Blocking the expression of the hepatitis B virus S gene in hepatocellular carcinoma cell lines with an anti-gene locked nucleic acid in vitro
The aim of this study was to investigate the effects of inhibition of the hepatitis B virus (HBV) S gene by polypurine region locked nucleic acid on viral replication in cells. We designed and synthesized a locked nucleic acid, phosphorothioate oligonucleotides, unmodified oligonucleotides, and unre...
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| Veröffentlicht in: | Genetics and molecular research 2015-05, Vol.14 (2), p.5445-5451 |
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| creator | Deng, Y-B Qin, H-J Luo, Y-H Liang, Z-R Zou, J-J |
| description | The aim of this study was to investigate the effects of inhibition of the hepatitis B virus (HBV) S gene by polypurine region locked nucleic acid on viral replication in cells. We designed and synthesized a locked nucleic acid, phosphorothioate oligonucleotides, unmodified oligonucleotides, and unrelated control sequence for the hepatitis B virus S gene polypurine region. HepG2.2.15 cells were transfected by cationic liposome, and fluorescence quantitative polymerase chain reaction technology (PCR) and time-resolved fluoroimmunoassay technology was utilized to monitor the content of HBV DNA, HbsAg, and HBeAg at 2, 4, 6, 8 and 10 days post-transfection. The effects on cell metabolism were detected by four methyl thiazolyl tetrazolium assay. The locked nucleic acid had an obvious effect on HBV DNA replication and HBsAg and HBeAg expression in a dose and time dependent man-ner. The inhibition rates were 52.14, 57.48, and 29.63% after 6 days, respectively. The locked nucleic acid had no significant effect on cell metabolism. The HBV S gene polypurine region locked nucleic acid could effectively inhibit the replication of HBV in vitro, and could provide an effective target for the treatment of HBV and a theoretical and experimental basis for anti-gene therapy. |
| doi_str_mv | 10.4238/2015.May.22.14 |
| format | Article |
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The HBV S gene polypurine region locked nucleic acid could effectively inhibit the replication of HBV in vitro, and could provide an effective target for the treatment of HBV and a theoretical and experimental basis for anti-gene therapy.</description><subject>Carcinoma, Hepatocellular - genetics</subject><subject>Carcinoma, Hepatocellular - pathology</subject><subject>Carcinoma, Hepatocellular - therapy</subject><subject>DNA Replication - drug effects</subject><subject>DNA, Viral - genetics</subject><subject>Gene Expression Regulation, Viral</subject><subject>Genetic Therapy</subject><subject>Hep G2 Cells</subject><subject>Hepatitis B virus</subject><subject>Hepatitis B virus - drug effects</subject><subject>Hepatitis B virus - genetics</subject><subject>Hepatitis B virus - pathogenicity</subject><subject>Humans</subject><subject>Liver Neoplasms - genetics</subject><subject>Liver Neoplasms - pathology</subject><subject>Liver Neoplasms - therapy</subject><subject>Oligonucleotides - administration & dosage</subject><subject>Oligonucleotides - genetics</subject><subject>Viral Envelope Proteins - antagonists & inhibitors</subject><subject>Viral Envelope Proteins - biosynthesis</subject><subject>Viral Envelope Proteins - genetics</subject><issn>1676-5680</issn><issn>1676-5680</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUbtu3DAQJAwHfrcpA5ZuJHNJ8aHSNvwCbKRIUhM8auVjoiPPpOTEH5F_9snnBOkCLLCL2ZnZBYaQj8DqhgtzxhnI-sG91JzX0OyQA1BaVVIZtvvPvE8OS_nOGJeNYXtknyvgUjfsgPy-GJL_EeIjHZdI8dc6YykhRZr6N2SJazeGMRR6QZ9Dngr9Qh8xIg1xu0seh2EaXKbeZR9iWjk6Q3QIEQv9GcYldXFTY6jehPM97Gic_IDBU-dDN5s9hzGnY_Khd0PBk_d-RL5dX329vK3uP9_cXZ7fV14oM1atUFyrvgfdGy8Y85xJAQsDrXLYC8eMbFFybcCYbqGwX3RMQst9ywUoBuKInG591zk9TVhGuwpl_tpFTFOxoDkHJaVu_09VrQCjG6E31HpL9TmVkrG36xxWLr9YYHZOy85p2U1alnMLzUbw6d17Wqyw-0v_E494BQoJkFc</recordid><startdate>20150522</startdate><enddate>20150522</enddate><creator>Deng, Y-B</creator><creator>Qin, H-J</creator><creator>Luo, Y-H</creator><creator>Liang, Z-R</creator><creator>Zou, J-J</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20150522</creationdate><title>Blocking the expression of the hepatitis B virus S gene in hepatocellular carcinoma cell lines with an anti-gene locked nucleic acid in vitro</title><author>Deng, Y-B ; Qin, H-J ; Luo, Y-H ; Liang, Z-R ; Zou, J-J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c368t-936276ff17f8c300c20531b8196aef3a0859e5278188db6efbd05192c92316013</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Carcinoma, Hepatocellular - genetics</topic><topic>Carcinoma, Hepatocellular - pathology</topic><topic>Carcinoma, Hepatocellular - therapy</topic><topic>DNA Replication - drug effects</topic><topic>DNA, Viral - genetics</topic><topic>Gene Expression Regulation, Viral</topic><topic>Genetic Therapy</topic><topic>Hep G2 Cells</topic><topic>Hepatitis B virus</topic><topic>Hepatitis B virus - drug effects</topic><topic>Hepatitis B virus - genetics</topic><topic>Hepatitis B virus - pathogenicity</topic><topic>Humans</topic><topic>Liver Neoplasms - genetics</topic><topic>Liver Neoplasms - pathology</topic><topic>Liver Neoplasms - therapy</topic><topic>Oligonucleotides - administration & dosage</topic><topic>Oligonucleotides - genetics</topic><topic>Viral Envelope Proteins - antagonists & inhibitors</topic><topic>Viral Envelope Proteins - biosynthesis</topic><topic>Viral Envelope Proteins - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Deng, Y-B</creatorcontrib><creatorcontrib>Qin, H-J</creatorcontrib><creatorcontrib>Luo, Y-H</creatorcontrib><creatorcontrib>Liang, Z-R</creatorcontrib><creatorcontrib>Zou, J-J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Genetics and molecular research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Deng, Y-B</au><au>Qin, H-J</au><au>Luo, Y-H</au><au>Liang, Z-R</au><au>Zou, J-J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Blocking the expression of the hepatitis B virus S gene in hepatocellular carcinoma cell lines with an anti-gene locked nucleic acid in vitro</atitle><jtitle>Genetics and molecular research</jtitle><addtitle>Genet Mol Res</addtitle><date>2015-05-22</date><risdate>2015</risdate><volume>14</volume><issue>2</issue><spage>5445</spage><epage>5451</epage><pages>5445-5451</pages><issn>1676-5680</issn><eissn>1676-5680</eissn><abstract>The aim of this study was to investigate the effects of inhibition of the hepatitis B virus (HBV) S gene by polypurine region locked nucleic acid on viral replication in cells. We designed and synthesized a locked nucleic acid, phosphorothioate oligonucleotides, unmodified oligonucleotides, and unrelated control sequence for the hepatitis B virus S gene polypurine region. HepG2.2.15 cells were transfected by cationic liposome, and fluorescence quantitative polymerase chain reaction technology (PCR) and time-resolved fluoroimmunoassay technology was utilized to monitor the content of HBV DNA, HbsAg, and HBeAg at 2, 4, 6, 8 and 10 days post-transfection. The effects on cell metabolism were detected by four methyl thiazolyl tetrazolium assay. The locked nucleic acid had an obvious effect on HBV DNA replication and HBsAg and HBeAg expression in a dose and time dependent man-ner. The inhibition rates were 52.14, 57.48, and 29.63% after 6 days, respectively. The locked nucleic acid had no significant effect on cell metabolism. The HBV S gene polypurine region locked nucleic acid could effectively inhibit the replication of HBV in vitro, and could provide an effective target for the treatment of HBV and a theoretical and experimental basis for anti-gene therapy.</abstract><cop>Brazil</cop><pmid>26125740</pmid><doi>10.4238/2015.May.22.14</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Carcinoma, Hepatocellular - genetics Carcinoma, Hepatocellular - pathology Carcinoma, Hepatocellular - therapy DNA Replication - drug effects DNA, Viral - genetics Gene Expression Regulation, Viral Genetic Therapy Hep G2 Cells Hepatitis B virus Hepatitis B virus - drug effects Hepatitis B virus - genetics Hepatitis B virus - pathogenicity Humans Liver Neoplasms - genetics Liver Neoplasms - pathology Liver Neoplasms - therapy Oligonucleotides - administration & dosage Oligonucleotides - genetics Viral Envelope Proteins - antagonists & inhibitors Viral Envelope Proteins - biosynthesis Viral Envelope Proteins - genetics |
| title | Blocking the expression of the hepatitis B virus S gene in hepatocellular carcinoma cell lines with an anti-gene locked nucleic acid in vitro |
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