Molecular analysis and conventional cytology: association between HPV and bacterial vaginosis in the cervical abnormalities of a Brazilian population
We investigated the association between bacterial vaginosis (BV) and human papillomavirus (HPV) infection in Papanicolaou smears in a Brazilian population. Cross-sectional analysis was performed on 673 samples collected from women attending public health centers in Olinda (PE, Brazil) by conventiona...
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| Veröffentlicht in: | Genetics and molecular research 2015-08, Vol.14 (3), p.9497-9505 |
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| creator | Peres, A L Camarotti, J R S L Cartaxo, M Alencar, N Stocco, R C Beçak, W Pontes-Filho, N T Araújo, R F F Lima-Filho, J L Martins, D B G |
| description | We investigated the association between bacterial vaginosis (BV) and human papillomavirus (HPV) infection in Papanicolaou smears in a Brazilian population. Cross-sectional analysis was performed on 673 samples collected from women attending public health centers in Olinda (PE, Brazil) by conventional cytology methodology and molecular analysis, PCR tests (GP5+/6+ and MY09/11). Cytological abnormalities, BV, and HPV-DNA were detected in 23 (3.4%) samples, 189 samples (28.1%), and 210 samples (31.2%), respectively. GP5+/6+ primers resulted in higher detection performance than MY09/11 primers, with 81% concordance between both primers (P < 0.0001). The occurrence of HPV-DNA and BV had ORs of 8.59 (P < 0.0001) and 2.91 (P = 0.0089) for abnormal cytology, respectively, whereas the concomitant presence of both infections showed an OR equal to 3.82 (P = 0.0054). Therefore, we observed an association between abnormal cervical cytology and HPV infection, BV, or both HPV infection and BV. These results highlight the necessity of monitoring patients presenting not only HPV, but also BV, as risk factors for cervical lesion development. |
| doi_str_mv | 10.4238/2015.August.14.13 |
| format | Article |
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Cross-sectional analysis was performed on 673 samples collected from women attending public health centers in Olinda (PE, Brazil) by conventional cytology methodology and molecular analysis, PCR tests (GP5+/6+ and MY09/11). Cytological abnormalities, BV, and HPV-DNA were detected in 23 (3.4%) samples, 189 samples (28.1%), and 210 samples (31.2%), respectively. GP5+/6+ primers resulted in higher detection performance than MY09/11 primers, with 81% concordance between both primers (P < 0.0001). The occurrence of HPV-DNA and BV had ORs of 8.59 (P < 0.0001) and 2.91 (P = 0.0089) for abnormal cytology, respectively, whereas the concomitant presence of both infections showed an OR equal to 3.82 (P = 0.0054). Therefore, we observed an association between abnormal cervical cytology and HPV infection, BV, or both HPV infection and BV. 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Cross-sectional analysis was performed on 673 samples collected from women attending public health centers in Olinda (PE, Brazil) by conventional cytology methodology and molecular analysis, PCR tests (GP5+/6+ and MY09/11). Cytological abnormalities, BV, and HPV-DNA were detected in 23 (3.4%) samples, 189 samples (28.1%), and 210 samples (31.2%), respectively. GP5+/6+ primers resulted in higher detection performance than MY09/11 primers, with 81% concordance between both primers (P < 0.0001). The occurrence of HPV-DNA and BV had ORs of 8.59 (P < 0.0001) and 2.91 (P = 0.0089) for abnormal cytology, respectively, whereas the concomitant presence of both infections showed an OR equal to 3.82 (P = 0.0054). Therefore, we observed an association between abnormal cervical cytology and HPV infection, BV, or both HPV infection and BV. 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Camarotti, J R S L ; Cartaxo, M ; Alencar, N ; Stocco, R C ; Beçak, W ; Pontes-Filho, N T ; Araújo, R F F ; Lima-Filho, J L ; Martins, D B G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c377t-e2167f8717764359cae9951e2a6af0646b7498f9190e1fdfd7e3eb4dfae85d163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adult</topic><topic>Alphapapillomavirus - genetics</topic><topic>Bacteria</topic><topic>Brazil - epidemiology</topic><topic>Cross-Sectional Studies</topic><topic>DNA, Viral</topic><topic>Female</topic><topic>Human papillomavirus</topic><topic>Humans</topic><topic>Middle Aged</topic><topic>Odds Ratio</topic><topic>Papanicolaou Test</topic><topic>Papillomavirus Infections - epidemiology</topic><topic>Papillomavirus Infections - pathology</topic><topic>Papillomavirus Infections - virology</topic><topic>Population Surveillance</topic><topic>Uterine Cervical Neoplasms - epidemiology</topic><topic>Uterine Cervical Neoplasms - etiology</topic><topic>Uterine Cervical Neoplasms - pathology</topic><topic>Vaginosis, Bacterial - epidemiology</topic><topic>Vaginosis, Bacterial - microbiology</topic><topic>Vaginosis, Bacterial - pathology</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Peres, A L</creatorcontrib><creatorcontrib>Camarotti, J R S L</creatorcontrib><creatorcontrib>Cartaxo, M</creatorcontrib><creatorcontrib>Alencar, N</creatorcontrib><creatorcontrib>Stocco, R C</creatorcontrib><creatorcontrib>Beçak, W</creatorcontrib><creatorcontrib>Pontes-Filho, N T</creatorcontrib><creatorcontrib>Araújo, R F F</creatorcontrib><creatorcontrib>Lima-Filho, J L</creatorcontrib><creatorcontrib>Martins, D B G</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Genetics and molecular research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Peres, A L</au><au>Camarotti, J R S L</au><au>Cartaxo, M</au><au>Alencar, N</au><au>Stocco, R C</au><au>Beçak, W</au><au>Pontes-Filho, N T</au><au>Araújo, R F F</au><au>Lima-Filho, J L</au><au>Martins, D B G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular analysis and conventional cytology: association between HPV and bacterial vaginosis in the cervical abnormalities of a Brazilian population</atitle><jtitle>Genetics and molecular research</jtitle><addtitle>Genet Mol Res</addtitle><date>2015-08-14</date><risdate>2015</risdate><volume>14</volume><issue>3</issue><spage>9497</spage><epage>9505</epage><pages>9497-9505</pages><issn>1676-5680</issn><eissn>1676-5680</eissn><abstract>We investigated the association between bacterial vaginosis (BV) and human papillomavirus (HPV) infection in Papanicolaou smears in a Brazilian population. Cross-sectional analysis was performed on 673 samples collected from women attending public health centers in Olinda (PE, Brazil) by conventional cytology methodology and molecular analysis, PCR tests (GP5+/6+ and MY09/11). Cytological abnormalities, BV, and HPV-DNA were detected in 23 (3.4%) samples, 189 samples (28.1%), and 210 samples (31.2%), respectively. GP5+/6+ primers resulted in higher detection performance than MY09/11 primers, with 81% concordance between both primers (P < 0.0001). The occurrence of HPV-DNA and BV had ORs of 8.59 (P < 0.0001) and 2.91 (P = 0.0089) for abnormal cytology, respectively, whereas the concomitant presence of both infections showed an OR equal to 3.82 (P = 0.0054). Therefore, we observed an association between abnormal cervical cytology and HPV infection, BV, or both HPV infection and BV. These results highlight the necessity of monitoring patients presenting not only HPV, but also BV, as risk factors for cervical lesion development.</abstract><cop>Brazil</cop><pmid>26345883</pmid><doi>10.4238/2015.August.14.13</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adult Alphapapillomavirus - genetics Bacteria Brazil - epidemiology Cross-Sectional Studies DNA, Viral Female Human papillomavirus Humans Middle Aged Odds Ratio Papanicolaou Test Papillomavirus Infections - epidemiology Papillomavirus Infections - pathology Papillomavirus Infections - virology Population Surveillance Uterine Cervical Neoplasms - epidemiology Uterine Cervical Neoplasms - etiology Uterine Cervical Neoplasms - pathology Vaginosis, Bacterial - epidemiology Vaginosis, Bacterial - microbiology Vaginosis, Bacterial - pathology Young Adult |
| title | Molecular analysis and conventional cytology: association between HPV and bacterial vaginosis in the cervical abnormalities of a Brazilian population |
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