First detection of Leishmania spp. DNA in Brazilian bats captured strictly in urban areas
[Display omitted] •We analyzed 488 bats captured in urban area for the presence of Leishmania ssp. kDNA.•The 23.9% of bats were positive for the presence of Leishmania spp. kDNA.•We detected L. infantum and L. amazonensis kDNA in Desmodus rotundus bat.•Bats may be a potential reservoir of Leishmania...
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| Veröffentlicht in: | Acta tropica 2015-10, Vol.150, p.176-181 |
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| creator | Oliveira, Fernanda Müller de Costa, Luis Henrique Camargo Barros, Thainá Landim de Rauschkolb Katsuda Ito, Pier Kenji Colombo, Fábio Antonio Carvalho, Cristiano de Pedro, Wagner André Queiroz, Luzia Helena Nunes, Cáris Maroni |
| description | [Display omitted]
•We analyzed 488 bats captured in urban area for the presence of Leishmania ssp. kDNA.•The 23.9% of bats were positive for the presence of Leishmania spp. kDNA.•We detected L. infantum and L. amazonensis kDNA in Desmodus rotundus bat.•Bats may be a potential reservoir of Leishmania species in the urban scenario.
Leishmania spp. is a protozoan that maintains its life cycle in domestic and wild animals and it may include bats, a population that has increased in urban environments. This study aimed to investigate the presence of Leishmania spp. in bats captured strictly in urban areas that are endemic for visceral leishmaniasis. The spleen and skin samples of 488 bats from 21 endemic cities in northwestern São Paulo State, Brazil, were tested for the presence of Leishmania kDNA using real-time PCR. Differentiation from Trypanosoma spp. was achieved by amplifying a DNA fragment of the ribosomal RNA gene. The presence of Leishmania spp. kDNA was verified in 23.9% of bats and Trypanosoma spp. DNA was identified in 3.9%. Leishmania species differentiation revealed the presence of Leishmania amazonensis in 78.3% of the bats; L. infantum in 17.4%, and 1 sample (4.3%) showed a mix pattern of L. infantum and L. amazonensis. We also detected, for the first time, L. infantum and L. amazonensis DNA in Desmodus rotundus, the hematophagous bat. The presence of Leishmania spp. DNA in bats strictly from urban areas endemic for visceral leishmaniasis in the State of São Paulo, Brazil indicates that these wild and abundant animals are capable of harboring Leishmania spp. in this new scenario. Due to their longevity, high dispersion capacity and adaptability to synanthropic environments, they may play a role in the maintenance of the life cycle of Leishmania parasites. |
| doi_str_mv | 10.1016/j.actatropica.2015.07.010 |
| format | Article |
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•We analyzed 488 bats captured in urban area for the presence of Leishmania ssp. kDNA.•The 23.9% of bats were positive for the presence of Leishmania spp. kDNA.•We detected L. infantum and L. amazonensis kDNA in Desmodus rotundus bat.•Bats may be a potential reservoir of Leishmania species in the urban scenario.
Leishmania spp. is a protozoan that maintains its life cycle in domestic and wild animals and it may include bats, a population that has increased in urban environments. This study aimed to investigate the presence of Leishmania spp. in bats captured strictly in urban areas that are endemic for visceral leishmaniasis. The spleen and skin samples of 488 bats from 21 endemic cities in northwestern São Paulo State, Brazil, were tested for the presence of Leishmania kDNA using real-time PCR. Differentiation from Trypanosoma spp. was achieved by amplifying a DNA fragment of the ribosomal RNA gene. The presence of Leishmania spp. kDNA was verified in 23.9% of bats and Trypanosoma spp. DNA was identified in 3.9%. Leishmania species differentiation revealed the presence of Leishmania amazonensis in 78.3% of the bats; L. infantum in 17.4%, and 1 sample (4.3%) showed a mix pattern of L. infantum and L. amazonensis. We also detected, for the first time, L. infantum and L. amazonensis DNA in Desmodus rotundus, the hematophagous bat. The presence of Leishmania spp. DNA in bats strictly from urban areas endemic for visceral leishmaniasis in the State of São Paulo, Brazil indicates that these wild and abundant animals are capable of harboring Leishmania spp. in this new scenario. Due to their longevity, high dispersion capacity and adaptability to synanthropic environments, they may play a role in the maintenance of the life cycle of Leishmania parasites.</description><identifier>ISSN: 0001-706X</identifier><identifier>EISSN: 1873-6254</identifier><identifier>DOI: 10.1016/j.actatropica.2015.07.010</identifier><identifier>PMID: 26209107</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Animals, Wild - parasitology ; Bats ; Brazil - epidemiology ; Chiroptera - parasitology ; Disease Reservoirs ; Geography ; Leishmania - genetics ; Leishmania - isolation & purification ; Leishmaniasis ; Leishmaniasis, Visceral - epidemiology ; Leishmaniasis, Visceral - parasitology ; qPCR ; Real-Time Polymerase Chain Reaction ; Reservoir ; Trypanosoma ; Urban Population</subject><ispartof>Acta tropica, 2015-10, Vol.150, p.176-181</ispartof><rights>2015 Elsevier B.V.</rights><rights>Copyright © 2015 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c443t-ba2fdc5a6fa11336cc92ee66873d470a7db02bdf64889852cffb84a9548fa33d3</citedby><cites>FETCH-LOGICAL-c443t-ba2fdc5a6fa11336cc92ee66873d470a7db02bdf64889852cffb84a9548fa33d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0001706X15300590$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26209107$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Oliveira, Fernanda Müller de</creatorcontrib><creatorcontrib>Costa, Luis Henrique Camargo</creatorcontrib><creatorcontrib>Barros, Thainá Landim de</creatorcontrib><creatorcontrib>Rauschkolb Katsuda Ito, Pier Kenji</creatorcontrib><creatorcontrib>Colombo, Fábio Antonio</creatorcontrib><creatorcontrib>Carvalho, Cristiano de</creatorcontrib><creatorcontrib>Pedro, Wagner André</creatorcontrib><creatorcontrib>Queiroz, Luzia Helena</creatorcontrib><creatorcontrib>Nunes, Cáris Maroni</creatorcontrib><title>First detection of Leishmania spp. DNA in Brazilian bats captured strictly in urban areas</title><title>Acta tropica</title><addtitle>Acta Trop</addtitle><description>[Display omitted]
•We analyzed 488 bats captured in urban area for the presence of Leishmania ssp. kDNA.•The 23.9% of bats were positive for the presence of Leishmania spp. kDNA.•We detected L. infantum and L. amazonensis kDNA in Desmodus rotundus bat.•Bats may be a potential reservoir of Leishmania species in the urban scenario.
Leishmania spp. is a protozoan that maintains its life cycle in domestic and wild animals and it may include bats, a population that has increased in urban environments. This study aimed to investigate the presence of Leishmania spp. in bats captured strictly in urban areas that are endemic for visceral leishmaniasis. The spleen and skin samples of 488 bats from 21 endemic cities in northwestern São Paulo State, Brazil, were tested for the presence of Leishmania kDNA using real-time PCR. Differentiation from Trypanosoma spp. was achieved by amplifying a DNA fragment of the ribosomal RNA gene. The presence of Leishmania spp. kDNA was verified in 23.9% of bats and Trypanosoma spp. DNA was identified in 3.9%. Leishmania species differentiation revealed the presence of Leishmania amazonensis in 78.3% of the bats; L. infantum in 17.4%, and 1 sample (4.3%) showed a mix pattern of L. infantum and L. amazonensis. We also detected, for the first time, L. infantum and L. amazonensis DNA in Desmodus rotundus, the hematophagous bat. The presence of Leishmania spp. DNA in bats strictly from urban areas endemic for visceral leishmaniasis in the State of São Paulo, Brazil indicates that these wild and abundant animals are capable of harboring Leishmania spp. in this new scenario. Due to their longevity, high dispersion capacity and adaptability to synanthropic environments, they may play a role in the maintenance of the life cycle of Leishmania parasites.</description><subject>Animals</subject><subject>Animals, Wild - parasitology</subject><subject>Bats</subject><subject>Brazil - epidemiology</subject><subject>Chiroptera - parasitology</subject><subject>Disease Reservoirs</subject><subject>Geography</subject><subject>Leishmania - genetics</subject><subject>Leishmania - isolation & purification</subject><subject>Leishmaniasis</subject><subject>Leishmaniasis, Visceral - epidemiology</subject><subject>Leishmaniasis, Visceral - parasitology</subject><subject>qPCR</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Reservoir</subject><subject>Trypanosoma</subject><subject>Urban Population</subject><issn>0001-706X</issn><issn>1873-6254</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkE1r3DAQhkVJaDZp_0JRbrnYkWRbso_pNl-wNJcE2pMYSyOqxWu7khxIf320bFJ67GkY5pkZ3oeQc85Kzri83JZgEqQwzd5AKRhvSqZKxtkHsuKtqgopmvqIrBhjvFBM_jghpzFucydUIz6SEyEF6zhTK_LzxoeYqMWEJvlppJOjG_Tx1w5GDzTOc0m_fb-ifqRfA_zxg4eR9pAiNTCnJaClMQVv0vCyZ5bQ5zkEhPiJHDsYIn5-q2fk6eb6cX1XbB5u79dXm8LUdZWKHoSzpgHpgPOqksZ0AlHKHMPWioGyPRO9dbJu265thHGub2vomrp1UFW2OiMXh7tzmH4vGJPe-WhwGGDEaYmaK952vFK1yGh3QE2YYgzo9Bz8DsKL5kzvzeqt_ses3pvVTOlsNu9-eXuz9Du0fzffVWZgfQAwh332GHQ0HkeD1ofsVtvJ_8ebV_gdkIs</recordid><startdate>201510</startdate><enddate>201510</enddate><creator>Oliveira, Fernanda Müller de</creator><creator>Costa, Luis Henrique Camargo</creator><creator>Barros, Thainá Landim de</creator><creator>Rauschkolb Katsuda Ito, Pier Kenji</creator><creator>Colombo, Fábio Antonio</creator><creator>Carvalho, Cristiano de</creator><creator>Pedro, Wagner André</creator><creator>Queiroz, Luzia Helena</creator><creator>Nunes, Cáris Maroni</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201510</creationdate><title>First detection of Leishmania spp. DNA in Brazilian bats captured strictly in urban areas</title><author>Oliveira, Fernanda Müller de ; Costa, Luis Henrique Camargo ; Barros, Thainá Landim de ; Rauschkolb Katsuda Ito, Pier Kenji ; Colombo, Fábio Antonio ; Carvalho, Cristiano de ; Pedro, Wagner André ; Queiroz, Luzia Helena ; Nunes, Cáris Maroni</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c443t-ba2fdc5a6fa11336cc92ee66873d470a7db02bdf64889852cffb84a9548fa33d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Animals, Wild - parasitology</topic><topic>Bats</topic><topic>Brazil - epidemiology</topic><topic>Chiroptera - parasitology</topic><topic>Disease Reservoirs</topic><topic>Geography</topic><topic>Leishmania - genetics</topic><topic>Leishmania - isolation & purification</topic><topic>Leishmaniasis</topic><topic>Leishmaniasis, Visceral - epidemiology</topic><topic>Leishmaniasis, Visceral - parasitology</topic><topic>qPCR</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Reservoir</topic><topic>Trypanosoma</topic><topic>Urban Population</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Oliveira, Fernanda Müller de</creatorcontrib><creatorcontrib>Costa, Luis Henrique Camargo</creatorcontrib><creatorcontrib>Barros, Thainá Landim de</creatorcontrib><creatorcontrib>Rauschkolb Katsuda Ito, Pier Kenji</creatorcontrib><creatorcontrib>Colombo, Fábio Antonio</creatorcontrib><creatorcontrib>Carvalho, Cristiano de</creatorcontrib><creatorcontrib>Pedro, Wagner André</creatorcontrib><creatorcontrib>Queiroz, Luzia Helena</creatorcontrib><creatorcontrib>Nunes, Cáris Maroni</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Acta tropica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Oliveira, Fernanda Müller de</au><au>Costa, Luis Henrique Camargo</au><au>Barros, Thainá Landim de</au><au>Rauschkolb Katsuda Ito, Pier Kenji</au><au>Colombo, Fábio Antonio</au><au>Carvalho, Cristiano de</au><au>Pedro, Wagner André</au><au>Queiroz, Luzia Helena</au><au>Nunes, Cáris Maroni</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>First detection of Leishmania spp. DNA in Brazilian bats captured strictly in urban areas</atitle><jtitle>Acta tropica</jtitle><addtitle>Acta Trop</addtitle><date>2015-10</date><risdate>2015</risdate><volume>150</volume><spage>176</spage><epage>181</epage><pages>176-181</pages><issn>0001-706X</issn><eissn>1873-6254</eissn><abstract>[Display omitted]
•We analyzed 488 bats captured in urban area for the presence of Leishmania ssp. kDNA.•The 23.9% of bats were positive for the presence of Leishmania spp. kDNA.•We detected L. infantum and L. amazonensis kDNA in Desmodus rotundus bat.•Bats may be a potential reservoir of Leishmania species in the urban scenario.
Leishmania spp. is a protozoan that maintains its life cycle in domestic and wild animals and it may include bats, a population that has increased in urban environments. This study aimed to investigate the presence of Leishmania spp. in bats captured strictly in urban areas that are endemic for visceral leishmaniasis. The spleen and skin samples of 488 bats from 21 endemic cities in northwestern São Paulo State, Brazil, were tested for the presence of Leishmania kDNA using real-time PCR. Differentiation from Trypanosoma spp. was achieved by amplifying a DNA fragment of the ribosomal RNA gene. The presence of Leishmania spp. kDNA was verified in 23.9% of bats and Trypanosoma spp. DNA was identified in 3.9%. Leishmania species differentiation revealed the presence of Leishmania amazonensis in 78.3% of the bats; L. infantum in 17.4%, and 1 sample (4.3%) showed a mix pattern of L. infantum and L. amazonensis. We also detected, for the first time, L. infantum and L. amazonensis DNA in Desmodus rotundus, the hematophagous bat. The presence of Leishmania spp. DNA in bats strictly from urban areas endemic for visceral leishmaniasis in the State of São Paulo, Brazil indicates that these wild and abundant animals are capable of harboring Leishmania spp. in this new scenario. Due to their longevity, high dispersion capacity and adaptability to synanthropic environments, they may play a role in the maintenance of the life cycle of Leishmania parasites.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>26209107</pmid><doi>10.1016/j.actatropica.2015.07.010</doi><tpages>6</tpages></addata></record> |
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| ispartof | Acta tropica, 2015-10, Vol.150, p.176-181 |
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| subjects | Animals Animals, Wild - parasitology Bats Brazil - epidemiology Chiroptera - parasitology Disease Reservoirs Geography Leishmania - genetics Leishmania - isolation & purification Leishmaniasis Leishmaniasis, Visceral - epidemiology Leishmaniasis, Visceral - parasitology qPCR Real-Time Polymerase Chain Reaction Reservoir Trypanosoma Urban Population |
| title | First detection of Leishmania spp. DNA in Brazilian bats captured strictly in urban areas |
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