One-step separation of β-galactosidase from β-lactoglobulin using water-in-oil microemulsions

► W/o microemulsions extract impurity proteins from β-galactosidase (β-gal) in one-step. ► Solubilisation studies determine microemulsion conditions for excluding β-gal. ► Mixtures contained β-gal and impurity protein (β-Lg) with similar pI but lower MW. ► Microemulsions with [buffer] >40mM and a...

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Veröffentlicht in:	Food chemistry 2012-05, Vol.132 (1), p.326-332
Hauptverfasser:	Mazı, Bekir G., Hamamcı, Haluk, Dungan, Stephanie R.
Format:	Artikel
Sprache:	eng
Schlagworte:	beta-Galactosidase - chemistry Biological and medical sciences Bioseparations Enzymes Extraction Food engineering Food industries Fundamental and applied biological sciences. Psychology General aspects Lactase Lactoglobulins - chemistry Micelles Reversed micelles Water
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