Expression of the insecticidal lectin from snowdrop (Galanthus nivalis agglutinin; GNA) in transgenic wheat plants: effects on predation by the grain aphid Sitobion avenae
Transgenic wheat plants containing the gene encoding snowdrop lectin (Galanthus nivalis agglutinin; GNA) under the control of constitutive and phloem-specific promoters were generated through the particle bombardment method. Thirty-two independently derived plants were subjected to molecular and bio...
Gespeichert in:
| Veröffentlicht in: | Molecular breeding 1999, Vol.5 (1), p.65-73 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 73 |
|---|---|
| container_issue | 1 |
| container_start_page | 65 |
| container_title | Molecular breeding |
| container_volume | 5 |
| creator | Stoger, E. (John Innes Centre, Norwich (United Kingdom).) Williams, S Christou, P Down, R.E Gatehouse, J.A |
| description | Transgenic wheat plants containing the gene encoding snowdrop lectin (Galanthus nivalis agglutinin; GNA) under the control of constitutive and phloem-specific promoters were generated through the particle bombardment method. Thirty-two independently derived plants were subjected to molecular and biochemical analyses. Transgene integration varied from one to twelve estimated copies per haploid genome, and levels of GNA expression from 0 to ca. 0.2% of total soluble protein were observed in different transgenic plants. Seven transgenic plants were selected for further study. Progeny plants from these parental transformants were selected for transgene expression, and tested for enhanced resistance to the grain aphid (Sitobion avenae) by exposing the plants to nymphal insects under glasshouse conditions. Bioassay results show that transgenic wheat plants from lines expressing GNA at levels greater than ca. 0.04% of total soluble protein decrease the fecundity, but not the survival, of grain aphids. We propose that transgenic approaches using insecticidal genes such as gna in combination with integrated pest management present promising opportunities for the control of damaging wheat pests. |
| doi_str_mv | 10.1023/A:1009616413886 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_fao_a</sourceid><recordid>TN_cdi_proquest_miscellaneous_17206802</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>17206802</sourcerecordid><originalsourceid>FETCH-LOGICAL-c258t-597ba4ef28bbca0ced4a971355ad32d26fbe95b9d44f079bfcbc6e32952bea953</originalsourceid><addsrcrecordid>eNpdUE1rFTEUDaJgfbp2JQQE0cW0-Zhkknb1KPVZeLQLdT3cZJJ5KdNknGRa_U3-SfOsK1f3wD1fHITeUnJKCeNn23NKiJZUtpQrJZ-hEyo61uhOqecVc0Ua3rX8JXqV8x0hpNNSnqDfVz_nxeUcUsTJ43JwOMTsbAk2DDDh6Qgj9ku6xzmmx2FJM_64gwliOawZx_AAU8gYxnFaKzPEC7y72X6qLrgsEPPoYrD48eCg4PmoyufYeV9tM66ZNXyAckw3v_6mjwtUKcyHMOCvoSRz_MGDi-Beoxcepuze_Lsb9P3z1bfLL83-dnd9ud03lglVGqE7A63zTBljgVg3tKA7yoWAgbOBSW-cFkYPbevrCsZbY6XjTAtmHGjBN-jDk--8pB-ry6W_D9m6qbZ3ac097RiRqm6-Qe__I96ldYm1W8-YqH5cKlpZ755YHlIP4xJyf7OnWmtCGK29_gABHom4</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2259523681</pqid></control><display><type>article</type><title>Expression of the insecticidal lectin from snowdrop (Galanthus nivalis agglutinin; GNA) in transgenic wheat plants: effects on predation by the grain aphid Sitobion avenae</title><source>SpringerNature Journals</source><creator>Stoger, E. (John Innes Centre, Norwich (United Kingdom).) ; Williams, S ; Christou, P ; Down, R.E ; Gatehouse, J.A</creator><creatorcontrib>Stoger, E. (John Innes Centre, Norwich (United Kingdom).) ; Williams, S ; Christou, P ; Down, R.E ; Gatehouse, J.A</creatorcontrib><description>Transgenic wheat plants containing the gene encoding snowdrop lectin (Galanthus nivalis agglutinin; GNA) under the control of constitutive and phloem-specific promoters were generated through the particle bombardment method. Thirty-two independently derived plants were subjected to molecular and biochemical analyses. Transgene integration varied from one to twelve estimated copies per haploid genome, and levels of GNA expression from 0 to ca. 0.2% of total soluble protein were observed in different transgenic plants. Seven transgenic plants were selected for further study. Progeny plants from these parental transformants were selected for transgene expression, and tested for enhanced resistance to the grain aphid (Sitobion avenae) by exposing the plants to nymphal insects under glasshouse conditions. Bioassay results show that transgenic wheat plants from lines expressing GNA at levels greater than ca. 0.04% of total soluble protein decrease the fecundity, but not the survival, of grain aphids. We propose that transgenic approaches using insecticidal genes such as gna in combination with integrated pest management present promising opportunities for the control of damaging wheat pests.</description><identifier>ISSN: 1380-3743</identifier><identifier>EISSN: 1572-9788</identifier><identifier>DOI: 10.1023/A:1009616413886</identifier><language>eng</language><publisher>Dordrecht: Springer Nature B.V</publisher><subject>AGGLUTININE ; AGGLUTININS ; AGLUTININAS ; Agricultural practices ; Aphidoidea ; Bioassays ; Bombardment ; EXPRESION GENICA ; EXPRESSION DES GENES ; Fecundity ; GALANTHUS ; Galanthus nivalis ; GENE EXPRESSION ; Genomes ; Greenhouses ; Insecticide resistance ; Insects ; Integrated pest management ; LECTINAS ; LECTINE ; LECTINS ; Molecular biology ; Particle bombardment ; Pest control ; PEST RESISTANCE ; PESTICIDAL PROPERTIES ; Pests ; Plant biology ; PLANTAS TRANSGENICAS ; PLANTE TRANSGENIQUE ; Plants (botany) ; Predation ; Progeny ; PROPIEDADES PLAGUICIDAS ; PROPRIETE DES PESTICIDES ; Proteins ; RESISTANCE AUX ORGANISMES NUISIBLES ; RESISTENCIA A LAS PLAGAS ; SITOBION AVENAE ; TRANSGENIC PLANTS ; TRITICUM AESTIVUM ; Wheat</subject><ispartof>Molecular breeding, 1999, Vol.5 (1), p.65-73</ispartof><rights>Molecular Breeding is a copyright of Springer, (1999). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c258t-597ba4ef28bbca0ced4a971355ad32d26fbe95b9d44f079bfcbc6e32952bea953</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,782,786,27933,27934</link.rule.ids></links><search><creatorcontrib>Stoger, E. (John Innes Centre, Norwich (United Kingdom).)</creatorcontrib><creatorcontrib>Williams, S</creatorcontrib><creatorcontrib>Christou, P</creatorcontrib><creatorcontrib>Down, R.E</creatorcontrib><creatorcontrib>Gatehouse, J.A</creatorcontrib><title>Expression of the insecticidal lectin from snowdrop (Galanthus nivalis agglutinin; GNA) in transgenic wheat plants: effects on predation by the grain aphid Sitobion avenae</title><title>Molecular breeding</title><description>Transgenic wheat plants containing the gene encoding snowdrop lectin (Galanthus nivalis agglutinin; GNA) under the control of constitutive and phloem-specific promoters were generated through the particle bombardment method. Thirty-two independently derived plants were subjected to molecular and biochemical analyses. Transgene integration varied from one to twelve estimated copies per haploid genome, and levels of GNA expression from 0 to ca. 0.2% of total soluble protein were observed in different transgenic plants. Seven transgenic plants were selected for further study. Progeny plants from these parental transformants were selected for transgene expression, and tested for enhanced resistance to the grain aphid (Sitobion avenae) by exposing the plants to nymphal insects under glasshouse conditions. Bioassay results show that transgenic wheat plants from lines expressing GNA at levels greater than ca. 0.04% of total soluble protein decrease the fecundity, but not the survival, of grain aphids. We propose that transgenic approaches using insecticidal genes such as gna in combination with integrated pest management present promising opportunities for the control of damaging wheat pests.</description><subject>AGGLUTININE</subject><subject>AGGLUTININS</subject><subject>AGLUTININAS</subject><subject>Agricultural practices</subject><subject>Aphidoidea</subject><subject>Bioassays</subject><subject>Bombardment</subject><subject>EXPRESION GENICA</subject><subject>EXPRESSION DES GENES</subject><subject>Fecundity</subject><subject>GALANTHUS</subject><subject>Galanthus nivalis</subject><subject>GENE EXPRESSION</subject><subject>Genomes</subject><subject>Greenhouses</subject><subject>Insecticide resistance</subject><subject>Insects</subject><subject>Integrated pest management</subject><subject>LECTINAS</subject><subject>LECTINE</subject><subject>LECTINS</subject><subject>Molecular biology</subject><subject>Particle bombardment</subject><subject>Pest control</subject><subject>PEST RESISTANCE</subject><subject>PESTICIDAL PROPERTIES</subject><subject>Pests</subject><subject>Plant biology</subject><subject>PLANTAS TRANSGENICAS</subject><subject>PLANTE TRANSGENIQUE</subject><subject>Plants (botany)</subject><subject>Predation</subject><subject>Progeny</subject><subject>PROPIEDADES PLAGUICIDAS</subject><subject>PROPRIETE DES PESTICIDES</subject><subject>Proteins</subject><subject>RESISTANCE AUX ORGANISMES NUISIBLES</subject><subject>RESISTENCIA A LAS PLAGAS</subject><subject>SITOBION AVENAE</subject><subject>TRANSGENIC PLANTS</subject><subject>TRITICUM AESTIVUM</subject><subject>Wheat</subject><issn>1380-3743</issn><issn>1572-9788</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpdUE1rFTEUDaJgfbp2JQQE0cW0-Zhkknb1KPVZeLQLdT3cZJJ5KdNknGRa_U3-SfOsK1f3wD1fHITeUnJKCeNn23NKiJZUtpQrJZ-hEyo61uhOqecVc0Ua3rX8JXqV8x0hpNNSnqDfVz_nxeUcUsTJ43JwOMTsbAk2DDDh6Qgj9ku6xzmmx2FJM_64gwliOawZx_AAU8gYxnFaKzPEC7y72X6qLrgsEPPoYrD48eCg4PmoyufYeV9tM66ZNXyAckw3v_6mjwtUKcyHMOCvoSRz_MGDi-Beoxcepuze_Lsb9P3z1bfLL83-dnd9ud03lglVGqE7A63zTBljgVg3tKA7yoWAgbOBSW-cFkYPbevrCsZbY6XjTAtmHGjBN-jDk--8pB-ry6W_D9m6qbZ3ac097RiRqm6-Qe__I96ldYm1W8-YqH5cKlpZ755YHlIP4xJyf7OnWmtCGK29_gABHom4</recordid><startdate>1999</startdate><enddate>1999</enddate><creator>Stoger, E. (John Innes Centre, Norwich (United Kingdom).)</creator><creator>Williams, S</creator><creator>Christou, P</creator><creator>Down, R.E</creator><creator>Gatehouse, J.A</creator><general>Springer Nature B.V</general><scope>FBQ</scope><scope>3V.</scope><scope>7X2</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>1999</creationdate><title>Expression of the insecticidal lectin from snowdrop (Galanthus nivalis agglutinin; GNA) in transgenic wheat plants: effects on predation by the grain aphid Sitobion avenae</title><author>Stoger, E. (John Innes Centre, Norwich (United Kingdom).) ; Williams, S ; Christou, P ; Down, R.E ; Gatehouse, J.A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c258t-597ba4ef28bbca0ced4a971355ad32d26fbe95b9d44f079bfcbc6e32952bea953</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>AGGLUTININE</topic><topic>AGGLUTININS</topic><topic>AGLUTININAS</topic><topic>Agricultural practices</topic><topic>Aphidoidea</topic><topic>Bioassays</topic><topic>Bombardment</topic><topic>EXPRESION GENICA</topic><topic>EXPRESSION DES GENES</topic><topic>Fecundity</topic><topic>GALANTHUS</topic><topic>Galanthus nivalis</topic><topic>GENE EXPRESSION</topic><topic>Genomes</topic><topic>Greenhouses</topic><topic>Insecticide resistance</topic><topic>Insects</topic><topic>Integrated pest management</topic><topic>LECTINAS</topic><topic>LECTINE</topic><topic>LECTINS</topic><topic>Molecular biology</topic><topic>Particle bombardment</topic><topic>Pest control</topic><topic>PEST RESISTANCE</topic><topic>PESTICIDAL PROPERTIES</topic><topic>Pests</topic><topic>Plant biology</topic><topic>PLANTAS TRANSGENICAS</topic><topic>PLANTE TRANSGENIQUE</topic><topic>Plants (botany)</topic><topic>Predation</topic><topic>Progeny</topic><topic>PROPIEDADES PLAGUICIDAS</topic><topic>PROPRIETE DES PESTICIDES</topic><topic>Proteins</topic><topic>RESISTANCE AUX ORGANISMES NUISIBLES</topic><topic>RESISTENCIA A LAS PLAGAS</topic><topic>SITOBION AVENAE</topic><topic>TRANSGENIC PLANTS</topic><topic>TRITICUM AESTIVUM</topic><topic>Wheat</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Stoger, E. (John Innes Centre, Norwich (United Kingdom).)</creatorcontrib><creatorcontrib>Williams, S</creatorcontrib><creatorcontrib>Christou, P</creatorcontrib><creatorcontrib>Down, R.E</creatorcontrib><creatorcontrib>Gatehouse, J.A</creatorcontrib><collection>AGRIS</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Molecular breeding</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Stoger, E. (John Innes Centre, Norwich (United Kingdom).)</au><au>Williams, S</au><au>Christou, P</au><au>Down, R.E</au><au>Gatehouse, J.A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of the insecticidal lectin from snowdrop (Galanthus nivalis agglutinin; GNA) in transgenic wheat plants: effects on predation by the grain aphid Sitobion avenae</atitle><jtitle>Molecular breeding</jtitle><date>1999</date><risdate>1999</risdate><volume>5</volume><issue>1</issue><spage>65</spage><epage>73</epage><pages>65-73</pages><issn>1380-3743</issn><eissn>1572-9788</eissn><abstract>Transgenic wheat plants containing the gene encoding snowdrop lectin (Galanthus nivalis agglutinin; GNA) under the control of constitutive and phloem-specific promoters were generated through the particle bombardment method. Thirty-two independently derived plants were subjected to molecular and biochemical analyses. Transgene integration varied from one to twelve estimated copies per haploid genome, and levels of GNA expression from 0 to ca. 0.2% of total soluble protein were observed in different transgenic plants. Seven transgenic plants were selected for further study. Progeny plants from these parental transformants were selected for transgene expression, and tested for enhanced resistance to the grain aphid (Sitobion avenae) by exposing the plants to nymphal insects under glasshouse conditions. Bioassay results show that transgenic wheat plants from lines expressing GNA at levels greater than ca. 0.04% of total soluble protein decrease the fecundity, but not the survival, of grain aphids. We propose that transgenic approaches using insecticidal genes such as gna in combination with integrated pest management present promising opportunities for the control of damaging wheat pests.</abstract><cop>Dordrecht</cop><pub>Springer Nature B.V</pub><doi>10.1023/A:1009616413886</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1380-3743 |
| ispartof | Molecular breeding, 1999, Vol.5 (1), p.65-73 |
| issn | 1380-3743 1572-9788 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_17206802 |
| source | SpringerNature Journals |
| subjects | AGGLUTININE AGGLUTININS AGLUTININAS Agricultural practices Aphidoidea Bioassays Bombardment EXPRESION GENICA EXPRESSION DES GENES Fecundity GALANTHUS Galanthus nivalis GENE EXPRESSION Genomes Greenhouses Insecticide resistance Insects Integrated pest management LECTINAS LECTINE LECTINS Molecular biology Particle bombardment Pest control PEST RESISTANCE PESTICIDAL PROPERTIES Pests Plant biology PLANTAS TRANSGENICAS PLANTE TRANSGENIQUE Plants (botany) Predation Progeny PROPIEDADES PLAGUICIDAS PROPRIETE DES PESTICIDES Proteins RESISTANCE AUX ORGANISMES NUISIBLES RESISTENCIA A LAS PLAGAS SITOBION AVENAE TRANSGENIC PLANTS TRITICUM AESTIVUM Wheat |
| title | Expression of the insecticidal lectin from snowdrop (Galanthus nivalis agglutinin; GNA) in transgenic wheat plants: effects on predation by the grain aphid Sitobion avenae |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-02T00%3A58%3A09IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_fao_a&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Expression%20of%20the%20insecticidal%20lectin%20from%20snowdrop%20(Galanthus%20nivalis%20agglutinin;%20GNA)%20in%20transgenic%20wheat%20plants:%20effects%20on%20predation%20by%20the%20grain%20aphid%20Sitobion%20avenae&rft.jtitle=Molecular%20breeding&rft.au=Stoger,%20E.%20(John%20Innes%20Centre,%20Norwich%20(United%20Kingdom).)&rft.date=1999&rft.volume=5&rft.issue=1&rft.spage=65&rft.epage=73&rft.pages=65-73&rft.issn=1380-3743&rft.eissn=1572-9788&rft_id=info:doi/10.1023/A:1009616413886&rft_dat=%3Cproquest_fao_a%3E17206802%3C/proquest_fao_a%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2259523681&rft_id=info:pmid/&rfr_iscdi=true |