Anti-tumor and immunomodulating effects of Pleurotus ostreatus mycelia-derived proteoglycans
Pleurotus ostreatus is one of the widely cultivated edible mushrooms. Water-soluble proteoglycan fractions from P. ostreatus mycelia were purified by alcohol-precipitation, ion exchange and followed by gel permeation (Sephadex G-100) chromatography. Three neutral fractions were found, which had poly...
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| Veröffentlicht in: | International immunopharmacology 2006-08, Vol.6 (8), p.1287-1297 |
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| creator | Sarangi, Itisam Ghosh, Dipanjan Bhutia, Sujit Kumar Mallick, Sanjaya Kumar Maiti, Tapas K. |
| description | Pleurotus ostreatus is one of the widely cultivated edible mushrooms. Water-soluble proteoglycan fractions from
P. ostreatus mycelia were purified by alcohol-precipitation, ion exchange and followed by gel permeation (Sephadex G-100) chromatography. Three neutral fractions were found, which had polysaccharide to protein ratios 14.2, 26.4 and 18.3, respectively. These fractions were tested for in vitro and in vivo immunomodulatory and anticancer effects on Sarcoma-180-bearing mouse model. In vivo injection of proteoglycans to Sarcoma-180-bearing mice decreased the number of tumor cells and cell cycle analysis showed that most of the cells were found to be arrested in pre-G
0/G
1 phase of cell cycle. All of the three proteoglycans elevated mouse natural killer (NK) cell cytotoxicity and stimulated macrophages to produce nitric oxide. The Fourier transform infra red (FTIR) spectra suggested the presence of beta-glycosidic bond in all the fractions. Fraction I strongly interacted with glucose/mannose-specific lectin Concanavalin A (ConA), indicating the presence of large number of terminal sugar with glucose/mannose. Thus, the three neutral proteoglycans derived from the mushroom (
P. ostreatus) mycelia could be used as immunomodulators and anti cancer agents. |
| doi_str_mv | 10.1016/j.intimp.2006.04.002 |
| format | Article |
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P. ostreatus mycelia were purified by alcohol-precipitation, ion exchange and followed by gel permeation (Sephadex G-100) chromatography. Three neutral fractions were found, which had polysaccharide to protein ratios 14.2, 26.4 and 18.3, respectively. These fractions were tested for in vitro and in vivo immunomodulatory and anticancer effects on Sarcoma-180-bearing mouse model. In vivo injection of proteoglycans to Sarcoma-180-bearing mice decreased the number of tumor cells and cell cycle analysis showed that most of the cells were found to be arrested in pre-G
0/G
1 phase of cell cycle. All of the three proteoglycans elevated mouse natural killer (NK) cell cytotoxicity and stimulated macrophages to produce nitric oxide. The Fourier transform infra red (FTIR) spectra suggested the presence of beta-glycosidic bond in all the fractions. Fraction I strongly interacted with glucose/mannose-specific lectin Concanavalin A (ConA), indicating the presence of large number of terminal sugar with glucose/mannose. Thus, the three neutral proteoglycans derived from the mushroom (
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P. ostreatus mycelia were purified by alcohol-precipitation, ion exchange and followed by gel permeation (Sephadex G-100) chromatography. Three neutral fractions were found, which had polysaccharide to protein ratios 14.2, 26.4 and 18.3, respectively. These fractions were tested for in vitro and in vivo immunomodulatory and anticancer effects on Sarcoma-180-bearing mouse model. In vivo injection of proteoglycans to Sarcoma-180-bearing mice decreased the number of tumor cells and cell cycle analysis showed that most of the cells were found to be arrested in pre-G
0/G
1 phase of cell cycle. All of the three proteoglycans elevated mouse natural killer (NK) cell cytotoxicity and stimulated macrophages to produce nitric oxide. The Fourier transform infra red (FTIR) spectra suggested the presence of beta-glycosidic bond in all the fractions. Fraction I strongly interacted with glucose/mannose-specific lectin Concanavalin A (ConA), indicating the presence of large number of terminal sugar with glucose/mannose. Thus, the three neutral proteoglycans derived from the mushroom (
P. ostreatus) mycelia could be used as immunomodulators and anti cancer agents.</description><subject>Animals</subject><subject>Anti-cancer</subject><subject>Antineoplastic Agents - chemistry</subject><subject>Antineoplastic Agents - immunology</subject><subject>Antineoplastic Agents - therapeutic use</subject><subject>Biological and medical sciences</subject><subject>Cell Cycle - drug effects</subject><subject>Cell Line, Tumor</subject><subject>Cell Proliferation - drug effects</subject><subject>Cell Survival - drug effects</subject><subject>Chemical Fractionation - methods</subject><subject>Cytotoxicity Tests, Immunologic</subject><subject>Dose-Response Relationship, Drug</subject><subject>Immunomodulatory</subject><subject>Killer Cells, Natural - cytology</subject><subject>Killer Cells, Natural - drug effects</subject><subject>Killer Cells, Natural - immunology</subject><subject>L-Lactate Dehydrogenase - analysis</subject><subject>Lectins - metabolism</subject><subject>Macrophages, Peritoneal - drug effects</subject><subject>Macrophages, Peritoneal - metabolism</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mushroom</subject><subject>Mycelia</subject><subject>Mycelium - chemistry</subject><subject>Nitric Oxide - metabolism</subject><subject>Pharmacology. Drug treatments</subject><subject>Pleurotus - chemistry</subject><subject>Pleurotus ostreatus</subject><subject>Protein Binding</subject><subject>Proteoglycan</subject><subject>Proteoglycans - isolation & purification</subject><subject>Proteoglycans - metabolism</subject><subject>Proteoglycans - therapeutic use</subject><subject>Sarcoma 180 - drug therapy</subject><subject>Sarcoma 180 - immunology</subject><subject>Sarcoma 180 - pathology</subject><subject>Solubility</subject><subject>Spleen - cytology</subject><subject>Spleen - drug effects</subject><issn>1567-5769</issn><issn>1878-1705</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMFqGzEQhkVJSdw0b1DCXprbbqW1VtJeCsYkaSHQHtpbQYylUZBZrRxp1-C3r4wNvuU0M-KbX8NHyBdGG0aZ-LZt_Dj5sGtaSkVDeUNp-4EsmJKqZpJ2V6XvhKw7Kfob8innLaXlnbNrcsOEVG3H2YL8W5WQeppDTBWMtvIhzGMM0c4DTH58rdA5NFOuoqt-DzinOM1lyFNCOHbhYHDwUFtMfo-22hUA4-twMDDmz-SjgyHj3bnekr9Pj3_WP-qXX88_16uX2nAlpxplt7TOwYY7Z40CYcG4tu0sX6JTykqKyJXpe95Bq9qNYGDRwIaCAs6ZW96Sh1Nu-f1txjzp4HO5a4AR45w1k8XRktEC8hNoUsw5odO75AOkg2ZUH63qrT5Z1UermnJdrJa1-3P-vAloL0tnjQX4egYgGxhcgtH4fOGkUqIXfeG-nzgsNvYek87G42jQ-lQsaxv9-5f8B6BYm1w</recordid><startdate>20060801</startdate><enddate>20060801</enddate><creator>Sarangi, Itisam</creator><creator>Ghosh, Dipanjan</creator><creator>Bhutia, Sujit Kumar</creator><creator>Mallick, Sanjaya Kumar</creator><creator>Maiti, Tapas K.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T5</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope></search><sort><creationdate>20060801</creationdate><title>Anti-tumor and immunomodulating effects of Pleurotus ostreatus mycelia-derived proteoglycans</title><author>Sarangi, Itisam ; Ghosh, Dipanjan ; Bhutia, Sujit Kumar ; Mallick, Sanjaya Kumar ; Maiti, Tapas K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c487t-e753dffab4ffdc8a6dacf225d43ef88d70ee48c9945a282b61adecab0a8a441f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Anti-cancer</topic><topic>Antineoplastic Agents - chemistry</topic><topic>Antineoplastic Agents - immunology</topic><topic>Antineoplastic Agents - therapeutic use</topic><topic>Biological and medical sciences</topic><topic>Cell Cycle - drug effects</topic><topic>Cell Line, Tumor</topic><topic>Cell Proliferation - drug effects</topic><topic>Cell Survival - drug effects</topic><topic>Chemical Fractionation - methods</topic><topic>Cytotoxicity Tests, Immunologic</topic><topic>Dose-Response Relationship, Drug</topic><topic>Immunomodulatory</topic><topic>Killer Cells, Natural - cytology</topic><topic>Killer Cells, Natural - drug effects</topic><topic>Killer Cells, Natural - immunology</topic><topic>L-Lactate Dehydrogenase - analysis</topic><topic>Lectins - metabolism</topic><topic>Macrophages, Peritoneal - drug effects</topic><topic>Macrophages, Peritoneal - metabolism</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mushroom</topic><topic>Mycelia</topic><topic>Mycelium - chemistry</topic><topic>Nitric Oxide - metabolism</topic><topic>Pharmacology. Drug treatments</topic><topic>Pleurotus - chemistry</topic><topic>Pleurotus ostreatus</topic><topic>Protein Binding</topic><topic>Proteoglycan</topic><topic>Proteoglycans - isolation & purification</topic><topic>Proteoglycans - metabolism</topic><topic>Proteoglycans - therapeutic use</topic><topic>Sarcoma 180 - drug therapy</topic><topic>Sarcoma 180 - immunology</topic><topic>Sarcoma 180 - pathology</topic><topic>Solubility</topic><topic>Spleen - cytology</topic><topic>Spleen - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sarangi, Itisam</creatorcontrib><creatorcontrib>Ghosh, Dipanjan</creatorcontrib><creatorcontrib>Bhutia, Sujit Kumar</creatorcontrib><creatorcontrib>Mallick, Sanjaya Kumar</creatorcontrib><creatorcontrib>Maiti, Tapas K.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>International immunopharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sarangi, Itisam</au><au>Ghosh, Dipanjan</au><au>Bhutia, Sujit Kumar</au><au>Mallick, Sanjaya Kumar</au><au>Maiti, Tapas K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Anti-tumor and immunomodulating effects of Pleurotus ostreatus mycelia-derived proteoglycans</atitle><jtitle>International immunopharmacology</jtitle><addtitle>Int Immunopharmacol</addtitle><date>2006-08-01</date><risdate>2006</risdate><volume>6</volume><issue>8</issue><spage>1287</spage><epage>1297</epage><pages>1287-1297</pages><issn>1567-5769</issn><eissn>1878-1705</eissn><abstract>Pleurotus ostreatus is one of the widely cultivated edible mushrooms. Water-soluble proteoglycan fractions from
P. ostreatus mycelia were purified by alcohol-precipitation, ion exchange and followed by gel permeation (Sephadex G-100) chromatography. Three neutral fractions were found, which had polysaccharide to protein ratios 14.2, 26.4 and 18.3, respectively. These fractions were tested for in vitro and in vivo immunomodulatory and anticancer effects on Sarcoma-180-bearing mouse model. In vivo injection of proteoglycans to Sarcoma-180-bearing mice decreased the number of tumor cells and cell cycle analysis showed that most of the cells were found to be arrested in pre-G
0/G
1 phase of cell cycle. All of the three proteoglycans elevated mouse natural killer (NK) cell cytotoxicity and stimulated macrophages to produce nitric oxide. The Fourier transform infra red (FTIR) spectra suggested the presence of beta-glycosidic bond in all the fractions. Fraction I strongly interacted with glucose/mannose-specific lectin Concanavalin A (ConA), indicating the presence of large number of terminal sugar with glucose/mannose. Thus, the three neutral proteoglycans derived from the mushroom (
P. ostreatus) mycelia could be used as immunomodulators and anti cancer agents.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>16782541</pmid><doi>10.1016/j.intimp.2006.04.002</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | International immunopharmacology, 2006-08, Vol.6 (8), p.1287-1297 |
| issn | 1567-5769 1878-1705 |
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| subjects | Animals Anti-cancer Antineoplastic Agents - chemistry Antineoplastic Agents - immunology Antineoplastic Agents - therapeutic use Biological and medical sciences Cell Cycle - drug effects Cell Line, Tumor Cell Proliferation - drug effects Cell Survival - drug effects Chemical Fractionation - methods Cytotoxicity Tests, Immunologic Dose-Response Relationship, Drug Immunomodulatory Killer Cells, Natural - cytology Killer Cells, Natural - drug effects Killer Cells, Natural - immunology L-Lactate Dehydrogenase - analysis Lectins - metabolism Macrophages, Peritoneal - drug effects Macrophages, Peritoneal - metabolism Medical sciences Mice Mushroom Mycelia Mycelium - chemistry Nitric Oxide - metabolism Pharmacology. Drug treatments Pleurotus - chemistry Pleurotus ostreatus Protein Binding Proteoglycan Proteoglycans - isolation & purification Proteoglycans - metabolism Proteoglycans - therapeutic use Sarcoma 180 - drug therapy Sarcoma 180 - immunology Sarcoma 180 - pathology Solubility Spleen - cytology Spleen - drug effects |
| title | Anti-tumor and immunomodulating effects of Pleurotus ostreatus mycelia-derived proteoglycans |
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