Screening and characterization of novel polymorphic microsatellite markers from sea cucumber Holothuria leucospilota
The sea cucumber Holothuria leucospilota has high me-dicinal value and rich nutritional edible value, and thus is a commercially important aquatic product in China. Microsatellite loci were developed and screened using a fast isolation protocol and amplified fragment length polymorphism of sequences...
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| Veröffentlicht in: | Genetics and molecular research 2015-06, Vol.14 (2), p.6555-6560 |
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| creator | Shangguan, J B Li, Z B Ning, Y F Huang, Y S Yuan, Y Lu, J Li, B B Mao, X Q |
| description | The sea cucumber Holothuria leucospilota has high me-dicinal value and rich nutritional edible value, and thus is a commercially important aquatic product in China. Microsatellite loci were developed and screened using a fast isolation protocol and amplified fragment length polymorphism of sequences containing repeats. In this study, 16 novel polymorphic microsatellite markers in H. leucospilota were identified, and the relevant genetic variability index was assessed using 30 individu-als from a wild population. The polymorphic information content ranged from 0.183 to 0.668, and the number of alleles per locus varied from 3 to 5. The observed and expected heterozygosities were 0.0370-0.5000 and 0.0776-0.6250, respectively. With the exception of 3 loci (Y1-15, Y11-1, and Y28), the polymorphic loci were in Hardy-Weinberg equilibrium (P > 0.003125). These polymorphic microsatellite loci will contribute to studies of genetic diversity, the research of population structure, and the design of conservation strategies for H. leucospilota. |
| doi_str_mv | 10.4238/2015.June.12.9 |
| format | Article |
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Microsatellite loci were developed and screened using a fast isolation protocol and amplified fragment length polymorphism of sequences containing repeats. In this study, 16 novel polymorphic microsatellite markers in H. leucospilota were identified, and the relevant genetic variability index was assessed using 30 individu-als from a wild population. The polymorphic information content ranged from 0.183 to 0.668, and the number of alleles per locus varied from 3 to 5. The observed and expected heterozygosities were 0.0370-0.5000 and 0.0776-0.6250, respectively. With the exception of 3 loci (Y1-15, Y11-1, and Y28), the polymorphic loci were in Hardy-Weinberg equilibrium (P > 0.003125). These polymorphic microsatellite loci will contribute to studies of genetic diversity, the research of population structure, and the design of conservation strategies for H. leucospilota.</description><identifier>ISSN: 1676-5680</identifier><identifier>EISSN: 1676-5680</identifier><identifier>DOI: 10.4238/2015.June.12.9</identifier><identifier>PMID: 26125861</identifier><language>eng</language><publisher>Brazil</publisher><subject>Amplified Fragment Length Polymorphism Analysis ; Animals ; Genetic Variation ; Heterozygote ; Holothuria leucospilota ; Holothurioidea ; Microsatellite Repeats - genetics ; Polymorphism, Genetic ; Sea Cucumbers - genetics</subject><ispartof>Genetics and molecular research, 2015-06, Vol.14 (2), p.6555-6560</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c368t-142de868d501ee3b07d6598420caf447cc1a83225732bc0c36ead5770f46bd5b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26125861$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shangguan, J B</creatorcontrib><creatorcontrib>Li, Z B</creatorcontrib><creatorcontrib>Ning, Y F</creatorcontrib><creatorcontrib>Huang, Y S</creatorcontrib><creatorcontrib>Yuan, Y</creatorcontrib><creatorcontrib>Lu, J</creatorcontrib><creatorcontrib>Li, B B</creatorcontrib><creatorcontrib>Mao, X Q</creatorcontrib><title>Screening and characterization of novel polymorphic microsatellite markers from sea cucumber Holothuria leucospilota</title><title>Genetics and molecular research</title><addtitle>Genet Mol Res</addtitle><description>The sea cucumber Holothuria leucospilota has high me-dicinal value and rich nutritional edible value, and thus is a commercially important aquatic product in China. Microsatellite loci were developed and screened using a fast isolation protocol and amplified fragment length polymorphism of sequences containing repeats. In this study, 16 novel polymorphic microsatellite markers in H. leucospilota were identified, and the relevant genetic variability index was assessed using 30 individu-als from a wild population. The polymorphic information content ranged from 0.183 to 0.668, and the number of alleles per locus varied from 3 to 5. The observed and expected heterozygosities were 0.0370-0.5000 and 0.0776-0.6250, respectively. With the exception of 3 loci (Y1-15, Y11-1, and Y28), the polymorphic loci were in Hardy-Weinberg equilibrium (P > 0.003125). These polymorphic microsatellite loci will contribute to studies of genetic diversity, the research of population structure, and the design of conservation strategies for H. leucospilota.</description><subject>Amplified Fragment Length Polymorphism Analysis</subject><subject>Animals</subject><subject>Genetic Variation</subject><subject>Heterozygote</subject><subject>Holothuria leucospilota</subject><subject>Holothurioidea</subject><subject>Microsatellite Repeats - genetics</subject><subject>Polymorphism, Genetic</subject><subject>Sea Cucumbers - genetics</subject><issn>1676-5680</issn><issn>1676-5680</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkb1PwzAQxS0EoqWwMiKPLA3-iD86ogooCIkBmC3HuVBDEgc7QSp_PakoiI3pTqf3nk7vh9ApJVnOuL5ghIrsbmghoyxb7KEplUrOhdRk_88-QUcpvRLCRK7JIZowSZnQkk5R_-giQOvbF2zbEru1jdb1EP2n7X1ocahwGz6gxl2oN02I3do73HgXQ7I91LXvATc2vkFMuIqhwQksdoMbmgIiXoU69OsheotrGFxInR8P9hgdVLZOcLKbM_R8ffW0XM3vH25ul5f3c8el7uc0ZyVoqUtBKAAviCqlWOicEWerPFfOUas5Y0JxVjgymsCWQilS5bIoRcFn6Pw7t4vhfYDUm8YnN35tWwhDMlQxRmVONPtfKhecaqUkH6XZt3RbQopQmS76sYONocRsoZgtFLOFYigzi9FwtsseigbKX_kPBf4FLNGKvQ</recordid><startdate>20150612</startdate><enddate>20150612</enddate><creator>Shangguan, J B</creator><creator>Li, Z B</creator><creator>Ning, Y F</creator><creator>Huang, Y S</creator><creator>Yuan, Y</creator><creator>Lu, J</creator><creator>Li, B B</creator><creator>Mao, X Q</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20150612</creationdate><title>Screening and characterization of novel polymorphic microsatellite markers from sea cucumber Holothuria leucospilota</title><author>Shangguan, J B ; Li, Z B ; Ning, Y F ; Huang, Y S ; Yuan, Y ; Lu, J ; Li, B B ; Mao, X Q</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c368t-142de868d501ee3b07d6598420caf447cc1a83225732bc0c36ead5770f46bd5b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Amplified Fragment Length Polymorphism Analysis</topic><topic>Animals</topic><topic>Genetic Variation</topic><topic>Heterozygote</topic><topic>Holothuria leucospilota</topic><topic>Holothurioidea</topic><topic>Microsatellite Repeats - genetics</topic><topic>Polymorphism, Genetic</topic><topic>Sea Cucumbers - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shangguan, J B</creatorcontrib><creatorcontrib>Li, Z B</creatorcontrib><creatorcontrib>Ning, Y F</creatorcontrib><creatorcontrib>Huang, Y S</creatorcontrib><creatorcontrib>Yuan, Y</creatorcontrib><creatorcontrib>Lu, J</creatorcontrib><creatorcontrib>Li, B B</creatorcontrib><creatorcontrib>Mao, X Q</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Genetics and molecular research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shangguan, J B</au><au>Li, Z B</au><au>Ning, Y F</au><au>Huang, Y S</au><au>Yuan, Y</au><au>Lu, J</au><au>Li, B B</au><au>Mao, X Q</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Screening and characterization of novel polymorphic microsatellite markers from sea cucumber Holothuria leucospilota</atitle><jtitle>Genetics and molecular research</jtitle><addtitle>Genet Mol Res</addtitle><date>2015-06-12</date><risdate>2015</risdate><volume>14</volume><issue>2</issue><spage>6555</spage><epage>6560</epage><pages>6555-6560</pages><issn>1676-5680</issn><eissn>1676-5680</eissn><abstract>The sea cucumber Holothuria leucospilota has high me-dicinal value and rich nutritional edible value, and thus is a commercially important aquatic product in China. Microsatellite loci were developed and screened using a fast isolation protocol and amplified fragment length polymorphism of sequences containing repeats. In this study, 16 novel polymorphic microsatellite markers in H. leucospilota were identified, and the relevant genetic variability index was assessed using 30 individu-als from a wild population. The polymorphic information content ranged from 0.183 to 0.668, and the number of alleles per locus varied from 3 to 5. The observed and expected heterozygosities were 0.0370-0.5000 and 0.0776-0.6250, respectively. With the exception of 3 loci (Y1-15, Y11-1, and Y28), the polymorphic loci were in Hardy-Weinberg equilibrium (P > 0.003125). These polymorphic microsatellite loci will contribute to studies of genetic diversity, the research of population structure, and the design of conservation strategies for H. leucospilota.</abstract><cop>Brazil</cop><pmid>26125861</pmid><doi>10.4238/2015.June.12.9</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Amplified Fragment Length Polymorphism Analysis Animals Genetic Variation Heterozygote Holothuria leucospilota Holothurioidea Microsatellite Repeats - genetics Polymorphism, Genetic Sea Cucumbers - genetics |
| title | Screening and characterization of novel polymorphic microsatellite markers from sea cucumber Holothuria leucospilota |
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