Temporal characterization of protein production levels from baculovirus vectors coding for GFP and RFP genes under non-conventional promoter control

ABSTRACT The ease of use and versatility of the Baculovirus Expression Vector System (BEVS) has made it one of the most widely used systems for recombinant protein production However, co‐expression systems currently in use mainly make use of the very strong very late p10 and polyhedron (polh) promot...

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Veröffentlicht in:	Biotechnology and bioengineering 2015-09, Vol.112 (9), p.1822-1831
Hauptverfasser:	George, Steve, Jauhar, Altamash M., Mackenzie, Jennifer, Kieβlich, Sascha, Aucoin, Marc G.
Format:	Artikel
Sprache:	eng
Schlagworte:	Autographa californica Baculoviridae - genetics Baculovirus baculovirus expression vector system BEVS Biotechnology Biotechnology - methods Cell Proliferation Cells co-expression Control systems Gene expression Genes Genes, Reporter - genetics Genetic Vectors - genetics Green Fluorescent Proteins - analysis Green Fluorescent Proteins - genetics Green Fluorescent Proteins - metabolism infection insect cell Insects Luminescent Proteins - analysis Luminescent Proteins - genetics Luminescent Proteins - metabolism Mathematical analysis Nuclear polyhedrosis virus promoter control Promoter Regions, Genetic - genetics Proteins Recombinant Proteins - analysis Recombinant Proteins - genetics Recombinant Proteins - metabolism Red Fluorescent Protein reporter protein Sf9 Cells Vectors (mathematics) Viruses
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