Optimized in vitro procedure for assessing the cytocompatibility of magnesium-based biomaterials
[Display omitted] Magnesium (Mg) is a promising biomaterial for degradable implant applications that has been extensively studied in vitro and in vivo in recent years. In this study, we developed a procedure that allows an optimized and uniform in vitro assessment of the cytocompatibility of Mg-base...
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| Veröffentlicht in: | Acta biomaterialia 2015-09, Vol.23, p.354-363 |
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| creator | Jung, Ole Smeets, Ralf Porchetta, Dario Kopp, Alexander Ptock, Christoph Müller, Ute Heiland, Max Schwade, Max Behr, Björn Kröger, Nadja Kluwe, Lan Hanken, Henning Hartjen, Philip |
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Magnesium (Mg) is a promising biomaterial for degradable implant applications that has been extensively studied in vitro and in vivo in recent years. In this study, we developed a procedure that allows an optimized and uniform in vitro assessment of the cytocompatibility of Mg-based materials while respecting the standard protocol DIN EN ISO 10993-5:2009. The mouse fibroblast line L-929 was chosen as the preferred assay cell line and MEM supplemented with 10% FCS, penicillin/streptomycin and 4mM l-glutamine as the favored assay medium. The procedure consists of (1) an indirect assessment of effects of soluble Mg corrosion products in material extracts and (2) a direct assessment of the surface compatibility in terms of cell attachment and cytotoxicity originating from active corrosion processes. The indirect assessment allows the quantification of cell-proliferation (BrdU-assay), viability (XTT-assay) as well as cytotoxicity (LDH-assay) of the mouse fibroblasts incubated with material extracts. Direct assessment visualizes cells attached to the test materials by means of live-dead staining. The colorimetric assays and the visual evaluation complement each other and the combination of both provides an optimized and simple procedure for assessing the cytocompatibility of Mg-based biomaterials in vitro. |
| doi_str_mv | 10.1016/j.actbio.2015.06.005 |
| format | Article |
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Magnesium (Mg) is a promising biomaterial for degradable implant applications that has been extensively studied in vitro and in vivo in recent years. In this study, we developed a procedure that allows an optimized and uniform in vitro assessment of the cytocompatibility of Mg-based materials while respecting the standard protocol DIN EN ISO 10993-5:2009. The mouse fibroblast line L-929 was chosen as the preferred assay cell line and MEM supplemented with 10% FCS, penicillin/streptomycin and 4mM l-glutamine as the favored assay medium. The procedure consists of (1) an indirect assessment of effects of soluble Mg corrosion products in material extracts and (2) a direct assessment of the surface compatibility in terms of cell attachment and cytotoxicity originating from active corrosion processes. The indirect assessment allows the quantification of cell-proliferation (BrdU-assay), viability (XTT-assay) as well as cytotoxicity (LDH-assay) of the mouse fibroblasts incubated with material extracts. Direct assessment visualizes cells attached to the test materials by means of live-dead staining. The colorimetric assays and the visual evaluation complement each other and the combination of both provides an optimized and simple procedure for assessing the cytocompatibility of Mg-based biomaterials in vitro.</description><identifier>ISSN: 1742-7061</identifier><identifier>EISSN: 1878-7568</identifier><identifier>DOI: 10.1016/j.actbio.2015.06.005</identifier><identifier>PMID: 26073090</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Algorithms ; Animals ; Assaying ; Assessments ; Biocompatible Materials - toxicity ; Biological Assay - methods ; Biological Assay - standards ; Biomaterials ; Biomedical materials ; Cell Line ; Cell Survival - drug effects ; Cytocompatibility ; Fibroblasts ; Fibroblasts - drug effects ; Fibroblasts - pathology ; Guidelines as Topic ; In vitro biocompatibility ; In vitro cytotoxicity ; In Vitro Techniques ; In vitro testing ; Internationality ; Magnesium ; Magnesium - toxicity ; Materials Testing - methods ; Materials Testing - standards ; Mice ; Plasma electrolytic oxidation (PEO) ; Surgical implants ; Toxicity Tests - methods ; Toxicity Tests - standards</subject><ispartof>Acta biomaterialia, 2015-09, Vol.23, p.354-363</ispartof><rights>2015 Acta Materialia Inc.</rights><rights>Copyright © 2015 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c535t-ea1ef549e8fb4bc567d6e56639a1589aee1886d4193b77511626f952b1d1f7cc3</citedby><cites>FETCH-LOGICAL-c535t-ea1ef549e8fb4bc567d6e56639a1589aee1886d4193b77511626f952b1d1f7cc3</cites><orcidid>0000-0001-7600-9181 ; 0000-0002-4987-2913</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1742706115002706$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26073090$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jung, Ole</creatorcontrib><creatorcontrib>Smeets, Ralf</creatorcontrib><creatorcontrib>Porchetta, Dario</creatorcontrib><creatorcontrib>Kopp, Alexander</creatorcontrib><creatorcontrib>Ptock, Christoph</creatorcontrib><creatorcontrib>Müller, Ute</creatorcontrib><creatorcontrib>Heiland, Max</creatorcontrib><creatorcontrib>Schwade, Max</creatorcontrib><creatorcontrib>Behr, Björn</creatorcontrib><creatorcontrib>Kröger, Nadja</creatorcontrib><creatorcontrib>Kluwe, Lan</creatorcontrib><creatorcontrib>Hanken, Henning</creatorcontrib><creatorcontrib>Hartjen, Philip</creatorcontrib><title>Optimized in vitro procedure for assessing the cytocompatibility of magnesium-based biomaterials</title><title>Acta biomaterialia</title><addtitle>Acta Biomater</addtitle><description>[Display omitted]
Magnesium (Mg) is a promising biomaterial for degradable implant applications that has been extensively studied in vitro and in vivo in recent years. In this study, we developed a procedure that allows an optimized and uniform in vitro assessment of the cytocompatibility of Mg-based materials while respecting the standard protocol DIN EN ISO 10993-5:2009. The mouse fibroblast line L-929 was chosen as the preferred assay cell line and MEM supplemented with 10% FCS, penicillin/streptomycin and 4mM l-glutamine as the favored assay medium. The procedure consists of (1) an indirect assessment of effects of soluble Mg corrosion products in material extracts and (2) a direct assessment of the surface compatibility in terms of cell attachment and cytotoxicity originating from active corrosion processes. The indirect assessment allows the quantification of cell-proliferation (BrdU-assay), viability (XTT-assay) as well as cytotoxicity (LDH-assay) of the mouse fibroblasts incubated with material extracts. Direct assessment visualizes cells attached to the test materials by means of live-dead staining. 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Magnesium (Mg) is a promising biomaterial for degradable implant applications that has been extensively studied in vitro and in vivo in recent years. In this study, we developed a procedure that allows an optimized and uniform in vitro assessment of the cytocompatibility of Mg-based materials while respecting the standard protocol DIN EN ISO 10993-5:2009. The mouse fibroblast line L-929 was chosen as the preferred assay cell line and MEM supplemented with 10% FCS, penicillin/streptomycin and 4mM l-glutamine as the favored assay medium. The procedure consists of (1) an indirect assessment of effects of soluble Mg corrosion products in material extracts and (2) a direct assessment of the surface compatibility in terms of cell attachment and cytotoxicity originating from active corrosion processes. The indirect assessment allows the quantification of cell-proliferation (BrdU-assay), viability (XTT-assay) as well as cytotoxicity (LDH-assay) of the mouse fibroblasts incubated with material extracts. Direct assessment visualizes cells attached to the test materials by means of live-dead staining. The colorimetric assays and the visual evaluation complement each other and the combination of both provides an optimized and simple procedure for assessing the cytocompatibility of Mg-based biomaterials in vitro.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>26073090</pmid><doi>10.1016/j.actbio.2015.06.005</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0001-7600-9181</orcidid><orcidid>https://orcid.org/0000-0002-4987-2913</orcidid></addata></record> |
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| ispartof | Acta biomaterialia, 2015-09, Vol.23, p.354-363 |
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| subjects | Algorithms Animals Assaying Assessments Biocompatible Materials - toxicity Biological Assay - methods Biological Assay - standards Biomaterials Biomedical materials Cell Line Cell Survival - drug effects Cytocompatibility Fibroblasts Fibroblasts - drug effects Fibroblasts - pathology Guidelines as Topic In vitro biocompatibility In vitro cytotoxicity In Vitro Techniques In vitro testing Internationality Magnesium Magnesium - toxicity Materials Testing - methods Materials Testing - standards Mice Plasma electrolytic oxidation (PEO) Surgical implants Toxicity Tests - methods Toxicity Tests - standards |
| title | Optimized in vitro procedure for assessing the cytocompatibility of magnesium-based biomaterials |
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