Toward refinement of the colony-forming unit-granulocyte/macrophage clonogenic assay: Inclusion of a metabolic system
This work represents a first attempt to refine the colony-forming unit-granulocyte/macrophage (CFU-GM) clonogenic assay by incorporating liver microsomes and co-factors as a metabolic system into the in vitro test system in response to an ECVAM recommendation [Gribaldo, L., Bueren, J., Deldar, A., H...
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| Veröffentlicht in: | Toxicology (Amsterdam) 2006-08, Vol.20 (5), p.743-749 |
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| creator | Dal Negro, Gianni Vandin, Luca Bonato, Monica Sciuscio, Davide |
| description | This work represents a first attempt to refine the colony-forming unit-granulocyte/macrophage (CFU-GM) clonogenic assay by incorporating liver microsomes and co-factors as a metabolic system into the in vitro test system in response to an ECVAM recommendation [Gribaldo, L., Bueren, J., Deldar, A., Hokland, P., Meredith, C., Moneta, D., Mossesso, P., Parchment, R., Parent-Massin, D., Pessina, A., San Roman, J., Schoeters, G., 1996. The use of in vitro systems for evaluating haematotoxicity. ECVAM Workshop Report 14. ATLA 24, 211–231]. From the comparison of results obtained with the CFU-GM clonogenic assay currently used and with the new experimental protocol, different toxicity on granulocyte/macrophage precursors was demonstrated, when drugs with a known metabolism in vivo were tested. |
| doi_str_mv | 10.1016/j.tiv.2005.10.016 |
| format | Article |
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The use of in vitro systems for evaluating haematotoxicity. ECVAM Workshop Report 14. ATLA 24, 211–231]. From the comparison of results obtained with the CFU-GM clonogenic assay currently used and with the new experimental protocol, different toxicity on granulocyte/macrophage precursors was demonstrated, when drugs with a known metabolism in vivo were tested.</description><identifier>ISSN: 0887-2333</identifier><identifier>ISSN: 0300-483X</identifier><identifier>EISSN: 1879-3177</identifier><identifier>DOI: 10.1016/j.tiv.2005.10.016</identifier><identifier>PMID: 16324821</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; Bone marrow ; Cefazolin - toxicity ; Cefotaxime - toxicity ; Ciprofloxacin - toxicity ; Colony-forming unit-granulocyte/macrophages ; Colony-Forming Units Assay - methods ; Cytochrome P450 ; Doxorubicin - toxicity ; Drug metabolism ; Granulocytes - drug effects ; Granulocytes - physiology ; Hematopoietic Stem Cells - drug effects ; Macrophages - drug effects ; Macrophages - physiology ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Inbred DBA ; Microsomes, Liver - metabolism ; Rats ; Rats, Sprague-Dawley ; Valproic Acid - toxicity ; Zidovudine - toxicity</subject><ispartof>Toxicology (Amsterdam), 2006-08, Vol.20 (5), p.743-749</ispartof><rights>2005 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c382t-9790d08796915010ccbfa83e795308650bc53232a9c2570d5a1b777c9d5e7d323</citedby><cites>FETCH-LOGICAL-c382t-9790d08796915010ccbfa83e795308650bc53232a9c2570d5a1b777c9d5e7d323</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0887233305002298$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16324821$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dal Negro, Gianni</creatorcontrib><creatorcontrib>Vandin, Luca</creatorcontrib><creatorcontrib>Bonato, Monica</creatorcontrib><creatorcontrib>Sciuscio, Davide</creatorcontrib><title>Toward refinement of the colony-forming unit-granulocyte/macrophage clonogenic assay: Inclusion of a metabolic system</title><title>Toxicology (Amsterdam)</title><addtitle>Toxicol In Vitro</addtitle><description>This work represents a first attempt to refine the colony-forming unit-granulocyte/macrophage (CFU-GM) clonogenic assay by incorporating liver microsomes and co-factors as a metabolic system into the in vitro test system in response to an ECVAM recommendation [Gribaldo, L., Bueren, J., Deldar, A., Hokland, P., Meredith, C., Moneta, D., Mossesso, P., Parchment, R., Parent-Massin, D., Pessina, A., San Roman, J., Schoeters, G., 1996. The use of in vitro systems for evaluating haematotoxicity. ECVAM Workshop Report 14. ATLA 24, 211–231]. From the comparison of results obtained with the CFU-GM clonogenic assay currently used and with the new experimental protocol, different toxicity on granulocyte/macrophage precursors was demonstrated, when drugs with a known metabolism in vivo were tested.</description><subject>Animals</subject><subject>Bone marrow</subject><subject>Cefazolin - toxicity</subject><subject>Cefotaxime - toxicity</subject><subject>Ciprofloxacin - toxicity</subject><subject>Colony-forming unit-granulocyte/macrophages</subject><subject>Colony-Forming Units Assay - methods</subject><subject>Cytochrome P450</subject><subject>Doxorubicin - toxicity</subject><subject>Drug metabolism</subject><subject>Granulocytes - drug effects</subject><subject>Granulocytes - physiology</subject><subject>Hematopoietic Stem Cells - drug effects</subject><subject>Macrophages - drug effects</subject><subject>Macrophages - physiology</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Inbred DBA</subject><subject>Microsomes, Liver - metabolism</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Valproic Acid - toxicity</subject><subject>Zidovudine - toxicity</subject><issn>0887-2333</issn><issn>0300-483X</issn><issn>1879-3177</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtOwzAQRS0EouXxAWxQVuzS-kHqBFYI8ZKQ2MDacpxJ6yqxi-0U5e-ZqpXYsbI8c-ZK9xByxeiMUbaYr2fJbmec0gL_M5wckSkrZZULJuUxmdKylDkXQkzIWYxrimDJ6SmZsIXgtyVnUzJ8-h8dmixAax304FLm2yytIDO-827MWx9665bZ4GzKl0G7ofNmTDDvtQl-s9JLRJH0S3DWZDpGPd5lb850Q7Te7dJ01kPSte9wH8eYoL8gJ63uIlwe3nPy9fz0-fiav3-8vD0-vOdGlDzllaxoQ7HQomIFZdSYutWlAFkVgpaLgtamEFxwXRleSNoUmtVSSlM1BcgGN-fkZp-7Cf57gJhUb6OBrtMO_BAVk6jrVjAE2R7ETjGiDLUJttdhVIyqnWu1Vuha7VzvRjjBm-tD-FD30PxdHOQicL8HACtuLQQVjQVnoLEBTFKNt__E_wJhlJDB</recordid><startdate>20060801</startdate><enddate>20060801</enddate><creator>Dal Negro, Gianni</creator><creator>Vandin, Luca</creator><creator>Bonato, Monica</creator><creator>Sciuscio, Davide</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>20060801</creationdate><title>Toward refinement of the colony-forming unit-granulocyte/macrophage clonogenic assay: Inclusion of a metabolic system</title><author>Dal Negro, Gianni ; Vandin, Luca ; Bonato, Monica ; Sciuscio, Davide</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c382t-9790d08796915010ccbfa83e795308650bc53232a9c2570d5a1b777c9d5e7d323</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Bone marrow</topic><topic>Cefazolin - toxicity</topic><topic>Cefotaxime - toxicity</topic><topic>Ciprofloxacin - toxicity</topic><topic>Colony-forming unit-granulocyte/macrophages</topic><topic>Colony-Forming Units Assay - methods</topic><topic>Cytochrome P450</topic><topic>Doxorubicin - toxicity</topic><topic>Drug metabolism</topic><topic>Granulocytes - drug effects</topic><topic>Granulocytes - physiology</topic><topic>Hematopoietic Stem Cells - drug effects</topic><topic>Macrophages - drug effects</topic><topic>Macrophages - physiology</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Inbred DBA</topic><topic>Microsomes, Liver - metabolism</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Valproic Acid - toxicity</topic><topic>Zidovudine - toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dal Negro, Gianni</creatorcontrib><creatorcontrib>Vandin, Luca</creatorcontrib><creatorcontrib>Bonato, Monica</creatorcontrib><creatorcontrib>Sciuscio, Davide</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Toxicology (Amsterdam)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dal Negro, Gianni</au><au>Vandin, Luca</au><au>Bonato, Monica</au><au>Sciuscio, Davide</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Toward refinement of the colony-forming unit-granulocyte/macrophage clonogenic assay: Inclusion of a metabolic system</atitle><jtitle>Toxicology (Amsterdam)</jtitle><addtitle>Toxicol In Vitro</addtitle><date>2006-08-01</date><risdate>2006</risdate><volume>20</volume><issue>5</issue><spage>743</spage><epage>749</epage><pages>743-749</pages><issn>0887-2333</issn><issn>0300-483X</issn><eissn>1879-3177</eissn><abstract>This work represents a first attempt to refine the colony-forming unit-granulocyte/macrophage (CFU-GM) clonogenic assay by incorporating liver microsomes and co-factors as a metabolic system into the in vitro test system in response to an ECVAM recommendation [Gribaldo, L., Bueren, J., Deldar, A., Hokland, P., Meredith, C., Moneta, D., Mossesso, P., Parchment, R., Parent-Massin, D., Pessina, A., San Roman, J., Schoeters, G., 1996. 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| ispartof | Toxicology (Amsterdam), 2006-08, Vol.20 (5), p.743-749 |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Bone marrow Cefazolin - toxicity Cefotaxime - toxicity Ciprofloxacin - toxicity Colony-forming unit-granulocyte/macrophages Colony-Forming Units Assay - methods Cytochrome P450 Doxorubicin - toxicity Drug metabolism Granulocytes - drug effects Granulocytes - physiology Hematopoietic Stem Cells - drug effects Macrophages - drug effects Macrophages - physiology Male Mice Mice, Inbred C57BL Mice, Inbred DBA Microsomes, Liver - metabolism Rats Rats, Sprague-Dawley Valproic Acid - toxicity Zidovudine - toxicity |
| title | Toward refinement of the colony-forming unit-granulocyte/macrophage clonogenic assay: Inclusion of a metabolic system |
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