Preparative isolation of polymerase chain reaction products using mixed-mode chromatography

The polymerase chain reaction (PCR) has become one of the most useful techniques in molecular biology laboratories around the world. The purification of the target DNA product is often challenging, however, and most users are restricted to employing available commercial kits. The recent developments...

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Veröffentlicht in:	Analytical biochemistry 2015-11, Vol.489, p.73-75
Hauptverfasser:	Matos, T., Silva, G., Queiroz, J.A., Bülow, L.
Format:	Artikel
Sprache:	eng
Schlagworte:	Chromatography, High Pressure Liquid Chromatography, Ion Exchange DNA - chemistry DNA - isolation & purification DNA - metabolism DNA, Plant - chemistry DNA, Plant - isolation & purification DNA, Plant - metabolism DNA, Recombinant - chemistry DNA, Recombinant - isolation & purification DNA, Recombinant - metabolism DNA, Single-Stranded - chemistry DNA, Single-Stranded - isolation & purification DNA, Single-Stranded - metabolism DNA, Viral - chemistry DNA, Viral - isolation & purification DNA, Viral - metabolism Double-stranded DNA Hydrophobic and Hydrophilic Interactions Hydrophobic interaction chromatography Ion-exchange chromatography Molecular Weight Plasmid DNA Polymerase Chain Reaction Reproducibility of Results Single-stranded DNA Spectrophotometry, Ultraviolet
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