Transmission mode desorption atmospheric pressure photoionization

Rationale Desorption atmospheric pressure photoionization (DAPPI) is an ambient mass spectrometry (MS) technique that is suitable for the direct analysis of polar and nonpolar compounds from a variety of surfaces. Conventional DAPPI uses reflection geometry, but here transmission mode (TM)‐DAPPI is introduced for fast and easy analysis of liquid samples. Methods Stainless steel and PEEK meshes were used as sampling support in TM‐DAPPI. The sample was applied either in the form of a droplet on the mesh, or by dipping the mesh in the sample solution. Physical parameters affecting the ionization efficiency were optimized for TM‐DAPPI. The mesh materials were used to extract compounds from aqueous samples, which were then analyzed by TM‐DAPPI. TM‐DAPPI and conventional DAPPI were compared. Results In TM‐DAPPI, intense signals for the analytes were achieved with less heating power, and lower nebulizer gas and dopant flow rates than optimally used in conventional DAPPI. Either due to this, or the different sample support material used, a much lower background and improved sensitivity compared to conventional DAPPI was achieved. The analytes could be extracted and concentrated from liquid samples on the mesh material used in TM‐DAPPI, which was especially efficient for the nonpolar benzo[a]pyrene. This effect was utilized in the analysis of triacylglycerols from cow milk. Conclusions While conventional DAPPI is still the method of choice for solid samples, TM‐DAPPI can be utilized as a fast, easily automated method for analyzing liquid samples. The mesh materials can be utilized for extraction of low polarity compounds, such as steroid hormones or PAHs from dilute, aqueous solutions, followed by subsequent analysis by TM‐DAPPI. Copyright © 2015 John Wiley & Sons, Ltd.