Identification of Tissue-Restricted Bioreaction Suitable for in Vivo Targeting by Fluorescent Substrate Library-Based Enzyme Discovery

Tissue-restricted bioreactions can be utilized to design chemical-biological tools and prodrugs. We have developed a fluorescent-substrate-library-based enzyme discovery approach to screen tissue extracts for enzymatic activities of interest. Assay-positive candidate proteins were identified by dice...

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Veröffentlicht in:	Journal of the American Chemical Society 2015-09, Vol.137 (38), p.12187-12190
Hauptverfasser:	Yoshioka, Kentaro, Komatsu, Toru, Nakada, Akihiro, Onagi, Jun, Kuriki, Yugo, Kawaguchi, Mitsuyasu, Terai, Takuya, Ueno, Tasuku, Hanaoka, Kenjiro, Nagano, Tetsuo, Urano, Yasuteru
Format:	Artikel
Sprache:	eng
Schlagworte:	Anilides - chemistry Anilides - metabolism Carboxylesterase - metabolism Coumarins - chemistry Coumarins - metabolism Enzyme Activation Fluorescence Humans Kidney - enzymology Kidney - metabolism Liver - enzymology Liver - metabolism Substrate Specificity
Online-Zugang:	Volltext
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