Effects of temperature and cellular interactions on the mechanics and morphology of human cancer cells investigated by atomic force microscopy
Cell mechanics plays an important role in cellular physiological activities. Recent studies have shown that cellular mechanical properties are novel biomarkers for indicating the cell states. In this article, temperature-controllable atomic force microscopy （AFM） was applied to quantitatively invest...
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| Veröffentlicht in: | Science China. Life sciences 2015-09, Vol.58 (9), p.889-901 |
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| creator | Li, Mi Liu, LianQing Xi, Ning Wang, YueChao Xiao, XiuBin Zhang, WeiJing |
| description | Cell mechanics plays an important role in cellular physiological activities. Recent studies have shown that cellular mechanical properties are novel biomarkers for indicating the cell states. In this article, temperature-controllable atomic force microscopy （AFM） was applied to quantitatively investigate the effects of temperature and cellular interactions on the mechanics and morphology of human cancer cells. First, AFM indenting experiments were performed on six types of human cells to investi- gate the changes of cellular Young＇s modulus at different temperatures and the results showed that the mechanical responses to the changes of temperature were variable for different types of cancer cells. Second, AFM imaging experiments were per- formed to observe the morphological changes in living cells at different temperatures and the results showed the significant changes of cell morphology caused by the alterations of temperature. Finally, by co-culturing human cancer cells with human immune cells, the mechanical and morphological changes in cancer cells were investigated. The results showed that the co-culture of cancer cells and immune cells could cause the distinct mechanical changes in cancer cells, but no significant morphological differences were observed. The experimental results improved our understanding of the effects of temperature and cellular interactions on the mechanics and morphology of cancer cells. |
| doi_str_mv | 10.1007/s11427-015-4914-4 |
| format | Article |
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Recent studies have shown that cellular mechanical properties are novel biomarkers for indicating the cell states. In this article, temperature-controllable atomic force microscopy （AFM） was applied to quantitatively investigate the effects of temperature and cellular interactions on the mechanics and morphology of human cancer cells. First, AFM indenting experiments were performed on six types of human cells to investi- gate the changes of cellular Young＇s modulus at different temperatures and the results showed that the mechanical responses to the changes of temperature were variable for different types of cancer cells. Second, AFM imaging experiments were per- formed to observe the morphological changes in living cells at different temperatures and the results showed the significant changes of cell morphology caused by the alterations of temperature. Finally, by co-culturing human cancer cells with human immune cells, the mechanical and morphological changes in cancer cells were investigated. The results showed that the co-culture of cancer cells and immune cells could cause the distinct mechanical changes in cancer cells, but no significant morphological differences were observed. The experimental results improved our understanding of the effects of temperature and cellular interactions on the mechanics and morphology of cancer cells.</description><identifier>ISSN: 1674-7305</identifier><identifier>EISSN: 1869-1889</identifier><identifier>DOI: 10.1007/s11427-015-4914-4</identifier><identifier>PMID: 26354505</identifier><language>eng</language><publisher>Beijing: Science China Press</publisher><subject>Biomarkers - metabolism ; Biomedical and Life Sciences ; Cell Adhesion ; Coculture Techniques ; Elastic Modulus ; HeLa Cells - cytology ; Humans ; Jurkat Cells - cytology ; Life Sciences ; MCF-7 Cells - cytology ; Microscopy, Atomic Force ; Neoplasms - metabolism ; Neoplasms - pathology ; Optics and Photonics ; Pressure ; Research Paper ; Temperature ; 压痕实验 ; 原子力显微镜 ; 机械性能 ; 温度变化 ; 癌细胞 ; 相互作用 ; 细胞力学 ; 细胞形态</subject><ispartof>Science China. Life sciences, 2015-09, Vol.58 (9), p.889-901</ispartof><rights>The Author(s) 2015. This article is published under license to BioMed Central Ltd. This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction</rights><rights>Science in China Press and Springer-Verlag GmbH 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c578t-fcb044002674a2c65f4ef331895ed7d2b0b200b55b92e956c6109f2024884e823</citedby><cites>FETCH-LOGICAL-c578t-fcb044002674a2c65f4ef331895ed7d2b0b200b55b92e956c6109f2024884e823</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/60112X/60112X.jpg</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11427-015-4914-4$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11427-015-4914-4$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26354505$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Mi</creatorcontrib><creatorcontrib>Liu, LianQing</creatorcontrib><creatorcontrib>Xi, Ning</creatorcontrib><creatorcontrib>Wang, YueChao</creatorcontrib><creatorcontrib>Xiao, XiuBin</creatorcontrib><creatorcontrib>Zhang, WeiJing</creatorcontrib><title>Effects of temperature and cellular interactions on the mechanics and morphology of human cancer cells investigated by atomic force microscopy</title><title>Science China. Life sciences</title><addtitle>Sci. China Life Sci</addtitle><addtitle>Sci China Life Sci</addtitle><description>Cell mechanics plays an important role in cellular physiological activities. Recent studies have shown that cellular mechanical properties are novel biomarkers for indicating the cell states. In this article, temperature-controllable atomic force microscopy （AFM） was applied to quantitatively investigate the effects of temperature and cellular interactions on the mechanics and morphology of human cancer cells. First, AFM indenting experiments were performed on six types of human cells to investi- gate the changes of cellular Young＇s modulus at different temperatures and the results showed that the mechanical responses to the changes of temperature were variable for different types of cancer cells. Second, AFM imaging experiments were per- formed to observe the morphological changes in living cells at different temperatures and the results showed the significant changes of cell morphology caused by the alterations of temperature. Finally, by co-culturing human cancer cells with human immune cells, the mechanical and morphological changes in cancer cells were investigated. The results showed that the co-culture of cancer cells and immune cells could cause the distinct mechanical changes in cancer cells, but no significant morphological differences were observed. The experimental results improved our understanding of the effects of temperature and cellular interactions on the mechanics and morphology of cancer cells.</description><subject>Biomarkers - metabolism</subject><subject>Biomedical and Life Sciences</subject><subject>Cell Adhesion</subject><subject>Coculture Techniques</subject><subject>Elastic Modulus</subject><subject>HeLa Cells - cytology</subject><subject>Humans</subject><subject>Jurkat Cells - cytology</subject><subject>Life Sciences</subject><subject>MCF-7 Cells - cytology</subject><subject>Microscopy, Atomic Force</subject><subject>Neoplasms - metabolism</subject><subject>Neoplasms - pathology</subject><subject>Optics and Photonics</subject><subject>Pressure</subject><subject>Research Paper</subject><subject>Temperature</subject><subject>压痕实验</subject><subject>原子力显微镜</subject><subject>机械性能</subject><subject>温度变化</subject><subject>癌细胞</subject><subject>相互作用</subject><subject>细胞力学</subject><subject>细胞形态</subject><issn>1674-7305</issn><issn>1869-1889</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kc1u1TAQRiNERau2D9ANsmDDJuB_O0tUFYpUiQ1dR44zvkmV2KntIN2X6DPj9N5WiAXe2LLPHM_oq6orgj8TjNWXRAinqsZE1LwhvOZvqjOiZVMTrZu35SwVrxXD4rS6TOkBl8UYpkq9q06pZIILLM6qpxvnwOaEgkMZ5gWiyWsEZHyPLEzTOpmIRp_Lvc1j8AX0KA-AZrCD8aNNz-gc4jKEKez2m2hYZ-ORNd5CfLakovgNKY87k6FH3R6ZHObRIheiLa7RxpBsWPYX1YkzU4LL435e3X-7-XV9W9_9_P7j-utdbYXSuXa2w5xjTMuMhlopHAfHGNGNgF71tMMdxbgTomsoNEJaSXDjKKZcaw6asvPq08G7xPC4ls7aeUxbp8ZDWFNLVHERyqgq6Md_0IewRl-626hGMEWELhQ5UNskKYJrlzjOJu5bgtstr_aQV1vyare8Wl5q3h_NazdD_1rxkk4B6AFI5cnvIP719X-sH46dDMHvHkvdq1hKSbHUxf8HDt-shQ</recordid><startdate>20150901</startdate><enddate>20150901</enddate><creator>Li, Mi</creator><creator>Liu, LianQing</creator><creator>Xi, Ning</creator><creator>Wang, YueChao</creator><creator>Xiao, XiuBin</creator><creator>Zhang, WeiJing</creator><general>Science China Press</general><general>Springer Nature B.V</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>~WA</scope><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7TK</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20150901</creationdate><title>Effects of temperature and cellular interactions on the mechanics and morphology of human cancer cells investigated by atomic force microscopy</title><author>Li, Mi ; 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Life sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Mi</au><au>Liu, LianQing</au><au>Xi, Ning</au><au>Wang, YueChao</au><au>Xiao, XiuBin</au><au>Zhang, WeiJing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of temperature and cellular interactions on the mechanics and morphology of human cancer cells investigated by atomic force microscopy</atitle><jtitle>Science China. Life sciences</jtitle><stitle>Sci. China Life Sci</stitle><addtitle>Sci China Life Sci</addtitle><date>2015-09-01</date><risdate>2015</risdate><volume>58</volume><issue>9</issue><spage>889</spage><epage>901</epage><pages>889-901</pages><issn>1674-7305</issn><eissn>1869-1889</eissn><abstract>Cell mechanics plays an important role in cellular physiological activities. Recent studies have shown that cellular mechanical properties are novel biomarkers for indicating the cell states. In this article, temperature-controllable atomic force microscopy （AFM） was applied to quantitatively investigate the effects of temperature and cellular interactions on the mechanics and morphology of human cancer cells. First, AFM indenting experiments were performed on six types of human cells to investi- gate the changes of cellular Young＇s modulus at different temperatures and the results showed that the mechanical responses to the changes of temperature were variable for different types of cancer cells. Second, AFM imaging experiments were per- formed to observe the morphological changes in living cells at different temperatures and the results showed the significant changes of cell morphology caused by the alterations of temperature. Finally, by co-culturing human cancer cells with human immune cells, the mechanical and morphological changes in cancer cells were investigated. The results showed that the co-culture of cancer cells and immune cells could cause the distinct mechanical changes in cancer cells, but no significant morphological differences were observed. The experimental results improved our understanding of the effects of temperature and cellular interactions on the mechanics and morphology of cancer cells.</abstract><cop>Beijing</cop><pub>Science China Press</pub><pmid>26354505</pmid><doi>10.1007/s11427-015-4914-4</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Biomarkers - metabolism Biomedical and Life Sciences Cell Adhesion Coculture Techniques Elastic Modulus HeLa Cells - cytology Humans Jurkat Cells - cytology Life Sciences MCF-7 Cells - cytology Microscopy, Atomic Force Neoplasms - metabolism Neoplasms - pathology Optics and Photonics Pressure Research Paper Temperature 压痕实验 原子力显微镜 机械性能 温度变化 癌细胞 相互作用 细胞力学 细胞形态 |
| title | Effects of temperature and cellular interactions on the mechanics and morphology of human cancer cells investigated by atomic force microscopy |
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