Pleural fluid cell-free DNA in parapneumonic pleural effusion
To measure the accuracy of pleural fluid cell-free DNA (cfDNA) concentration for diagnosis of parapneumonic pleural effusions (PPE). We studied pleural fluids obtained by thoracocentesis in patients with pleural effusion. DNA was automatically extracted from pleural fluid using the MagNa Pure Compac...
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| Veröffentlicht in: | Clinical biochemistry 2015-10, Vol.48 (15), p.1003-1005 |
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| creator | Santotoribio, Jose D. Cabrera-Alarcón, Jose L. Batalha-Caetano, Paula Macher, Hada C. Guerrero, Juan M. |
| description | To measure the accuracy of pleural fluid cell-free DNA (cfDNA) concentration for diagnosis of parapneumonic pleural effusions (PPE).
We studied pleural fluids obtained by thoracocentesis in patients with pleural effusion. DNA was automatically extracted from pleural fluid using the MagNa Pure Compact instrument (Roche Diagnostics), and was measured by a real-time quantitative PCR assay for the β-globin gene using a Light-Cycler 480 Real-Time PCR instrument (Roche Diagnostics). Patients were classified into two groups according to the etiology of pleural effusion: PPE and NOT PPE. The diagnostic accuracy was determined using receiver operating characteristic (ROC) techniques by analyzing the area under the ROC curve (AUC).
We studied 78 patients with ages between 1 and 86years old (median=64). Sixteen patients were PPE and 62 were NOT PPE (24 transudative, 30 malignant and 8 other etiology). Pleural fluid cfDNA concentration was higher in patients with PPE (median=46,240ng/mL) than in those with NOT PPE (median=224ng/mL). The AUC value was 0.907 (p |
| doi_str_mv | 10.1016/j.clinbiochem.2015.07.096 |
| format | Article |
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We studied pleural fluids obtained by thoracocentesis in patients with pleural effusion. DNA was automatically extracted from pleural fluid using the MagNa Pure Compact instrument (Roche Diagnostics), and was measured by a real-time quantitative PCR assay for the β-globin gene using a Light-Cycler 480 Real-Time PCR instrument (Roche Diagnostics). Patients were classified into two groups according to the etiology of pleural effusion: PPE and NOT PPE. The diagnostic accuracy was determined using receiver operating characteristic (ROC) techniques by analyzing the area under the ROC curve (AUC).
We studied 78 patients with ages between 1 and 86years old (median=64). Sixteen patients were PPE and 62 were NOT PPE (24 transudative, 30 malignant and 8 other etiology). Pleural fluid cfDNA concentration was higher in patients with PPE (median=46,240ng/mL) than in those with NOT PPE (median=224ng/mL). The AUC value was 0.907 (p<0.0001) and the optimal cut-off value was 6740ng/mL exhibiting 87.5% sensitivity and 80.6% specificity. Also, there were significant differences between transudative and exudative effusions according to pleural fluid cfDNA concentration (p<0.0001). The AUC value was 0.994 and the optimal cut-off value was 162ng/mL exhibiting 100% sensitivity and 96.3% specificity.
Pleural fluid cfDNA concentration showed high accuracy for diagnosis of PPE and to discriminate between transudative and exudative effusions.
•There is an association between cfDNA and local inflammation in the pleura.•cfDNA has higher accuracy for diagnosis of PPE when compared with LDH or glucose.•cfDNA shows high accuracy to predict whether a PPE is uncomplicated or complicated.•cfDNA could be a potential new tool for diagnosis and management of PPE.</description><identifier>ISSN: 0009-9120</identifier><identifier>EISSN: 1873-2933</identifier><identifier>DOI: 10.1016/j.clinbiochem.2015.07.096</identifier><identifier>PMID: 26234638</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adolescent ; Adult ; Aged ; Biomarkers - metabolism ; Cell-free DNA ; Child ; Diagnosis, Differential ; DNA - metabolism ; Exudates and Transudates - chemistry ; Female ; Humans ; Infant ; Male ; Molecular Diagnostic Techniques ; Parapneumonic effusion ; Pleural effusion ; Pleural Effusion - diagnosis ; Pleural Effusion - etiology ; Pleural Effusion - metabolism ; Pleural Effusion, Malignant - diagnosis ; Pleural Effusion, Malignant - etiology ; Pleural Effusion, Malignant - metabolism ; Pleural fluid ; Pneumonia ; Pneumonia - physiopathology ; Real-Time Polymerase Chain Reaction ; Sensitivity and Specificity ; Thoracentesis ; Up-Regulation</subject><ispartof>Clinical biochemistry, 2015-10, Vol.48 (15), p.1003-1005</ispartof><rights>2015</rights><rights>Copyright © 2015. Published by Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c447t-385cc468544a6ca8a3cd5934c5ec9cf93de48554c5134ca47d81f7f48b7b34e53</citedby><cites>FETCH-LOGICAL-c447t-385cc468544a6ca8a3cd5934c5ec9cf93de48554c5134ca47d81f7f48b7b34e53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.clinbiochem.2015.07.096$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27907,27908,45978</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26234638$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Santotoribio, Jose D.</creatorcontrib><creatorcontrib>Cabrera-Alarcón, Jose L.</creatorcontrib><creatorcontrib>Batalha-Caetano, Paula</creatorcontrib><creatorcontrib>Macher, Hada C.</creatorcontrib><creatorcontrib>Guerrero, Juan M.</creatorcontrib><title>Pleural fluid cell-free DNA in parapneumonic pleural effusion</title><title>Clinical biochemistry</title><addtitle>Clin Biochem</addtitle><description>To measure the accuracy of pleural fluid cell-free DNA (cfDNA) concentration for diagnosis of parapneumonic pleural effusions (PPE).
We studied pleural fluids obtained by thoracocentesis in patients with pleural effusion. DNA was automatically extracted from pleural fluid using the MagNa Pure Compact instrument (Roche Diagnostics), and was measured by a real-time quantitative PCR assay for the β-globin gene using a Light-Cycler 480 Real-Time PCR instrument (Roche Diagnostics). Patients were classified into two groups according to the etiology of pleural effusion: PPE and NOT PPE. The diagnostic accuracy was determined using receiver operating characteristic (ROC) techniques by analyzing the area under the ROC curve (AUC).
We studied 78 patients with ages between 1 and 86years old (median=64). Sixteen patients were PPE and 62 were NOT PPE (24 transudative, 30 malignant and 8 other etiology). Pleural fluid cfDNA concentration was higher in patients with PPE (median=46,240ng/mL) than in those with NOT PPE (median=224ng/mL). The AUC value was 0.907 (p<0.0001) and the optimal cut-off value was 6740ng/mL exhibiting 87.5% sensitivity and 80.6% specificity. Also, there were significant differences between transudative and exudative effusions according to pleural fluid cfDNA concentration (p<0.0001). The AUC value was 0.994 and the optimal cut-off value was 162ng/mL exhibiting 100% sensitivity and 96.3% specificity.
Pleural fluid cfDNA concentration showed high accuracy for diagnosis of PPE and to discriminate between transudative and exudative effusions.
•There is an association between cfDNA and local inflammation in the pleura.•cfDNA has higher accuracy for diagnosis of PPE when compared with LDH or glucose.•cfDNA shows high accuracy to predict whether a PPE is uncomplicated or complicated.•cfDNA could be a potential new tool for diagnosis and management of PPE.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Biomarkers - metabolism</subject><subject>Cell-free DNA</subject><subject>Child</subject><subject>Diagnosis, Differential</subject><subject>DNA - metabolism</subject><subject>Exudates and Transudates - chemistry</subject><subject>Female</subject><subject>Humans</subject><subject>Infant</subject><subject>Male</subject><subject>Molecular Diagnostic Techniques</subject><subject>Parapneumonic effusion</subject><subject>Pleural effusion</subject><subject>Pleural Effusion - diagnosis</subject><subject>Pleural Effusion - etiology</subject><subject>Pleural Effusion - metabolism</subject><subject>Pleural Effusion, Malignant - diagnosis</subject><subject>Pleural Effusion, Malignant - etiology</subject><subject>Pleural Effusion, Malignant - metabolism</subject><subject>Pleural fluid</subject><subject>Pneumonia</subject><subject>Pneumonia - physiopathology</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Sensitivity and Specificity</subject><subject>Thoracentesis</subject><subject>Up-Regulation</subject><issn>0009-9120</issn><issn>1873-2933</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkLtOAzEQRS0EIiHwC2jpaHbxc20XFFF4ShFQQG053rFwtC_sLBJ_z0YJiJJqNKM7c-cehC4ILggm5dW6cHVoV6Fz79AUFBNRYFlgXR6gKVGS5VQzdoimGGOda0LxBJ2ktB5bylV5jCa0pIyXTE3R9UsNQ7R15ushVJmDus59BMhunuZZaLPeRtu3MDRdG1zW78Xg_ZBC156iI2_rBGf7OkNvd7evi4d8-Xz_uJgvc8e53ORMCed4qQTntnRWWeYqoRl3Apx2XrMKuBJi7Mk4tFxWinjpuVrJFeMg2Axd7u72sfsYIG1ME9L2V9tCNyRDJCUlo2qMNUN6J3WxSymCN30MjY1fhmCzpWfW5g89s6VnsDQjvXH3fG8zrBqofjd_cI2CxU4AY9jPANEkF6B1UIUIbmOqLvzD5htYZYYL</recordid><startdate>20151001</startdate><enddate>20151001</enddate><creator>Santotoribio, Jose D.</creator><creator>Cabrera-Alarcón, Jose L.</creator><creator>Batalha-Caetano, Paula</creator><creator>Macher, Hada C.</creator><creator>Guerrero, Juan M.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20151001</creationdate><title>Pleural fluid cell-free DNA in parapneumonic pleural effusion</title><author>Santotoribio, Jose D. ; Cabrera-Alarcón, Jose L. ; Batalha-Caetano, Paula ; Macher, Hada C. ; Guerrero, Juan M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c447t-385cc468544a6ca8a3cd5934c5ec9cf93de48554c5134ca47d81f7f48b7b34e53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Biomarkers - metabolism</topic><topic>Cell-free DNA</topic><topic>Child</topic><topic>Diagnosis, Differential</topic><topic>DNA - metabolism</topic><topic>Exudates and Transudates - chemistry</topic><topic>Female</topic><topic>Humans</topic><topic>Infant</topic><topic>Male</topic><topic>Molecular Diagnostic Techniques</topic><topic>Parapneumonic effusion</topic><topic>Pleural effusion</topic><topic>Pleural Effusion - diagnosis</topic><topic>Pleural Effusion - etiology</topic><topic>Pleural Effusion - metabolism</topic><topic>Pleural Effusion, Malignant - diagnosis</topic><topic>Pleural Effusion, Malignant - etiology</topic><topic>Pleural Effusion, Malignant - metabolism</topic><topic>Pleural fluid</topic><topic>Pneumonia</topic><topic>Pneumonia - physiopathology</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Sensitivity and Specificity</topic><topic>Thoracentesis</topic><topic>Up-Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Santotoribio, Jose D.</creatorcontrib><creatorcontrib>Cabrera-Alarcón, Jose L.</creatorcontrib><creatorcontrib>Batalha-Caetano, Paula</creatorcontrib><creatorcontrib>Macher, Hada C.</creatorcontrib><creatorcontrib>Guerrero, Juan M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Santotoribio, Jose D.</au><au>Cabrera-Alarcón, Jose L.</au><au>Batalha-Caetano, Paula</au><au>Macher, Hada C.</au><au>Guerrero, Juan M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pleural fluid cell-free DNA in parapneumonic pleural effusion</atitle><jtitle>Clinical biochemistry</jtitle><addtitle>Clin Biochem</addtitle><date>2015-10-01</date><risdate>2015</risdate><volume>48</volume><issue>15</issue><spage>1003</spage><epage>1005</epage><pages>1003-1005</pages><issn>0009-9120</issn><eissn>1873-2933</eissn><abstract>To measure the accuracy of pleural fluid cell-free DNA (cfDNA) concentration for diagnosis of parapneumonic pleural effusions (PPE).
We studied pleural fluids obtained by thoracocentesis in patients with pleural effusion. DNA was automatically extracted from pleural fluid using the MagNa Pure Compact instrument (Roche Diagnostics), and was measured by a real-time quantitative PCR assay for the β-globin gene using a Light-Cycler 480 Real-Time PCR instrument (Roche Diagnostics). Patients were classified into two groups according to the etiology of pleural effusion: PPE and NOT PPE. The diagnostic accuracy was determined using receiver operating characteristic (ROC) techniques by analyzing the area under the ROC curve (AUC).
We studied 78 patients with ages between 1 and 86years old (median=64). Sixteen patients were PPE and 62 were NOT PPE (24 transudative, 30 malignant and 8 other etiology). Pleural fluid cfDNA concentration was higher in patients with PPE (median=46,240ng/mL) than in those with NOT PPE (median=224ng/mL). The AUC value was 0.907 (p<0.0001) and the optimal cut-off value was 6740ng/mL exhibiting 87.5% sensitivity and 80.6% specificity. Also, there were significant differences between transudative and exudative effusions according to pleural fluid cfDNA concentration (p<0.0001). The AUC value was 0.994 and the optimal cut-off value was 162ng/mL exhibiting 100% sensitivity and 96.3% specificity.
Pleural fluid cfDNA concentration showed high accuracy for diagnosis of PPE and to discriminate between transudative and exudative effusions.
•There is an association between cfDNA and local inflammation in the pleura.•cfDNA has higher accuracy for diagnosis of PPE when compared with LDH or glucose.•cfDNA shows high accuracy to predict whether a PPE is uncomplicated or complicated.•cfDNA could be a potential new tool for diagnosis and management of PPE.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>26234638</pmid><doi>10.1016/j.clinbiochem.2015.07.096</doi><tpages>3</tpages></addata></record> |
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| subjects | Adolescent Adult Aged Biomarkers - metabolism Cell-free DNA Child Diagnosis, Differential DNA - metabolism Exudates and Transudates - chemistry Female Humans Infant Male Molecular Diagnostic Techniques Parapneumonic effusion Pleural effusion Pleural Effusion - diagnosis Pleural Effusion - etiology Pleural Effusion - metabolism Pleural Effusion, Malignant - diagnosis Pleural Effusion, Malignant - etiology Pleural Effusion, Malignant - metabolism Pleural fluid Pneumonia Pneumonia - physiopathology Real-Time Polymerase Chain Reaction Sensitivity and Specificity Thoracentesis Up-Regulation |
| title | Pleural fluid cell-free DNA in parapneumonic pleural effusion |
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