p53 target gene Rap2B regulates the cytoskeleton and inhibits cell spreading
Purpose Cell migration requires spatiotemporal integration of signals that target cytoskeletal. Previous studies have indicated that Rho GTPases are crucial regulators of actin dynamics. As homologs of Rho proteins, the role of Rap2B in the regulation of cytoskeleton and its cell signaling pathway r...
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| Veröffentlicht in: | Journal of cancer research and clinical oncology 2015-10, Vol.141 (10), p.1791-1798 |
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| container_title | Journal of cancer research and clinical oncology |
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| creator | Di, Jiehui Huang, Hui Wang, Yan Qu, Debao Tang, Juanjuan Cheng, Qian Lu, Zheng Zhang, Yanping Zheng, Junnian |
| description | Purpose
Cell migration requires spatiotemporal integration of signals that target cytoskeletal. Previous studies have indicated that Rho GTPases are crucial regulators of actin dynamics. As homologs of Rho proteins, the role of Rap2B in the regulation of cytoskeleton and its cell signaling pathway remains unknown.
Methods
The cellular functions of Rap2B were monitored by Western blotting and immunofluorescence staining in order to characterize the protein level and the cell shape.
Results
Here, we show that expression of Rap2B was induced by nocodazole in a p53-dependent manner. However, Rap2B itself is not necessary for p53-dependent cell cycle arrest. We evidenced that over-expression of Rap2B may inhibit cell spreading by disrupting actin dynamics upon nocodazole treatment, but Rap2B (C180A) mutant does not. In contrast, knockdown of Rap2B promoted cell spreading.
Conclusions
Altogether, these results revealed that Rap2B plays a pivotal role in cytoskeleton reorganization and subsequently inhibits cell spreading, which could be responsible for cancer metastasis. |
| doi_str_mv | 10.1007/s00432-015-1948-8 |
| format | Article |
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Cell migration requires spatiotemporal integration of signals that target cytoskeletal. Previous studies have indicated that Rho GTPases are crucial regulators of actin dynamics. As homologs of Rho proteins, the role of Rap2B in the regulation of cytoskeleton and its cell signaling pathway remains unknown.
Methods
The cellular functions of Rap2B were monitored by Western blotting and immunofluorescence staining in order to characterize the protein level and the cell shape.
Results
Here, we show that expression of Rap2B was induced by nocodazole in a p53-dependent manner. However, Rap2B itself is not necessary for p53-dependent cell cycle arrest. We evidenced that over-expression of Rap2B may inhibit cell spreading by disrupting actin dynamics upon nocodazole treatment, but Rap2B (C180A) mutant does not. In contrast, knockdown of Rap2B promoted cell spreading.
Conclusions
Altogether, these results revealed that Rap2B plays a pivotal role in cytoskeleton reorganization and subsequently inhibits cell spreading, which could be responsible for cancer metastasis.</description><identifier>ISSN: 0171-5216</identifier><identifier>EISSN: 1432-1335</identifier><identifier>DOI: 10.1007/s00432-015-1948-8</identifier><identifier>PMID: 25762091</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Actins - metabolism ; Cancer ; Cancer Research ; Cell Cycle Checkpoints - physiology ; Cell Line, Tumor ; Cell Movement - physiology ; Cytoskeleton ; Cytoskeleton - metabolism ; Cytoskeleton - pathology ; Genes ; Hematology ; Humans ; Internal Medicine ; Medicine ; Medicine & Public Health ; Oncology ; Original Article – Cancer Research ; Proteins ; rap GTP-Binding Proteins - metabolism ; rho GTP-Binding Proteins - metabolism ; Signal Transduction - physiology ; Studies ; Tumor Suppressor Protein p53 - metabolism</subject><ispartof>Journal of cancer research and clinical oncology, 2015-10, Vol.141 (10), p.1791-1798</ispartof><rights>Springer-Verlag Berlin Heidelberg 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c475t-47c4437b7e9bef71b2799e83b760a6bb42d06492162af11fea104552a5765af83</citedby><cites>FETCH-LOGICAL-c475t-47c4437b7e9bef71b2799e83b760a6bb42d06492162af11fea104552a5765af83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00432-015-1948-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00432-015-1948-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25762091$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Di, Jiehui</creatorcontrib><creatorcontrib>Huang, Hui</creatorcontrib><creatorcontrib>Wang, Yan</creatorcontrib><creatorcontrib>Qu, Debao</creatorcontrib><creatorcontrib>Tang, Juanjuan</creatorcontrib><creatorcontrib>Cheng, Qian</creatorcontrib><creatorcontrib>Lu, Zheng</creatorcontrib><creatorcontrib>Zhang, Yanping</creatorcontrib><creatorcontrib>Zheng, Junnian</creatorcontrib><title>p53 target gene Rap2B regulates the cytoskeleton and inhibits cell spreading</title><title>Journal of cancer research and clinical oncology</title><addtitle>J Cancer Res Clin Oncol</addtitle><addtitle>J Cancer Res Clin Oncol</addtitle><description>Purpose
Cell migration requires spatiotemporal integration of signals that target cytoskeletal. Previous studies have indicated that Rho GTPases are crucial regulators of actin dynamics. As homologs of Rho proteins, the role of Rap2B in the regulation of cytoskeleton and its cell signaling pathway remains unknown.
Methods
The cellular functions of Rap2B were monitored by Western blotting and immunofluorescence staining in order to characterize the protein level and the cell shape.
Results
Here, we show that expression of Rap2B was induced by nocodazole in a p53-dependent manner. However, Rap2B itself is not necessary for p53-dependent cell cycle arrest. We evidenced that over-expression of Rap2B may inhibit cell spreading by disrupting actin dynamics upon nocodazole treatment, but Rap2B (C180A) mutant does not. In contrast, knockdown of Rap2B promoted cell spreading.
Conclusions
Altogether, these results revealed that Rap2B plays a pivotal role in cytoskeleton reorganization and subsequently inhibits cell spreading, which could be responsible for cancer metastasis.</description><subject>Actins - metabolism</subject><subject>Cancer</subject><subject>Cancer Research</subject><subject>Cell Cycle Checkpoints - physiology</subject><subject>Cell Line, Tumor</subject><subject>Cell Movement - physiology</subject><subject>Cytoskeleton</subject><subject>Cytoskeleton - metabolism</subject><subject>Cytoskeleton - pathology</subject><subject>Genes</subject><subject>Hematology</subject><subject>Humans</subject><subject>Internal Medicine</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Oncology</subject><subject>Original Article – Cancer Research</subject><subject>Proteins</subject><subject>rap GTP-Binding Proteins - metabolism</subject><subject>rho GTP-Binding Proteins - metabolism</subject><subject>Signal Transduction - physiology</subject><subject>Studies</subject><subject>Tumor Suppressor Protein p53 - metabolism</subject><issn>0171-5216</issn><issn>1432-1335</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqNkU9LxDAQxYMouq5-AC8S8OKlmkmTpj3q4j9YEETPIe1Ou127bU3Sw357U3YVEQRPYcJv3ryZR8gZsCtgTF07xkTMIwYygkykUbpHJjD-QBzLfTJhoCCSHJIjcuzcioVaKn5IjrhUCWcZTMi8lzH1xlboaYUt0hfT81tqsRoa49FRv0RabHzn3rFB37XUtAtat8s6r72jBTYNdb1Fs6jb6oQclKZxeLp7p-Tt_u519hjNnx-eZjfzqBBK-kioQohY5QqzHEsFOVdZhmmcq4SZJM8FX7BEZME3NyVAiQaYkJKb4FqaMo2n5HKr29vuY0Dn9bp2oxXTYjc4HdZWIgOI4R8oC5eQGVMBvfiFrrrBtmGRkZKJEIyPs2FLFbZzzmKpe1uvjd1oYHpMRW9T0SEVPaaix57znfKQr3Hx3fEVQwD4FginDHdE-2P0n6qf8FaUlw</recordid><startdate>20151001</startdate><enddate>20151001</enddate><creator>Di, Jiehui</creator><creator>Huang, Hui</creator><creator>Wang, Yan</creator><creator>Qu, Debao</creator><creator>Tang, Juanjuan</creator><creator>Cheng, Qian</creator><creator>Lu, Zheng</creator><creator>Zhang, Yanping</creator><creator>Zheng, Junnian</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20151001</creationdate><title>p53 target gene Rap2B regulates the cytoskeleton and inhibits cell spreading</title><author>Di, Jiehui ; Huang, Hui ; Wang, Yan ; Qu, Debao ; Tang, Juanjuan ; Cheng, Qian ; Lu, Zheng ; Zhang, Yanping ; Zheng, Junnian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c475t-47c4437b7e9bef71b2799e83b760a6bb42d06492162af11fea104552a5765af83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Actins - metabolism</topic><topic>Cancer</topic><topic>Cancer Research</topic><topic>Cell Cycle Checkpoints - physiology</topic><topic>Cell Line, Tumor</topic><topic>Cell Movement - physiology</topic><topic>Cytoskeleton</topic><topic>Cytoskeleton - metabolism</topic><topic>Cytoskeleton - pathology</topic><topic>Genes</topic><topic>Hematology</topic><topic>Humans</topic><topic>Internal Medicine</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Oncology</topic><topic>Original Article – Cancer Research</topic><topic>Proteins</topic><topic>rap GTP-Binding Proteins - metabolism</topic><topic>rho GTP-Binding Proteins - metabolism</topic><topic>Signal Transduction - physiology</topic><topic>Studies</topic><topic>Tumor Suppressor Protein p53 - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Di, Jiehui</creatorcontrib><creatorcontrib>Huang, Hui</creatorcontrib><creatorcontrib>Wang, Yan</creatorcontrib><creatorcontrib>Qu, Debao</creatorcontrib><creatorcontrib>Tang, Juanjuan</creatorcontrib><creatorcontrib>Cheng, Qian</creatorcontrib><creatorcontrib>Lu, Zheng</creatorcontrib><creatorcontrib>Zhang, Yanping</creatorcontrib><creatorcontrib>Zheng, Junnian</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of cancer research and clinical oncology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Di, Jiehui</au><au>Huang, Hui</au><au>Wang, Yan</au><au>Qu, Debao</au><au>Tang, Juanjuan</au><au>Cheng, Qian</au><au>Lu, Zheng</au><au>Zhang, Yanping</au><au>Zheng, Junnian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>p53 target gene Rap2B regulates the cytoskeleton and inhibits cell spreading</atitle><jtitle>Journal of cancer research and clinical oncology</jtitle><stitle>J Cancer Res Clin Oncol</stitle><addtitle>J Cancer Res Clin Oncol</addtitle><date>2015-10-01</date><risdate>2015</risdate><volume>141</volume><issue>10</issue><spage>1791</spage><epage>1798</epage><pages>1791-1798</pages><issn>0171-5216</issn><eissn>1432-1335</eissn><abstract>Purpose
Cell migration requires spatiotemporal integration of signals that target cytoskeletal. Previous studies have indicated that Rho GTPases are crucial regulators of actin dynamics. As homologs of Rho proteins, the role of Rap2B in the regulation of cytoskeleton and its cell signaling pathway remains unknown.
Methods
The cellular functions of Rap2B were monitored by Western blotting and immunofluorescence staining in order to characterize the protein level and the cell shape.
Results
Here, we show that expression of Rap2B was induced by nocodazole in a p53-dependent manner. However, Rap2B itself is not necessary for p53-dependent cell cycle arrest. We evidenced that over-expression of Rap2B may inhibit cell spreading by disrupting actin dynamics upon nocodazole treatment, but Rap2B (C180A) mutant does not. In contrast, knockdown of Rap2B promoted cell spreading.
Conclusions
Altogether, these results revealed that Rap2B plays a pivotal role in cytoskeleton reorganization and subsequently inhibits cell spreading, which could be responsible for cancer metastasis.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>25762091</pmid><doi>10.1007/s00432-015-1948-8</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0171-5216 |
| ispartof | Journal of cancer research and clinical oncology, 2015-10, Vol.141 (10), p.1791-1798 |
| issn | 0171-5216 1432-1335 |
| language | eng |
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| source | MEDLINE; SpringerNature Journals |
| subjects | Actins - metabolism Cancer Cancer Research Cell Cycle Checkpoints - physiology Cell Line, Tumor Cell Movement - physiology Cytoskeleton Cytoskeleton - metabolism Cytoskeleton - pathology Genes Hematology Humans Internal Medicine Medicine Medicine & Public Health Oncology Original Article – Cancer Research Proteins rap GTP-Binding Proteins - metabolism rho GTP-Binding Proteins - metabolism Signal Transduction - physiology Studies Tumor Suppressor Protein p53 - metabolism |
| title | p53 target gene Rap2B regulates the cytoskeleton and inhibits cell spreading |
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