Relationship between Plasma Albumin Concentration and Plasma Volume in 5 Inbred Rat Strains
Using the Evans Blue procedure, we previously found strain-related differences in plasma volumes in 5 inbred rat strains. Because albumin binds strongly with Evans blue, this protein is important in the Evans blue method of plasma volume determination. Therefore, we speculated that interstrain diffe...
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| Veröffentlicht in: | Journal of the American Association for Laboratory Animal Science 2015-09, Vol.54 (5), p.459-464 |
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| creator | Rose, Rajiv Klemcke, Harold G |
| description | Using the Evans Blue procedure, we previously found strain-related differences in plasma volumes in 5 inbred rat strains. Because albumin binds strongly with Evans blue, this protein is important in the Evans blue method of plasma volume determination. Therefore, we speculated that
interstrain differences in plasma albumin concentration (PAC) could distort calculated plasma volumes. To address this concern, we used ELISA techniques to measure PAC in these inbred rat strains. In study A, the blood volume was measured by using Evans blue dye, and albumin was measured at
the start of hemorrhage. In study B, blood volume was not measured, and albumin was measured twice, near the start and end of hemorrhage (approximately 14 min apart). Neither study revealed any interstrain differences in PAC, which decreased after hemorrhage in all 5 strains. No correlation
was found between PAC and plasma volume, survival time, blood lactate, or blood base excess. Percentage changes in PAC during hemorrhage were greater in salt-sensitive compared with Lewis rats. Moreover, these percentage changes were associated with survival time in Fawn hooded hypertensive
rats. Our data show that the plasma volumes we measured previously were not misrepresented due to variations in PAC. |
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interstrain differences in plasma albumin concentration (PAC) could distort calculated plasma volumes. To address this concern, we used ELISA techniques to measure PAC in these inbred rat strains. In study A, the blood volume was measured by using Evans blue dye, and albumin was measured at
the start of hemorrhage. In study B, blood volume was not measured, and albumin was measured twice, near the start and end of hemorrhage (approximately 14 min apart). Neither study revealed any interstrain differences in PAC, which decreased after hemorrhage in all 5 strains. No correlation
was found between PAC and plasma volume, survival time, blood lactate, or blood base excess. Percentage changes in PAC during hemorrhage were greater in salt-sensitive compared with Lewis rats. Moreover, these percentage changes were associated with survival time in Fawn hooded hypertensive
rats. Our data show that the plasma volumes we measured previously were not misrepresented due to variations in PAC.</description><identifier>ISSN: 1559-6109</identifier><identifier>PMID: 26424242</identifier><language>eng</language><publisher>United States: American Association for Laboratory Animal Science</publisher><subject>Animals ; Biology ; Blood Volume ; Evans Blue - analysis ; Hemorrhage - physiopathology ; Hemorrhage - veterinary ; Male ; Plasma Volume ; Rats ; Rats, Inbred Strains - blood ; Rats, Inbred Strains - classification ; Rats, Inbred Strains - physiology ; Serum Albumin - analysis ; Species Specificity</subject><ispartof>Journal of the American Association for Laboratory Animal Science, 2015-09, Vol.54 (5), p.459-464</ispartof><rights>American Association for Laboratory Animal Science 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4587612/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4587612/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,288,314,724,777,781,882,53772,53774</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26424242$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rose, Rajiv</creatorcontrib><creatorcontrib>Klemcke, Harold G</creatorcontrib><title>Relationship between Plasma Albumin Concentration and Plasma Volume in 5 Inbred Rat Strains</title><title>Journal of the American Association for Laboratory Animal Science</title><addtitle>J Am Assoc Lab Animal Sci</addtitle><addtitle>J Am Assoc Lab Anim Sci</addtitle><description>Using the Evans Blue procedure, we previously found strain-related differences in plasma volumes in 5 inbred rat strains. Because albumin binds strongly with Evans blue, this protein is important in the Evans blue method of plasma volume determination. Therefore, we speculated that
interstrain differences in plasma albumin concentration (PAC) could distort calculated plasma volumes. To address this concern, we used ELISA techniques to measure PAC in these inbred rat strains. In study A, the blood volume was measured by using Evans blue dye, and albumin was measured at
the start of hemorrhage. In study B, blood volume was not measured, and albumin was measured twice, near the start and end of hemorrhage (approximately 14 min apart). Neither study revealed any interstrain differences in PAC, which decreased after hemorrhage in all 5 strains. No correlation
was found between PAC and plasma volume, survival time, blood lactate, or blood base excess. Percentage changes in PAC during hemorrhage were greater in salt-sensitive compared with Lewis rats. Moreover, these percentage changes were associated with survival time in Fawn hooded hypertensive
rats. Our data show that the plasma volumes we measured previously were not misrepresented due to variations in PAC.</description><subject>Animals</subject><subject>Biology</subject><subject>Blood Volume</subject><subject>Evans Blue - analysis</subject><subject>Hemorrhage - physiopathology</subject><subject>Hemorrhage - veterinary</subject><subject>Male</subject><subject>Plasma Volume</subject><subject>Rats</subject><subject>Rats, Inbred Strains - blood</subject><subject>Rats, Inbred Strains - classification</subject><subject>Rats, Inbred Strains - physiology</subject><subject>Serum Albumin - analysis</subject><subject>Species Specificity</subject><issn>1559-6109</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kU1v3CAQhn1olaRp_0LFsZeVGGxgfakUbb8iRUqVNL30MBoMu2GF8dbgRM2vD85uqvYQODASj54XZl5VJyBlu1DA2-PqTUpbzqWWtTiqjoVqxLxPql9XLlD2Q0y3fseMy_fORfY9UOqJnQUz9T6y1RA7F_P4BDKK9hn4OYSpd6wgkp1HMzrLriiz64L6mN5Wr9cUknt3OE-rmy-ff6y-LS4uv56vzi4Wvm7bvFACwFjqlFDSys5qvm7qDmqjVGs7x42otRFaN45Id1y72pLiTeNaw4WVqj6tPu69u8n0zu6fGnA3-p7GPziQx_9vor_FzXCHjVxqBaIIPhwE4_B7cilj71PnQqDohikhaFi2wIWGgr7_N-tvyHNHC_BpD_i4KXGE22EaY_k-EpWm4XZ_CA4S-dOSzaHgEmnMczHnXL6g8d3BNE93Hi7eySbKYhTAl6ARQAJat6YpZMw04uYBE9SPthOivg</recordid><startdate>20150901</startdate><enddate>20150901</enddate><creator>Rose, Rajiv</creator><creator>Klemcke, Harold G</creator><general>American Association for Laboratory Animal Science</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20150901</creationdate><title>Relationship between Plasma Albumin Concentration and Plasma Volume in 5 Inbred Rat Strains</title><author>Rose, Rajiv ; Klemcke, Harold G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i399t-6211bdac6265d5cd70f43c13b669dce0b237b2774eaa7c07e3da6044e9b02d563</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Biology</topic><topic>Blood Volume</topic><topic>Evans Blue - analysis</topic><topic>Hemorrhage - physiopathology</topic><topic>Hemorrhage - veterinary</topic><topic>Male</topic><topic>Plasma Volume</topic><topic>Rats</topic><topic>Rats, Inbred Strains - blood</topic><topic>Rats, Inbred Strains - classification</topic><topic>Rats, Inbred Strains - physiology</topic><topic>Serum Albumin - analysis</topic><topic>Species Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rose, Rajiv</creatorcontrib><creatorcontrib>Klemcke, Harold G</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of the American Association for Laboratory Animal Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rose, Rajiv</au><au>Klemcke, Harold G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Relationship between Plasma Albumin Concentration and Plasma Volume in 5 Inbred Rat Strains</atitle><jtitle>Journal of the American Association for Laboratory Animal Science</jtitle><stitle>J Am Assoc Lab Animal Sci</stitle><addtitle>J Am Assoc Lab Anim Sci</addtitle><date>2015-09-01</date><risdate>2015</risdate><volume>54</volume><issue>5</issue><spage>459</spage><epage>464</epage><pages>459-464</pages><issn>1559-6109</issn><abstract>Using the Evans Blue procedure, we previously found strain-related differences in plasma volumes in 5 inbred rat strains. Because albumin binds strongly with Evans blue, this protein is important in the Evans blue method of plasma volume determination. Therefore, we speculated that
interstrain differences in plasma albumin concentration (PAC) could distort calculated plasma volumes. To address this concern, we used ELISA techniques to measure PAC in these inbred rat strains. In study A, the blood volume was measured by using Evans blue dye, and albumin was measured at
the start of hemorrhage. In study B, blood volume was not measured, and albumin was measured twice, near the start and end of hemorrhage (approximately 14 min apart). Neither study revealed any interstrain differences in PAC, which decreased after hemorrhage in all 5 strains. No correlation
was found between PAC and plasma volume, survival time, blood lactate, or blood base excess. Percentage changes in PAC during hemorrhage were greater in salt-sensitive compared with Lewis rats. Moreover, these percentage changes were associated with survival time in Fawn hooded hypertensive
rats. Our data show that the plasma volumes we measured previously were not misrepresented due to variations in PAC.</abstract><cop>United States</cop><pub>American Association for Laboratory Animal Science</pub><pmid>26424242</pmid><tpages>6</tpages></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; IngentaConnect Free/Open Access Journals; PubMed Central |
| subjects | Animals Biology Blood Volume Evans Blue - analysis Hemorrhage - physiopathology Hemorrhage - veterinary Male Plasma Volume Rats Rats, Inbred Strains - blood Rats, Inbred Strains - classification Rats, Inbred Strains - physiology Serum Albumin - analysis Species Specificity |
| title | Relationship between Plasma Albumin Concentration and Plasma Volume in 5 Inbred Rat Strains |
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