Enzyme switch by complementary polymer pair system (CPPS)
Manipulation of enzyme activity at will presents various research and industrial applications. Herein we describe development of a technology for inactivation and reactivation of enzyme activities using a polyanionic poly(acrylic acid) (PAAc) and a polycationic poly(allylamine) (PAA). Enzyme activit...
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| Veröffentlicht in: | Soft matter 2010-01, Vol.6 (21), p.5320-5326 |
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| container_title | Soft matter |
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| creator | Tomita, Shunsuke Ito, Len Yamaguchi, Hiroshi Konishi, Gen-ichi Nagasaki, Yukio Shiraki, Kentaro |
| description | Manipulation of enzyme activity at will presents various research and industrial applications. Herein we describe development of a technology for inactivation and reactivation of enzyme activities using a polyanionic poly(acrylic acid) (PAAc) and a polycationic poly(allylamine) (PAA). Enzyme activities of ribonuclease A (RNase A), lysozyme, cellulase, and alpha -amylase were lost through addition of PAAc or PAA because of their binding to the enzymes. The activity of these enzymes except for alpha -amylase was then fully restored from the complex by the addition of oppositely charged polymers. Such manipulation of enzyme activity using a complementary polymer pair system (CPPS) enables the expansion of biomedical and biotechnological applications of the enzymes, including realization of protein delivery and intelligent bioreactors. |
| doi_str_mv | 10.1039/c000930j |
| format | Article |
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| fulltext | fulltext |
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| ispartof | Soft matter, 2010-01, Vol.6 (21), p.5320-5326 |
| issn | 1744-683X 1744-6848 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1718963745 |
| source | Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Activation Binding Bioreactors Charging Enzyme activity Enzymes Ribonuclease A Switches |
| title | Enzyme switch by complementary polymer pair system (CPPS) |
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