Directed evolution of thymidine kinase for AZT phosphorylation using DNA family shuffling

The thymidine kinase (TK) genes from herpes simplex virus (HSV) types 1 and 2 were recombined in vitro with a technique called DNA family shuffling. A high-throughput robotic screen identified chimeras with an enhanced ability to phosphorylate zidovudine (AZT). Improved clones were combined, reshuff...

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Veröffentlicht in:	Nature biotechnology 1999-03, Vol.17 (3), p.259-264
Hauptverfasser:	Stemmer, Willem P.C, Christians, Fred C, Scapozza, Leonardo, Crameri, Andreas, Folkers, Gerd
Format:	Artikel
Sprache:	eng
Schlagworte:	Agriculture Amino Acid Sequence Bioinformatics Biological and medical sciences Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biomedicine Biotechnology Cell Division - drug effects Chimera Cloning, Molecular - methods Computer Simulation Dose-Response Relationship, Drug Escherichia coli - metabolism Fundamental and applied biological sciences. Psychology Gene therapy Health. Pharmaceutical industry Herpesvirus 1, Human - enzymology Herpesvirus 1, Human - genetics Herpesvirus 2, Human - enzymology Herpesvirus 2, Human - genetics Humans Hydrogen Bonding Industrial applications and implications. Economical aspects Kinetics Life Sciences Models, Molecular Molecular Sequence Data Mutagenesis research-article Sequence Homology, Amino Acid Thymidine Kinase - genetics Thymidine Kinase - metabolism Thymidine Kinase - therapeutic use Zidovudine - metabolism Zidovudine - pharmacology Zidovudine - therapeutic use
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