Analytical Ultracentrifugation and Its Role in Development and Research of Therapeutical Proteins
The historical contributions of analytical ultracentrifugation (AUC) to modern biology and biotechnology drug development and research are discussed. AUC developed by Svedberg was used to show that proteins are actually large defined molecular entities and also provided the first experimental verifi...
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| Veröffentlicht in: | Methods in enzymology 2015, Vol.562, p.441-476 |
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| container_title | Methods in enzymology |
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| creator | Liu, Jun Yadav, Sandeep Andya, James Demeule, Barthélemy Shire, Steven J |
| description | The historical contributions of analytical ultracentrifugation (AUC) to modern biology and biotechnology drug development and research are discussed. AUC developed by Svedberg was used to show that proteins are actually large defined molecular entities and also provided the first experimental verification for the semiconservative replication model for DNA initially proposed by Watson and Crick. This chapter reviews the use of AUC to investigate molecular weight of recombinant-DNA-produced proteins, complex formation of antibodies, intermolecular interactions in dilute and high concentration protein solution, and their impact on physical properties such as solution viscosity. Recent studies using a "competitive binding" analysis by AUC have been useful in critically evaluating the design and interpretation of surface plasmon resonance measurements and are discussed. The future of this technology is also discussed including prospects for a new higher precision analytical ultracentrifuge. |
| doi_str_mv | 10.1016/bs.mie.2015.04.008 |
| format | Article |
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AUC developed by Svedberg was used to show that proteins are actually large defined molecular entities and also provided the first experimental verification for the semiconservative replication model for DNA initially proposed by Watson and Crick. This chapter reviews the use of AUC to investigate molecular weight of recombinant-DNA-produced proteins, complex formation of antibodies, intermolecular interactions in dilute and high concentration protein solution, and their impact on physical properties such as solution viscosity. Recent studies using a "competitive binding" analysis by AUC have been useful in critically evaluating the design and interpretation of surface plasmon resonance measurements and are discussed. The future of this technology is also discussed including prospects for a new higher precision analytical ultracentrifuge.</description><identifier>EISSN: 1557-7988</identifier><identifier>DOI: 10.1016/bs.mie.2015.04.008</identifier><identifier>PMID: 26412663</identifier><language>eng</language><publisher>United States</publisher><subject>Algorithms ; Antibodies, Monoclonal - chemistry ; Antibodies, Monoclonal - isolation & purification ; Binding, Competitive ; Drug Discovery ; Humans ; Models, Molecular ; Molecular Weight ; Protein Binding ; Recombinant Proteins - chemistry ; Recombinant Proteins - isolation & purification ; Ultracentrifugation</subject><ispartof>Methods in enzymology, 2015, Vol.562, p.441-476</ispartof><rights>2015 Elsevier Inc. 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AUC developed by Svedberg was used to show that proteins are actually large defined molecular entities and also provided the first experimental verification for the semiconservative replication model for DNA initially proposed by Watson and Crick. This chapter reviews the use of AUC to investigate molecular weight of recombinant-DNA-produced proteins, complex formation of antibodies, intermolecular interactions in dilute and high concentration protein solution, and their impact on physical properties such as solution viscosity. Recent studies using a "competitive binding" analysis by AUC have been useful in critically evaluating the design and interpretation of surface plasmon resonance measurements and are discussed. The future of this technology is also discussed including prospects for a new higher precision analytical ultracentrifuge.</description><subject>Algorithms</subject><subject>Antibodies, Monoclonal - chemistry</subject><subject>Antibodies, Monoclonal - isolation & purification</subject><subject>Binding, Competitive</subject><subject>Drug Discovery</subject><subject>Humans</subject><subject>Models, Molecular</subject><subject>Molecular Weight</subject><subject>Protein Binding</subject><subject>Recombinant Proteins - chemistry</subject><subject>Recombinant Proteins - isolation & purification</subject><subject>Ultracentrifugation</subject><issn>1557-7988</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kMtOwzAURC0kREvhB1ggL9k02HH8yLIqr0qVQFW7jm6Sa2rkPIgdpP49BYpmMYs5Oosh5IazhDOu7suQNA6TlHGZsCxhzJyRKZdSz3VuzIRchvDBWKpNzi_IJFUZT5USUwKLFvwhugo83fk4QIVtHJwd3yG6rqXQ1nQVA910Hqlr6QN-oe_65kj9bhsMCEO1p52l2z0O0OP4Z3sbuoiuDVfk3IIPeH3qGdk9PW6XL_P16_NquVjPK5GrOJdlKuqs1iilSY0GDZYLwVXNS8m54jLPSkAtc5VKwFILUMpWxlpmstJmuZiRuz9vP3SfI4ZYNC5U6D202I2h4JrrzPzkiN6e0LFssC76wTUwHIr_W8Q3sYNkYw</recordid><startdate>2015</startdate><enddate>2015</enddate><creator>Liu, Jun</creator><creator>Yadav, Sandeep</creator><creator>Andya, James</creator><creator>Demeule, Barthélemy</creator><creator>Shire, Steven J</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>2015</creationdate><title>Analytical Ultracentrifugation and Its Role in Development and Research of Therapeutical Proteins</title><author>Liu, Jun ; Yadav, Sandeep ; Andya, James ; Demeule, Barthélemy ; Shire, Steven J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c396t-5b23d4d7e558287a7af13316d1b51161594bae759625aeb73a66fc8ff084bf493</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Algorithms</topic><topic>Antibodies, Monoclonal - chemistry</topic><topic>Antibodies, Monoclonal - isolation & purification</topic><topic>Binding, Competitive</topic><topic>Drug Discovery</topic><topic>Humans</topic><topic>Models, Molecular</topic><topic>Molecular Weight</topic><topic>Protein Binding</topic><topic>Recombinant Proteins - chemistry</topic><topic>Recombinant Proteins - isolation & purification</topic><topic>Ultracentrifugation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Jun</creatorcontrib><creatorcontrib>Yadav, Sandeep</creatorcontrib><creatorcontrib>Andya, James</creatorcontrib><creatorcontrib>Demeule, Barthélemy</creatorcontrib><creatorcontrib>Shire, Steven J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Methods in enzymology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Jun</au><au>Yadav, Sandeep</au><au>Andya, James</au><au>Demeule, Barthélemy</au><au>Shire, Steven J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analytical Ultracentrifugation and Its Role in Development and Research of Therapeutical Proteins</atitle><jtitle>Methods in enzymology</jtitle><addtitle>Methods Enzymol</addtitle><date>2015</date><risdate>2015</risdate><volume>562</volume><spage>441</spage><epage>476</epage><pages>441-476</pages><eissn>1557-7988</eissn><abstract>The historical contributions of analytical ultracentrifugation (AUC) to modern biology and biotechnology drug development and research are discussed. 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| identifier | EISSN: 1557-7988 |
| ispartof | Methods in enzymology, 2015, Vol.562, p.441-476 |
| issn | 1557-7988 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Algorithms Antibodies, Monoclonal - chemistry Antibodies, Monoclonal - isolation & purification Binding, Competitive Drug Discovery Humans Models, Molecular Molecular Weight Protein Binding Recombinant Proteins - chemistry Recombinant Proteins - isolation & purification Ultracentrifugation |
| title | Analytical Ultracentrifugation and Its Role in Development and Research of Therapeutical Proteins |
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