10 |
| format | Article |
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DELLA proteins are negative regulators of GA signaling in many plants. In this study, the full-length cDNA sequences of three DELLA genes were cloned from Artemisia annua. Phylogenetic analysis revealed that AaDELLA1 and AaDELLA2 were located in the same cluster, but AaDELLA3 was not. Subcellular localization analysis suggested that AaDELLAs can be targeted to the nucleus and/or cytoplasm. Real-time PCR indicated that all three AaDELLA genes exhibited the highest expression in seeds. Expression of all AaDELLA genes was enhanced by exogenous MeJA treatment but inhibited by GA3 treatment. Yeast two-hybrid assay showed that AaDELLAs could interact with basic helix-loop-helix transcription factor AaMYC2, suggesting that GA and JA signaling may be involved in cross-talk via DELLA and MYC2 interaction in A. annua.</description><identifier>ISSN: 1676-5680</identifier><identifier>EISSN: 1676-5680</identifier><identifier>DOI: 10.4238/2015.August.21.10</identifier><identifier>PMID: 26345940</identifier><language>eng</language><publisher>Brazil</publisher><subject>Amino Acid Sequence ; Artemisia annua ; Artemisia annua - classification ; Artemisia annua - genetics ; Artemisia annua - metabolism ; Cloning, Molecular ; Computational Biology - methods ; DNA, Complementary - chemistry ; DNA, Complementary - genetics ; Evolution, Molecular ; Gene Expression ; Gene Expression Regulation, Plant - drug effects ; Models, Molecular ; Molecular Sequence Data ; Phylogeny ; Plant Growth Regulators - pharmacology ; Plant Proteins - chemistry ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Protein Binding ; Protein Conformation ; Protein Transport ; Recombinant Fusion Proteins - genetics ; Recombinant Fusion Proteins - metabolism ; Sequence Alignment</subject><ispartof>Genetics and molecular research, 2015-08, Vol.14 (3), p.10037-10049</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c377t-75ab2c13fc580395718260b01a052530e4853530fa75441edcf2a4b5db0c90863</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26345940$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shen, Q</creatorcontrib><creatorcontrib>Cui, J</creatorcontrib><creatorcontrib>Fu, X Q</creatorcontrib><creatorcontrib>Yan, T X</creatorcontrib><creatorcontrib>Tang, K X</creatorcontrib><title>Cloning and characterization of DELLA genes in Artemisia annua</title><title>Genetics and molecular research</title><addtitle>Genet Mol Res</addtitle><description>Gibberellins (GA) are some of the most important phytohormones involved in plant development. DELLA proteins are negative regulators of GA signaling in many plants. In this study, the full-length cDNA sequences of three DELLA genes were cloned from Artemisia annua. Phylogenetic analysis revealed that AaDELLA1 and AaDELLA2 were located in the same cluster, but AaDELLA3 was not. Subcellular localization analysis suggested that AaDELLAs can be targeted to the nucleus and/or cytoplasm. Real-time PCR indicated that all three AaDELLA genes exhibited the highest expression in seeds. Expression of all AaDELLA genes was enhanced by exogenous MeJA treatment but inhibited by GA3 treatment. Yeast two-hybrid assay showed that AaDELLAs could interact with basic helix-loop-helix transcription factor AaMYC2, suggesting that GA and JA signaling may be involved in cross-talk via DELLA and MYC2 interaction in A. annua.</description><subject>Amino Acid Sequence</subject><subject>Artemisia annua</subject><subject>Artemisia annua - classification</subject><subject>Artemisia annua - genetics</subject><subject>Artemisia annua - metabolism</subject><subject>Cloning, Molecular</subject><subject>Computational Biology - methods</subject><subject>DNA, Complementary - chemistry</subject><subject>DNA, Complementary - genetics</subject><subject>Evolution, Molecular</subject><subject>Gene Expression</subject><subject>Gene Expression Regulation, Plant - drug effects</subject><subject>Models, Molecular</subject><subject>Molecular Sequence Data</subject><subject>Phylogeny</subject><subject>Plant Growth Regulators - pharmacology</subject><subject>Plant Proteins - chemistry</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Protein Binding</subject><subject>Protein Conformation</subject><subject>Protein Transport</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Recombinant Fusion Proteins - metabolism</subject><subject>Sequence Alignment</subject><issn>1676-5680</issn><issn>1676-5680</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkDtPwzAUhS0EoqXwA1hQRpaE62ecBSkq5SFFYoHZchynGKVOsZMBfj2pWhAj0zm6Oufo6kPoEkPGCJU3BDDPynE9xiEjOMNwhOZY5CLlQsLxHz9DZzG-AxDOJJyiGRGU8YLBHN0uu947v060bxLzpoM2gw3uSw-u90nfJnerqiqTtfU2Js4nZRjsxkWnp4If9Tk6aXUX7cVBF-j1fvWyfEyr54enZVmlhub5kOZc18Rg2hougRY8x5IIqAFr4IRTsExyOmmrc84Yto1piWY1b2owBUhBF-h6v7sN_cdo46CmJ4ztOu1tP0aFc0KwKIik_4hiEJwVsFvF-6gJfYzBtmob3EaHT4VB7QirHWG1J6wIns5T5-owP9Yb2_w2fpDSbxr2dV0</recordid><startdate>20150821</startdate><enddate>20150821</enddate><creator>Shen, Q</creator><creator>Cui, J</creator><creator>Fu, X Q</creator><creator>Yan, T X</creator><creator>Tang, K X</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20150821</creationdate><title>Cloning and characterization of DELLA genes in Artemisia annua</title><author>Shen, Q ; Cui, J ; Fu, X Q ; Yan, T X ; Tang, K X</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c377t-75ab2c13fc580395718260b01a052530e4853530fa75441edcf2a4b5db0c90863</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Amino Acid Sequence</topic><topic>Artemisia annua</topic><topic>Artemisia annua - classification</topic><topic>Artemisia annua - genetics</topic><topic>Artemisia annua - metabolism</topic><topic>Cloning, Molecular</topic><topic>Computational Biology - methods</topic><topic>DNA, Complementary - chemistry</topic><topic>DNA, Complementary - genetics</topic><topic>Evolution, Molecular</topic><topic>Gene Expression</topic><topic>Gene Expression Regulation, Plant - drug effects</topic><topic>Models, Molecular</topic><topic>Molecular Sequence Data</topic><topic>Phylogeny</topic><topic>Plant Growth Regulators - pharmacology</topic><topic>Plant Proteins - chemistry</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Protein Binding</topic><topic>Protein Conformation</topic><topic>Protein Transport</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>Recombinant Fusion Proteins - metabolism</topic><topic>Sequence Alignment</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shen, Q</creatorcontrib><creatorcontrib>Cui, J</creatorcontrib><creatorcontrib>Fu, X Q</creatorcontrib><creatorcontrib>Yan, T X</creatorcontrib><creatorcontrib>Tang, K X</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Genetics and molecular research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shen, Q</au><au>Cui, J</au><au>Fu, X Q</au><au>Yan, T X</au><au>Tang, K X</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning and characterization of DELLA genes in Artemisia annua</atitle><jtitle>Genetics and molecular research</jtitle><addtitle>Genet Mol Res</addtitle><date>2015-08-21</date><risdate>2015</risdate><volume>14</volume><issue>3</issue><spage>10037</spage><epage>10049</epage><pages>10037-10049</pages><issn>1676-5680</issn><eissn>1676-5680</eissn><abstract>Gibberellins (GA) are some of the most important phytohormones involved in plant development. DELLA proteins are negative regulators of GA signaling in many plants. In this study, the full-length cDNA sequences of three DELLA genes were cloned from Artemisia annua. Phylogenetic analysis revealed that AaDELLA1 and AaDELLA2 were located in the same cluster, but AaDELLA3 was not. Subcellular localization analysis suggested that AaDELLAs can be targeted to the nucleus and/or cytoplasm. Real-time PCR indicated that all three AaDELLA genes exhibited the highest expression in seeds. Expression of all AaDELLA genes was enhanced by exogenous MeJA treatment but inhibited by GA3 treatment. Yeast two-hybrid assay showed that AaDELLAs could interact with basic helix-loop-helix transcription factor AaMYC2, suggesting that GA and JA signaling may be involved in cross-talk via DELLA and MYC2 interaction in A. annua.</abstract><cop>Brazil</cop><pmid>26345940</pmid><doi>10.4238/2015.August.21.10</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Amino Acid Sequence Artemisia annua Artemisia annua - classification Artemisia annua - genetics Artemisia annua - metabolism Cloning, Molecular Computational Biology - methods DNA, Complementary - chemistry DNA, Complementary - genetics Evolution, Molecular Gene Expression Gene Expression Regulation, Plant - drug effects Models, Molecular Molecular Sequence Data Phylogeny Plant Growth Regulators - pharmacology Plant Proteins - chemistry Plant Proteins - genetics Plant Proteins - metabolism Protein Binding Protein Conformation Protein Transport Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Sequence Alignment |
| title | Cloning and characterization of DELLA genes in Artemisia annua |
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