Metabolite profiling of CHO cells: Molecular reflections of bioprocessing effectiveness

Whilst development of medium and feeds has provided major advances in recombinant protein production in CHO cells, the fundamental understanding is limited. We have applied metabolite profiling with established robust (GC‐MS) analytics to define the molecular loci by which two yield‐enhancing feeds...

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Veröffentlicht in:	Biotechnology journal 2015-09, Vol.10 (9), p.1434-1445
Hauptverfasser:	Sellick, Christopher A., Croxford, Alexandra S., Maqsood, Arfa R., Stephens, Gill M., Westerhoff, Hans V., Goodacre, Royston, Dickson, Alan J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bioprocessing Biotechnology - methods CHO Cells Cricetinae Cricetulus Culture Media - metabolism Gas Chromatography-Mass Spectrometry Gas chromatography-mass spectrometry (GC-MS) Glutamine synthetase Intracellular Space - metabolism Metabolomics Metabolomics - methods Recombinant antibody Recombinant Proteins - metabolism
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