Proteomic analysis of Corylus heterophylla fisch in Changbai Mountain by shotgun approach
Total proteins of Changbai Mountain hazelnut were extracted and then enzymolysed in solution. The mixture of peptides was separated on chromatograph and identified by shotgun proteomics approach. The identified proteins were analyzed by bioinformatics, 303 proteins were identified, of which 237 prot...
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| Veröffentlicht in: | Pakistan journal of pharmaceutical sciences 2015-07, Vol.28 (4 Suppl), p.1449-1456 |
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| container_issue | 4 Suppl |
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| container_title | Pakistan journal of pharmaceutical sciences |
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| creator | Chunlei, Liu Weihong, Min Jingsheng, Liu Yan, Liu Jingxi, Ma Jingjing, Li |
| description | Total proteins of Changbai Mountain hazelnut were extracted and then enzymolysed in solution. The mixture of peptides was separated on chromatograph and identified by shotgun proteomics approach. The identified proteins were analyzed by bioinformatics, 303 proteins were identified, of which 237 proteins (78.2%) were mainly distributing in a range of 10-70 kDa and 85 proteins (28.1%) were around pI 5-6. Based on the biological process cellular component and molecular function, these proteins were classified by Gene Ontology, the results showed that 183 proteins (73.8%) had catalytic activity (e.g. oxidoreductase, kinase, peptidase, etc.), and 170 proteins (68.5%) had binding activity. The protein profile provided a valuable basis for further research of hazelnut proteins and opened up new research avenues related to the function of these proteins. |
| format | Article |
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The mixture of peptides was separated on chromatograph and identified by shotgun proteomics approach. The identified proteins were analyzed by bioinformatics, 303 proteins were identified, of which 237 proteins (78.2%) were mainly distributing in a range of 10-70 kDa and 85 proteins (28.1%) were around pI 5-6. Based on the biological process cellular component and molecular function, these proteins were classified by Gene Ontology, the results showed that 183 proteins (73.8%) had catalytic activity (e.g. oxidoreductase, kinase, peptidase, etc.), and 170 proteins (68.5%) had binding activity. The protein profile provided a valuable basis for further research of hazelnut proteins and opened up new research avenues related to the function of these proteins.</description><identifier>ISSN: 1011-601X</identifier><identifier>PMID: 26431657</identifier><language>eng</language><publisher>Pakistan: Pakistan Journal of Pharmaceutical Sciences</publisher><subject>Botanical research ; Corylus - chemistry ; Hazelnuts ; Methods ; Plant biochemistry ; Plant proteins ; Properties ; Proteomics ; Proteomics - methods</subject><ispartof>Pakistan journal of pharmaceutical sciences, 2015-07, Vol.28 (4 Suppl), p.1449-1456</ispartof><rights>COPYRIGHT 2015 Pakistan Journal of Pharmaceutical Sciences</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26431657$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chunlei, Liu</creatorcontrib><creatorcontrib>Weihong, Min</creatorcontrib><creatorcontrib>Jingsheng, Liu</creatorcontrib><creatorcontrib>Yan, Liu</creatorcontrib><creatorcontrib>Jingxi, Ma</creatorcontrib><creatorcontrib>Jingjing, Li</creatorcontrib><title>Proteomic analysis of Corylus heterophylla fisch in Changbai Mountain by shotgun approach</title><title>Pakistan journal of pharmaceutical sciences</title><addtitle>Pak J Pharm Sci</addtitle><description>Total proteins of Changbai Mountain hazelnut were extracted and then enzymolysed in solution. The mixture of peptides was separated on chromatograph and identified by shotgun proteomics approach. The identified proteins were analyzed by bioinformatics, 303 proteins were identified, of which 237 proteins (78.2%) were mainly distributing in a range of 10-70 kDa and 85 proteins (28.1%) were around pI 5-6. Based on the biological process cellular component and molecular function, these proteins were classified by Gene Ontology, the results showed that 183 proteins (73.8%) had catalytic activity (e.g. oxidoreductase, kinase, peptidase, etc.), and 170 proteins (68.5%) had binding activity. The protein profile provided a valuable basis for further research of hazelnut proteins and opened up new research avenues related to the function of these proteins.</description><subject>Botanical research</subject><subject>Corylus - chemistry</subject><subject>Hazelnuts</subject><subject>Methods</subject><subject>Plant biochemistry</subject><subject>Plant proteins</subject><subject>Properties</subject><subject>Proteomics</subject><subject>Proteomics - methods</subject><issn>1011-601X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1UE1PhDAQ5aBx19W_YHr0gmHa0tLjhviVrNHDHvRESilQAy22cODfW7Nr5jDJmzfvvZmLZAsZQMoy-Nwk1yF8ZxmjQoirZIMZJcByvk2-PrybtRuNQtLKYQ0mINei0vl1WALq9ay9m_p1GCRqTVA9MhaVvbRdLQ16c4udZUTqFYXezd1ikZwm76Tqb5LLVg5B3577Ljk-PR7Ll_Tw_vxa7g9ph_NsTqkShGGi6oLzpuAMQDUKmNaK5BSgkLUQHEsMDASTBc1FQyBOc5oXPGNkl9yfZKPrz6LDXI0xpo55rXZLqICDoBgDoZH6cKJ2ctCVsa2bvVSxGh3vd1a3JuJ7igueY1L8LdydtZd61E01eTNKv1b_7yO_FJdqWg</recordid><startdate>201507</startdate><enddate>201507</enddate><creator>Chunlei, Liu</creator><creator>Weihong, Min</creator><creator>Jingsheng, Liu</creator><creator>Yan, Liu</creator><creator>Jingxi, Ma</creator><creator>Jingjing, Li</creator><general>Pakistan Journal of Pharmaceutical Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>201507</creationdate><title>Proteomic analysis of Corylus heterophylla fisch in Changbai Mountain by shotgun approach</title><author>Chunlei, Liu ; Weihong, Min ; Jingsheng, Liu ; Yan, Liu ; Jingxi, Ma ; Jingjing, Li</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-g250t-4c93623cb877d87611cdc16eec354118ab9972a216196a8459d31eec54587063</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Botanical research</topic><topic>Corylus - chemistry</topic><topic>Hazelnuts</topic><topic>Methods</topic><topic>Plant biochemistry</topic><topic>Plant proteins</topic><topic>Properties</topic><topic>Proteomics</topic><topic>Proteomics - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chunlei, Liu</creatorcontrib><creatorcontrib>Weihong, Min</creatorcontrib><creatorcontrib>Jingsheng, Liu</creatorcontrib><creatorcontrib>Yan, Liu</creatorcontrib><creatorcontrib>Jingxi, Ma</creatorcontrib><creatorcontrib>Jingjing, Li</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Pakistan journal of pharmaceutical sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chunlei, Liu</au><au>Weihong, Min</au><au>Jingsheng, Liu</au><au>Yan, Liu</au><au>Jingxi, Ma</au><au>Jingjing, Li</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Proteomic analysis of Corylus heterophylla fisch in Changbai Mountain by shotgun approach</atitle><jtitle>Pakistan journal of pharmaceutical sciences</jtitle><addtitle>Pak J Pharm Sci</addtitle><date>2015-07</date><risdate>2015</risdate><volume>28</volume><issue>4 Suppl</issue><spage>1449</spage><epage>1456</epage><pages>1449-1456</pages><issn>1011-601X</issn><abstract>Total proteins of Changbai Mountain hazelnut were extracted and then enzymolysed in solution. The mixture of peptides was separated on chromatograph and identified by shotgun proteomics approach. The identified proteins were analyzed by bioinformatics, 303 proteins were identified, of which 237 proteins (78.2%) were mainly distributing in a range of 10-70 kDa and 85 proteins (28.1%) were around pI 5-6. Based on the biological process cellular component and molecular function, these proteins were classified by Gene Ontology, the results showed that 183 proteins (73.8%) had catalytic activity (e.g. oxidoreductase, kinase, peptidase, etc.), and 170 proteins (68.5%) had binding activity. The protein profile provided a valuable basis for further research of hazelnut proteins and opened up new research avenues related to the function of these proteins.</abstract><cop>Pakistan</cop><pub>Pakistan Journal of Pharmaceutical Sciences</pub><pmid>26431657</pmid><tpages>8</tpages></addata></record> |
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| subjects | Botanical research Corylus - chemistry Hazelnuts Methods Plant biochemistry Plant proteins Properties Proteomics Proteomics - methods |
| title | Proteomic analysis of Corylus heterophylla fisch in Changbai Mountain by shotgun approach |
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