A single nanoparticle-based sensor for hydrogen peroxide (H2O2) via cytochrome c-mediated plasmon resonance energy transfer

Herein, we report a novel method for H2O2 detection based on a single plasmonic nanoprobe via cytochrome c (Cyt c)-mediated plasmon resonance energy transfer (PRET). Dynamic spectral changes were observed in the fingerprint quenching dip of a single plasmonic nanoprobe in response to changes in the...

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Veröffentlicht in:Chemical communications (Cambridge, England) England), 2015-01, Vol.51 (84), p.15370-15373
Hauptverfasser: Kim, Yura, Park, Ji Youn, Kim, Hye Young, Lee, Minzae, Yi, Jongheop, Choi, Inhee
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container_issue 84
container_start_page 15370
container_title Chemical communications (Cambridge, England)
container_volume 51
creator Kim, Yura
Park, Ji Youn
Kim, Hye Young
Lee, Minzae
Yi, Jongheop
Choi, Inhee
description Herein, we report a novel method for H2O2 detection based on a single plasmonic nanoprobe via cytochrome c (Cyt c)-mediated plasmon resonance energy transfer (PRET). Dynamic spectral changes were observed in the fingerprint quenching dip of a single plasmonic nanoprobe in response to changes in the redox state of Cyt c, induced by H2O2. Based on the changes in the spectral profile of the single plasmonic nanoprobe, H2O2 was successfully detected in a wide concentration range from 100 mM to 10 nM, including physiologically relevant micromolar and nanomolar concentrations.
doi_str_mv 10.1039/c5cc05327g
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source MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection
subjects Cytochromes c - chemistry
Cytochromes c - metabolism
Fluorescence Resonance Energy Transfer
Gold - chemistry
Hydrogen Peroxide - analysis
Hydrogen Peroxide - metabolism
Metal Nanoparticles - chemistry
Oxidation-Reduction
title A single nanoparticle-based sensor for hydrogen peroxide (H2O2) via cytochrome c-mediated plasmon resonance energy transfer
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