A morphometric analysis of glomerular and tubular alterations following fast-neutron irradiation of the pig and monkey kidney
Purpose: The morphologic responses of the pig and monkey kidney to fractionated fast-neutron irradiation were assessed. Methods and Materials: The right kidney of approximately 14-week-old female Large White pigs was irradiated with 6.6–12.2 Gy of fast neutrons (42 MeV d→Be) given as 12 fractions ov...
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| Veröffentlicht in: | International journal of radiation oncology, biology, physics biology, physics, 1998-07, Vol.41 (5), p.1149-1156 |
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| creator | Robbins, Mike E.C Stephens, L.Clifton Johnston, Dennis A Thames, Howard D Peters, Lester J Hopewell, John W Ang, K.Kian |
| description | Purpose: The morphologic responses of the pig and monkey kidney to fractionated fast-neutron irradiation were assessed.
Methods and Materials: The right kidney of approximately 14-week-old female Large White pigs was irradiated with 6.6–12.2 Gy of fast neutrons (42 MeV
d→Be) given as 12 fractions over 18 days; the left kidney served as the contralateral unirradiated kidney. Both kidneys were removed at necropsy 2 years postirradiation. In addition, the remaining hypertrophied kidney of four unilaterally nephrectomized adult rhesus monkeys was irradiated with a total dose of 11.0 Gy fast neutrons (45 MeV
p→Be) given in an identical fractionation regimen to that used in the pig studies. These kidneys were removed when the animals exhibited renal failure, between 32–94 weeks postirradiation. Glomeruli were assessed for the presence of pathologic features, including intercapillary eosinophilic material (ICE), ectatic capillaries, thrombi, hemorrhage, and sclerosis. The relative proportion of renal cortex occupied by glomeruli, interstitium, normal, or abnormal tubules was determined using a Chalkley point grid.
Results: The incidence of normal glomeruli, ectatic capillaries, thrombosis, and periglomerular fibrosis were significantly different in the irradiated pig kidneys compared with the unirradiated contralateral kidneys (
p ≤ 0.02). Linear regression analysis demonstrated a significant dose relationship in terms of normal glomeruli, ectatic capillaries, and ICE (
r ≥ 0.64;
p ≤ 0.04). Irradiation was also associated with a significant (
p < 0.0001) decrease and increase in the volume of renal cortex occupied by normal and abnormal tubules, respectively. Similar morphometric changes were noted in the irradiated monkey kidneys.
Conclusions: The morphologic changes seen in the pig and monkey kidney after fractionated irradiation with fast neutrons are similar to those previously noted after single-dose or fractionated-photon irradiation. These findings support the hypothesis that the development of radiation nephropathy in these various models involves common pathophysiological mechanisms. |
| doi_str_mv | 10.1016/S0360-3016(98)00161-8 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_17183307</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0360301698001618</els_id><sourcerecordid>17183307</sourcerecordid><originalsourceid>FETCH-LOGICAL-c420t-5183d3e8989a5439ba1ab76db7bcc71534384939cc6dc2c9daa4a621103b646e3</originalsourceid><addsrcrecordid>eNqFkUtvFDEMgCMEKkvhJ1TKASE4DCSTeeWEqoqXVKmHgsQt8iSebWgmWZJM0R7635t9aK-cbNmf7egLIRecfeSMd59umehYJUr6Xg4fWIm8Gp6RFR96WYm2_f2crE7IS_IqpT-sULxvzsiZ7Lnkdbcij5d0DnFzF2bM0WoKHtw22UTDRNeuVOPiIJayoXkZD7nLGCHb4BOdgnPhn_VrOkHKlcclx-CpjRGM3TO7RfkO6cau91vm4O9xS--t8bh9TV5M4BK-OcZz8uvrl59X36vrm28_ri6vK93ULFctH4QROMhBQtsIOQKHse_M2I9a97wVjRgaKaTWndG1lgagga7mnImxazoU5-TdYe8mhr8LpqxmmzQ6Bx7DkhTvywXB-gK2B1DHkFLESW2inSFuFWdqp13ttaudUyUHtdeuhjJ3cTywjDOa09TRc-m_PfYhaXBTBK9tOmF1-S9Wy4J9PmBYZDxYjCppi16jsRF1VibY_zzkCc42oRU</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17183307</pqid></control><display><type>article</type><title>A morphometric analysis of glomerular and tubular alterations following fast-neutron irradiation of the pig and monkey kidney</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Robbins, Mike E.C ; Stephens, L.Clifton ; Johnston, Dennis A ; Thames, Howard D ; Peters, Lester J ; Hopewell, John W ; Ang, K.Kian</creator><creatorcontrib>Robbins, Mike E.C ; Stephens, L.Clifton ; Johnston, Dennis A ; Thames, Howard D ; Peters, Lester J ; Hopewell, John W ; Ang, K.Kian</creatorcontrib><description>Purpose: The morphologic responses of the pig and monkey kidney to fractionated fast-neutron irradiation were assessed.
Methods and Materials: The right kidney of approximately 14-week-old female Large White pigs was irradiated with 6.6–12.2 Gy of fast neutrons (42 MeV
d→Be) given as 12 fractions over 18 days; the left kidney served as the contralateral unirradiated kidney. Both kidneys were removed at necropsy 2 years postirradiation. In addition, the remaining hypertrophied kidney of four unilaterally nephrectomized adult rhesus monkeys was irradiated with a total dose of 11.0 Gy fast neutrons (45 MeV
p→Be) given in an identical fractionation regimen to that used in the pig studies. These kidneys were removed when the animals exhibited renal failure, between 32–94 weeks postirradiation. Glomeruli were assessed for the presence of pathologic features, including intercapillary eosinophilic material (ICE), ectatic capillaries, thrombi, hemorrhage, and sclerosis. The relative proportion of renal cortex occupied by glomeruli, interstitium, normal, or abnormal tubules was determined using a Chalkley point grid.
Results: The incidence of normal glomeruli, ectatic capillaries, thrombosis, and periglomerular fibrosis were significantly different in the irradiated pig kidneys compared with the unirradiated contralateral kidneys (
p ≤ 0.02). Linear regression analysis demonstrated a significant dose relationship in terms of normal glomeruli, ectatic capillaries, and ICE (
r ≥ 0.64;
p ≤ 0.04). Irradiation was also associated with a significant (
p < 0.0001) decrease and increase in the volume of renal cortex occupied by normal and abnormal tubules, respectively. Similar morphometric changes were noted in the irradiated monkey kidneys.
Conclusions: The morphologic changes seen in the pig and monkey kidney after fractionated irradiation with fast neutrons are similar to those previously noted after single-dose or fractionated-photon irradiation. These findings support the hypothesis that the development of radiation nephropathy in these various models involves common pathophysiological mechanisms.</description><identifier>ISSN: 0360-3016</identifier><identifier>EISSN: 1879-355X</identifier><identifier>DOI: 10.1016/S0360-3016(98)00161-8</identifier><identifier>PMID: 9719126</identifier><identifier>CODEN: IOBPD3</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Animals ; Biological and medical sciences ; Biological effects of radiation ; Blood Urea Nitrogen ; Dose Fractionation, Radiation ; Dose-Response Relationship, Radiation ; Fast Neutrons ; Fast-neutron irradiation ; Female ; Fundamental and applied biological sciences. Psychology ; Glomeruli ; Ionizing radiations ; Kidney ; Kidney - radiation effects ; Kidney Glomerulus - radiation effects ; Kidney Tubules - radiation effects ; Linear Models ; Macaca mulatta ; Monkey ; Morphometry ; Normal and abnormal tubules ; Pig ; Swine ; Tissues, organs and organisms biophysics</subject><ispartof>International journal of radiation oncology, biology, physics, 1998-07, Vol.41 (5), p.1149-1156</ispartof><rights>1998 Elsevier Science Inc.</rights><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c420t-5183d3e8989a5439ba1ab76db7bcc71534384939cc6dc2c9daa4a621103b646e3</citedby><cites>FETCH-LOGICAL-c420t-5183d3e8989a5439ba1ab76db7bcc71534384939cc6dc2c9daa4a621103b646e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0360-3016(98)00161-8$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2355029$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9719126$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Robbins, Mike E.C</creatorcontrib><creatorcontrib>Stephens, L.Clifton</creatorcontrib><creatorcontrib>Johnston, Dennis A</creatorcontrib><creatorcontrib>Thames, Howard D</creatorcontrib><creatorcontrib>Peters, Lester J</creatorcontrib><creatorcontrib>Hopewell, John W</creatorcontrib><creatorcontrib>Ang, K.Kian</creatorcontrib><title>A morphometric analysis of glomerular and tubular alterations following fast-neutron irradiation of the pig and monkey kidney</title><title>International journal of radiation oncology, biology, physics</title><addtitle>Int J Radiat Oncol Biol Phys</addtitle><description>Purpose: The morphologic responses of the pig and monkey kidney to fractionated fast-neutron irradiation were assessed.
Methods and Materials: The right kidney of approximately 14-week-old female Large White pigs was irradiated with 6.6–12.2 Gy of fast neutrons (42 MeV
d→Be) given as 12 fractions over 18 days; the left kidney served as the contralateral unirradiated kidney. Both kidneys were removed at necropsy 2 years postirradiation. In addition, the remaining hypertrophied kidney of four unilaterally nephrectomized adult rhesus monkeys was irradiated with a total dose of 11.0 Gy fast neutrons (45 MeV
p→Be) given in an identical fractionation regimen to that used in the pig studies. These kidneys were removed when the animals exhibited renal failure, between 32–94 weeks postirradiation. Glomeruli were assessed for the presence of pathologic features, including intercapillary eosinophilic material (ICE), ectatic capillaries, thrombi, hemorrhage, and sclerosis. The relative proportion of renal cortex occupied by glomeruli, interstitium, normal, or abnormal tubules was determined using a Chalkley point grid.
Results: The incidence of normal glomeruli, ectatic capillaries, thrombosis, and periglomerular fibrosis were significantly different in the irradiated pig kidneys compared with the unirradiated contralateral kidneys (
p ≤ 0.02). Linear regression analysis demonstrated a significant dose relationship in terms of normal glomeruli, ectatic capillaries, and ICE (
r ≥ 0.64;
p ≤ 0.04). Irradiation was also associated with a significant (
p < 0.0001) decrease and increase in the volume of renal cortex occupied by normal and abnormal tubules, respectively. Similar morphometric changes were noted in the irradiated monkey kidneys.
Conclusions: The morphologic changes seen in the pig and monkey kidney after fractionated irradiation with fast neutrons are similar to those previously noted after single-dose or fractionated-photon irradiation. These findings support the hypothesis that the development of radiation nephropathy in these various models involves common pathophysiological mechanisms.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biological effects of radiation</subject><subject>Blood Urea Nitrogen</subject><subject>Dose Fractionation, Radiation</subject><subject>Dose-Response Relationship, Radiation</subject><subject>Fast Neutrons</subject><subject>Fast-neutron irradiation</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glomeruli</subject><subject>Ionizing radiations</subject><subject>Kidney</subject><subject>Kidney - radiation effects</subject><subject>Kidney Glomerulus - radiation effects</subject><subject>Kidney Tubules - radiation effects</subject><subject>Linear Models</subject><subject>Macaca mulatta</subject><subject>Monkey</subject><subject>Morphometry</subject><subject>Normal and abnormal tubules</subject><subject>Pig</subject><subject>Swine</subject><subject>Tissues, organs and organisms biophysics</subject><issn>0360-3016</issn><issn>1879-355X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtvFDEMgCMEKkvhJ1TKASE4DCSTeeWEqoqXVKmHgsQt8iSebWgmWZJM0R7635t9aK-cbNmf7egLIRecfeSMd59umehYJUr6Xg4fWIm8Gp6RFR96WYm2_f2crE7IS_IqpT-sULxvzsiZ7Lnkdbcij5d0DnFzF2bM0WoKHtw22UTDRNeuVOPiIJayoXkZD7nLGCHb4BOdgnPhn_VrOkHKlcclx-CpjRGM3TO7RfkO6cau91vm4O9xS--t8bh9TV5M4BK-OcZz8uvrl59X36vrm28_ri6vK93ULFctH4QROMhBQtsIOQKHse_M2I9a97wVjRgaKaTWndG1lgagga7mnImxazoU5-TdYe8mhr8LpqxmmzQ6Bx7DkhTvywXB-gK2B1DHkFLESW2inSFuFWdqp13ttaudUyUHtdeuhjJ3cTywjDOa09TRc-m_PfYhaXBTBK9tOmF1-S9Wy4J9PmBYZDxYjCppi16jsRF1VibY_zzkCc42oRU</recordid><startdate>19980715</startdate><enddate>19980715</enddate><creator>Robbins, Mike E.C</creator><creator>Stephens, L.Clifton</creator><creator>Johnston, Dennis A</creator><creator>Thames, Howard D</creator><creator>Peters, Lester J</creator><creator>Hopewell, John W</creator><creator>Ang, K.Kian</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>19980715</creationdate><title>A morphometric analysis of glomerular and tubular alterations following fast-neutron irradiation of the pig and monkey kidney</title><author>Robbins, Mike E.C ; Stephens, L.Clifton ; Johnston, Dennis A ; Thames, Howard D ; Peters, Lester J ; Hopewell, John W ; Ang, K.Kian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c420t-5183d3e8989a5439ba1ab76db7bcc71534384939cc6dc2c9daa4a621103b646e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biological effects of radiation</topic><topic>Blood Urea Nitrogen</topic><topic>Dose Fractionation, Radiation</topic><topic>Dose-Response Relationship, Radiation</topic><topic>Fast Neutrons</topic><topic>Fast-neutron irradiation</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glomeruli</topic><topic>Ionizing radiations</topic><topic>Kidney</topic><topic>Kidney - radiation effects</topic><topic>Kidney Glomerulus - radiation effects</topic><topic>Kidney Tubules - radiation effects</topic><topic>Linear Models</topic><topic>Macaca mulatta</topic><topic>Monkey</topic><topic>Morphometry</topic><topic>Normal and abnormal tubules</topic><topic>Pig</topic><topic>Swine</topic><topic>Tissues, organs and organisms biophysics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Robbins, Mike E.C</creatorcontrib><creatorcontrib>Stephens, L.Clifton</creatorcontrib><creatorcontrib>Johnston, Dennis A</creatorcontrib><creatorcontrib>Thames, Howard D</creatorcontrib><creatorcontrib>Peters, Lester J</creatorcontrib><creatorcontrib>Hopewell, John W</creatorcontrib><creatorcontrib>Ang, K.Kian</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>International journal of radiation oncology, biology, physics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Robbins, Mike E.C</au><au>Stephens, L.Clifton</au><au>Johnston, Dennis A</au><au>Thames, Howard D</au><au>Peters, Lester J</au><au>Hopewell, John W</au><au>Ang, K.Kian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A morphometric analysis of glomerular and tubular alterations following fast-neutron irradiation of the pig and monkey kidney</atitle><jtitle>International journal of radiation oncology, biology, physics</jtitle><addtitle>Int J Radiat Oncol Biol Phys</addtitle><date>1998-07-15</date><risdate>1998</risdate><volume>41</volume><issue>5</issue><spage>1149</spage><epage>1156</epage><pages>1149-1156</pages><issn>0360-3016</issn><eissn>1879-355X</eissn><coden>IOBPD3</coden><abstract>Purpose: The morphologic responses of the pig and monkey kidney to fractionated fast-neutron irradiation were assessed.
Methods and Materials: The right kidney of approximately 14-week-old female Large White pigs was irradiated with 6.6–12.2 Gy of fast neutrons (42 MeV
d→Be) given as 12 fractions over 18 days; the left kidney served as the contralateral unirradiated kidney. Both kidneys were removed at necropsy 2 years postirradiation. In addition, the remaining hypertrophied kidney of four unilaterally nephrectomized adult rhesus monkeys was irradiated with a total dose of 11.0 Gy fast neutrons (45 MeV
p→Be) given in an identical fractionation regimen to that used in the pig studies. These kidneys were removed when the animals exhibited renal failure, between 32–94 weeks postirradiation. Glomeruli were assessed for the presence of pathologic features, including intercapillary eosinophilic material (ICE), ectatic capillaries, thrombi, hemorrhage, and sclerosis. The relative proportion of renal cortex occupied by glomeruli, interstitium, normal, or abnormal tubules was determined using a Chalkley point grid.
Results: The incidence of normal glomeruli, ectatic capillaries, thrombosis, and periglomerular fibrosis were significantly different in the irradiated pig kidneys compared with the unirradiated contralateral kidneys (
p ≤ 0.02). Linear regression analysis demonstrated a significant dose relationship in terms of normal glomeruli, ectatic capillaries, and ICE (
r ≥ 0.64;
p ≤ 0.04). Irradiation was also associated with a significant (
p < 0.0001) decrease and increase in the volume of renal cortex occupied by normal and abnormal tubules, respectively. Similar morphometric changes were noted in the irradiated monkey kidneys.
Conclusions: The morphologic changes seen in the pig and monkey kidney after fractionated irradiation with fast neutrons are similar to those previously noted after single-dose or fractionated-photon irradiation. These findings support the hypothesis that the development of radiation nephropathy in these various models involves common pathophysiological mechanisms.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>9719126</pmid><doi>10.1016/S0360-3016(98)00161-8</doi><tpages>8</tpages></addata></record> |
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| identifier | ISSN: 0360-3016 |
| ispartof | International journal of radiation oncology, biology, physics, 1998-07, Vol.41 (5), p.1149-1156 |
| issn | 0360-3016 1879-355X |
| language | eng |
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| subjects | Animals Biological and medical sciences Biological effects of radiation Blood Urea Nitrogen Dose Fractionation, Radiation Dose-Response Relationship, Radiation Fast Neutrons Fast-neutron irradiation Female Fundamental and applied biological sciences. Psychology Glomeruli Ionizing radiations Kidney Kidney - radiation effects Kidney Glomerulus - radiation effects Kidney Tubules - radiation effects Linear Models Macaca mulatta Monkey Morphometry Normal and abnormal tubules Pig Swine Tissues, organs and organisms biophysics |
| title | A morphometric analysis of glomerular and tubular alterations following fast-neutron irradiation of the pig and monkey kidney |
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