A simple, fast, and inexpensive CTAB-PVP-silica based method for genomic DNA isolation from single, small insect larvae and pupae
In this study, we report a modified CTAB-PVP method combined with silicon dioxide (silica) treatment for the extraction of high quality genomic DNA from a single larva or pupa. This method efficiently obtains DNA from small specimens, which is difficult and challenging because of the small amount of...
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| Veröffentlicht in: | Genetics and molecular research 2015-07, Vol.14 (3), p.8001-8007 |
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| container_title | Genetics and molecular research |
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| creator | Huanca-Mamani, W Rivera-Cabello, D Maita-Maita, J |
| description | In this study, we report a modified CTAB-PVP method combined with silicon dioxide (silica) treatment for the extraction of high quality genomic DNA from a single larva or pupa. This method efficiently obtains DNA from small specimens, which is difficult and challenging because of the small amount of starting tissue. Maceration with liquid nitrogen, phenol treatment, and the ethanol precipitation step are eliminated using this methodology. The A260/A280 absorbance ratios of the isolated DNA were approximately 1.8, suggesting that the DNA is pure and can be used for further molecular analysis. The quality of the isolated DNA permits molecular applications and represents a fast, cheap, and effective alternative method for laboratories with low budgets. |
| doi_str_mv | 10.4238/2015.July.17.8 |
| format | Article |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Animals Base Sequence Cetrimonium Compounds - chemistry Cost-Benefit Analysis DNA - isolation & purification Electron Transport Complex IV - genetics Genome, Insect Insecta - genetics Larva - genetics Molecular Sequence Data Polymerase Chain Reaction - economics Polymerase Chain Reaction - methods Povidone - chemistry Pupa - genetics Sequence Analysis, DNA Silicon Dioxide - chemistry |
| title | A simple, fast, and inexpensive CTAB-PVP-silica based method for genomic DNA isolation from single, small insect larvae and pupae |
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