A novel electrochemical biosensor for selective determination of mercury ions based on DNA hybridization
An electrochemical biosensor was developed for Hg2+ determination based on DNA hybridization. In the presence of Hg2+, the target and probe DNAs with thymine–thymine (T–T) mismatches could hybridize by forming T–Hg2+–T complex. This induced DNA hybridization led to the decrease in reduction peak cur...
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| Veröffentlicht in: | Analytical biochemistry 2015-11, Vol.488, p.12-13 |
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| container_title | Analytical biochemistry |
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| creator | Ebrahimi, Maryam Raoof, Jahan Bakhsh Ojani, Reza Bagheryan, Zahra |
| description | An electrochemical biosensor was developed for Hg2+ determination based on DNA hybridization. In the presence of Hg2+, the target and probe DNAs with thymine–thymine (T–T) mismatches could hybridize by forming T–Hg2+–T complex. This induced DNA hybridization led to the decrease in reduction peak currents of ethyl green (EG) as electroactive label, which could be used for determination of Hg2+. The difference in the value of the peak currents of EG before and after DNA hybridization (ΔI) was linear with the concentration of Hg2+ in the range of 9.0 × 10−11–1.0 × 10−9 M. The detection limit was 3.08 × 10−11 M. |
| doi_str_mv | 10.1016/j.ab.2015.07.011 |
| format | Article |
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In the presence of Hg2+, the target and probe DNAs with thymine–thymine (T–T) mismatches could hybridize by forming T–Hg2+–T complex. This induced DNA hybridization led to the decrease in reduction peak currents of ethyl green (EG) as electroactive label, which could be used for determination of Hg2+. The difference in the value of the peak currents of EG before and after DNA hybridization (ΔI) was linear with the concentration of Hg2+ in the range of 9.0 × 10−11–1.0 × 10−9 M. The detection limit was 3.08 × 10−11 M.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/j.ab.2015.07.011</identifier><identifier>PMID: 26255700</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Base Pair Mismatch - drug effects ; Biosensing Techniques ; Coloring Agents - chemistry ; DNA - chemistry ; DNA hybridization ; DNA, Single-Stranded - chemistry ; Electrochemical Techniques ; Ethyl green ; Iran ; Mercury - analysis ; Mercury - pharmacology ; Mercury ions biosensor ; Nucleic Acid Hybridization - drug effects ; Oligonucleotides - chemistry ; Osmolar Concentration ; Poly T - chemistry ; Quaternary Ammonium Compounds - chemistry ; Silver nanoparticles ; Static Electricity ; T–Hg2+–T complex ; Water Pollutants, Chemical - analysis ; Water Pollutants, Chemical - pharmacology</subject><ispartof>Analytical biochemistry, 2015-11, Vol.488, p.12-13</ispartof><rights>2015 Elsevier Inc.</rights><rights>Copyright © 2015 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c350t-232f93d91a4c2b597fd989dcb3beef60a2ab7f11ec2c9a944d510687843d53dd3</citedby><cites>FETCH-LOGICAL-c350t-232f93d91a4c2b597fd989dcb3beef60a2ab7f11ec2c9a944d510687843d53dd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0003269715003619$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26255700$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ebrahimi, Maryam</creatorcontrib><creatorcontrib>Raoof, Jahan Bakhsh</creatorcontrib><creatorcontrib>Ojani, Reza</creatorcontrib><creatorcontrib>Bagheryan, Zahra</creatorcontrib><title>A novel electrochemical biosensor for selective determination of mercury ions based on DNA hybridization</title><title>Analytical biochemistry</title><addtitle>Anal Biochem</addtitle><description>An electrochemical biosensor was developed for Hg2+ determination based on DNA hybridization. In the presence of Hg2+, the target and probe DNAs with thymine–thymine (T–T) mismatches could hybridize by forming T–Hg2+–T complex. This induced DNA hybridization led to the decrease in reduction peak currents of ethyl green (EG) as electroactive label, which could be used for determination of Hg2+. The difference in the value of the peak currents of EG before and after DNA hybridization (ΔI) was linear with the concentration of Hg2+ in the range of 9.0 × 10−11–1.0 × 10−9 M. The detection limit was 3.08 × 10−11 M.</description><subject>Base Pair Mismatch - drug effects</subject><subject>Biosensing Techniques</subject><subject>Coloring Agents - chemistry</subject><subject>DNA - chemistry</subject><subject>DNA hybridization</subject><subject>DNA, Single-Stranded - chemistry</subject><subject>Electrochemical Techniques</subject><subject>Ethyl green</subject><subject>Iran</subject><subject>Mercury - analysis</subject><subject>Mercury - pharmacology</subject><subject>Mercury ions biosensor</subject><subject>Nucleic Acid Hybridization - drug effects</subject><subject>Oligonucleotides - chemistry</subject><subject>Osmolar Concentration</subject><subject>Poly T - chemistry</subject><subject>Quaternary Ammonium Compounds - chemistry</subject><subject>Silver nanoparticles</subject><subject>Static Electricity</subject><subject>T–Hg2+–T complex</subject><subject>Water Pollutants, Chemical - analysis</subject><subject>Water Pollutants, Chemical - pharmacology</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kMtrGzEQh0VJaJy0956Cjr3sdiTtS70ZNy8w6aU9Cz1msczuypHWBvevrxK7veUwDMN884P5CPnCoGTAmm_bUpuSA6tLaEtg7ANZMJBNAQLkBVkAgCh4I9srcp3SFjJR1c1HcsUbXtctwIJslnQKBxwoDmjnGOwGR2_1QI0PCacUIu1zpbe1PyB1OGMc_aRnHyYaejpitPt4pHlM1OiEjubFj-cl3RxN9M7_eUM_kcteDwk_n_sN-X1_92v1WKx_PjytluvCihrmggveS-Ek05XlppZt72QnnTXCIPYNaK5N2zOGllupZVW5mkHTtV0lXC2cEzfk6yl3F8PLHtOsRp8sDoOeMOyTYi3rJFSdqDIKJ9TGkFLEXu2iH3U8Kgbq1a_aKm3Uq18Frcr28sntOX1vRnT_D_4JzcD3E4D5x4PHqJL1OFl0PmaDygX_fvpfT5WLmQ</recordid><startdate>20151101</startdate><enddate>20151101</enddate><creator>Ebrahimi, Maryam</creator><creator>Raoof, Jahan Bakhsh</creator><creator>Ojani, Reza</creator><creator>Bagheryan, Zahra</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20151101</creationdate><title>A novel electrochemical biosensor for selective determination of mercury ions based on DNA hybridization</title><author>Ebrahimi, Maryam ; Raoof, Jahan Bakhsh ; Ojani, Reza ; Bagheryan, Zahra</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c350t-232f93d91a4c2b597fd989dcb3beef60a2ab7f11ec2c9a944d510687843d53dd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Base Pair Mismatch - drug effects</topic><topic>Biosensing Techniques</topic><topic>Coloring Agents - chemistry</topic><topic>DNA - chemistry</topic><topic>DNA hybridization</topic><topic>DNA, Single-Stranded - chemistry</topic><topic>Electrochemical Techniques</topic><topic>Ethyl green</topic><topic>Iran</topic><topic>Mercury - analysis</topic><topic>Mercury - pharmacology</topic><topic>Mercury ions biosensor</topic><topic>Nucleic Acid Hybridization - drug effects</topic><topic>Oligonucleotides - chemistry</topic><topic>Osmolar Concentration</topic><topic>Poly T - chemistry</topic><topic>Quaternary Ammonium Compounds - chemistry</topic><topic>Silver nanoparticles</topic><topic>Static Electricity</topic><topic>T–Hg2+–T complex</topic><topic>Water Pollutants, Chemical - analysis</topic><topic>Water Pollutants, Chemical - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ebrahimi, Maryam</creatorcontrib><creatorcontrib>Raoof, Jahan Bakhsh</creatorcontrib><creatorcontrib>Ojani, Reza</creatorcontrib><creatorcontrib>Bagheryan, Zahra</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ebrahimi, Maryam</au><au>Raoof, Jahan Bakhsh</au><au>Ojani, Reza</au><au>Bagheryan, Zahra</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A novel electrochemical biosensor for selective determination of mercury ions based on DNA hybridization</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>2015-11-01</date><risdate>2015</risdate><volume>488</volume><spage>12</spage><epage>13</epage><pages>12-13</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>An electrochemical biosensor was developed for Hg2+ determination based on DNA hybridization. In the presence of Hg2+, the target and probe DNAs with thymine–thymine (T–T) mismatches could hybridize by forming T–Hg2+–T complex. This induced DNA hybridization led to the decrease in reduction peak currents of ethyl green (EG) as electroactive label, which could be used for determination of Hg2+. The difference in the value of the peak currents of EG before and after DNA hybridization (ΔI) was linear with the concentration of Hg2+ in the range of 9.0 × 10−11–1.0 × 10−9 M. The detection limit was 3.08 × 10−11 M.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>26255700</pmid><doi>10.1016/j.ab.2015.07.011</doi><tpages>2</tpages></addata></record> |
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| subjects | Base Pair Mismatch - drug effects Biosensing Techniques Coloring Agents - chemistry DNA - chemistry DNA hybridization DNA, Single-Stranded - chemistry Electrochemical Techniques Ethyl green Iran Mercury - analysis Mercury - pharmacology Mercury ions biosensor Nucleic Acid Hybridization - drug effects Oligonucleotides - chemistry Osmolar Concentration Poly T - chemistry Quaternary Ammonium Compounds - chemistry Silver nanoparticles Static Electricity T–Hg2+–T complex Water Pollutants, Chemical - analysis Water Pollutants, Chemical - pharmacology |
| title | A novel electrochemical biosensor for selective determination of mercury ions based on DNA hybridization |
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