A simple, fast, and inexpensive CTAB-PVP-silica based method for genomic DNA isolation from single, small insect larvae and pupae

In this study, we report a modified CTAB-PVP method combined with silicon dioxide (silica) treatment for the extraction of high quality genomic DNA from a single larva or pupa. This method efficiently obtains DNA from small specimens, which is difficult and challenging because of the small amount of...

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Veröffentlicht in:	Genetics and molecular research 2015-07, Vol.14 (3), p.8001-8007
Hauptverfasser:	Huanca-Mamani, W, Rivera-Cabello, D, Maita-Maita, J
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Sequence Cetrimonium Compounds - chemistry Cost-Benefit Analysis DNA - isolation & purification Electron Transport Complex IV - genetics Genome, Insect Insecta - genetics Larva - genetics Molecular Sequence Data Polymerase Chain Reaction - economics Polymerase Chain Reaction - methods Povidone - chemistry Pupa - genetics Sequence Analysis, DNA Silicon Dioxide - chemistry
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