Characterization of DNA damage induced by gamma-radiation-derived water radicals, using DNA repair enzymes
To characterize the DNA damage profiles due to gamma-radiation induced water radicals. Double stranded (ds) phiX174 DNA was irradiated in aqueous solution with gamma-rays under different gassing conditions (O2, N2O or N2) and the damage profiles were determined using DNA repair enzymes. The DNA dama...
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| Veröffentlicht in: | International journal of radiation biology 1998-10, Vol.74 (4), p.511-519 |
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| creator | KUIPERS, G. K LAFLEUR, M. M |
| description | To characterize the DNA damage profiles due to gamma-radiation induced water radicals.
Double stranded (ds) phiX174 DNA was irradiated in aqueous solution with gamma-rays under different gassing conditions (O2, N2O or N2) and the damage profiles were determined using DNA repair enzymes.
The DNA damage profile under O2 is characterized by about equal numbers of direct single-strand breaks (ssb) and Fpg sensitive sites, whereas endonuclease III and exonuclease III sites are formed in lower amounts. The DNA damage profiles under N2O and N2 in phosphate buffer consist predominantly of direct single-strand breaks. Fpg sensitive sites dominate the DNA damage profile under N2 in phosphate buffer in the presence of the radical scavenger 2-methyl propan-2-ol, where (almost) only .H atoms are present.
Both .OH radicals and .H atoms induce direct single-strand breaks, but .OH radicals are the most effective ones. Fpg sensitive sites are induced in high amounts by both .OH radicals and H atoms, but when both types of radicals are present, the formation of Fpg sensitive sites is prevented. Hydrated electrons (e(aq)-) contribute to inactivation of DNA, although only a very small fraction of the e(aq)- is involved in this process. |
| doi_str_mv | 10.1080/095530098141384 |
| format | Article |
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Double stranded (ds) phiX174 DNA was irradiated in aqueous solution with gamma-rays under different gassing conditions (O2, N2O or N2) and the damage profiles were determined using DNA repair enzymes.
The DNA damage profile under O2 is characterized by about equal numbers of direct single-strand breaks (ssb) and Fpg sensitive sites, whereas endonuclease III and exonuclease III sites are formed in lower amounts. The DNA damage profiles under N2O and N2 in phosphate buffer consist predominantly of direct single-strand breaks. Fpg sensitive sites dominate the DNA damage profile under N2 in phosphate buffer in the presence of the radical scavenger 2-methyl propan-2-ol, where (almost) only .H atoms are present.
Both .OH radicals and .H atoms induce direct single-strand breaks, but .OH radicals are the most effective ones. Fpg sensitive sites are induced in high amounts by both .OH radicals and H atoms, but when both types of radicals are present, the formation of Fpg sensitive sites is prevented. Hydrated electrons (e(aq)-) contribute to inactivation of DNA, although only a very small fraction of the e(aq)- is involved in this process.</description><identifier>ISSN: 0955-3002</identifier><identifier>EISSN: 1362-3095</identifier><identifier>DOI: 10.1080/095530098141384</identifier><identifier>PMID: 9798962</identifier><language>eng</language><publisher>London: Taylor & Francis</publisher><subject>Bacteriophage phi X 174 - genetics ; Biological and medical sciences ; Deoxyribonuclease (Pyrimidine Dimer) ; DNA - radiation effects ; DNA Damage - genetics ; DNA Repair - genetics ; DNA, Viral - radiation effects ; Dose-Response Relationship, Radiation ; Endodeoxyribonucleases - metabolism ; Escherichia coli Proteins ; Exodeoxyribonucleases - metabolism ; Free Radical Scavengers - metabolism ; Free Radicals - pharmacology ; Fundamental and applied biological sciences. Psychology ; Gamma Rays - adverse effects ; Molecular and cellular biology ; Molecular genetics ; Mutagenesis. Repair ; Nitrogen - metabolism ; Nitrous Oxide - metabolism ; Oxygen - metabolism ; tert-Butyl Alcohol - metabolism ; Water - metabolism</subject><ispartof>International journal of radiation biology, 1998-10, Vol.74 (4), p.511-519</ispartof><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2426081$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9798962$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>KUIPERS, G. K</creatorcontrib><creatorcontrib>LAFLEUR, M. M</creatorcontrib><title>Characterization of DNA damage induced by gamma-radiation-derived water radicals, using DNA repair enzymes</title><title>International journal of radiation biology</title><addtitle>Int J Radiat Biol</addtitle><description>To characterize the DNA damage profiles due to gamma-radiation induced water radicals.
Double stranded (ds) phiX174 DNA was irradiated in aqueous solution with gamma-rays under different gassing conditions (O2, N2O or N2) and the damage profiles were determined using DNA repair enzymes.
The DNA damage profile under O2 is characterized by about equal numbers of direct single-strand breaks (ssb) and Fpg sensitive sites, whereas endonuclease III and exonuclease III sites are formed in lower amounts. The DNA damage profiles under N2O and N2 in phosphate buffer consist predominantly of direct single-strand breaks. Fpg sensitive sites dominate the DNA damage profile under N2 in phosphate buffer in the presence of the radical scavenger 2-methyl propan-2-ol, where (almost) only .H atoms are present.
Both .OH radicals and .H atoms induce direct single-strand breaks, but .OH radicals are the most effective ones. Fpg sensitive sites are induced in high amounts by both .OH radicals and H atoms, but when both types of radicals are present, the formation of Fpg sensitive sites is prevented. Hydrated electrons (e(aq)-) contribute to inactivation of DNA, although only a very small fraction of the e(aq)- is involved in this process.</description><subject>Bacteriophage phi X 174 - genetics</subject><subject>Biological and medical sciences</subject><subject>Deoxyribonuclease (Pyrimidine Dimer)</subject><subject>DNA - radiation effects</subject><subject>DNA Damage - genetics</subject><subject>DNA Repair - genetics</subject><subject>DNA, Viral - radiation effects</subject><subject>Dose-Response Relationship, Radiation</subject><subject>Endodeoxyribonucleases - metabolism</subject><subject>Escherichia coli Proteins</subject><subject>Exodeoxyribonucleases - metabolism</subject><subject>Free Radical Scavengers - metabolism</subject><subject>Free Radicals - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gamma Rays - adverse effects</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Mutagenesis. Repair</subject><subject>Nitrogen - metabolism</subject><subject>Nitrous Oxide - metabolism</subject><subject>Oxygen - metabolism</subject><subject>tert-Butyl Alcohol - metabolism</subject><subject>Water - metabolism</subject><issn>0955-3002</issn><issn>1362-3095</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kDtPAzEQhC0ECiFQUyG5QFQc-HU-u4zCU4qggfq0flxwlLsEOwdKfj0ORFQ72vlmpF2Ezim5oUSRW6LLkhOiFRWUK3GAhpRLVvC8P0TDnZs1YcfoJKU5yYpwNUADXWmlJRui-eQDIti1j2EL67Ds8LLBdy9j7KCFmcehc731DpsNnkHbQhHBhV-wcDnzla1vyGm821tYpGvcp9DNfjuiX0GI2HfbTevTKTpqMuDP9nOE3h_u3yZPxfT18XkynhYrJuW6YERXsvENWM5NKaylGqQUVWUq64yzoIw3pRTCV5SaiiqRtTFMCMeMloSP0NVf7youP3uf1nUbkvWLBXR-2aeaVlRqQVUGL_Zgb1rv6lUMLcRNvX9O9i_3PqR8WhOhsyH9Y0wwSRTlPwArc2E</recordid><startdate>19981001</startdate><enddate>19981001</enddate><creator>KUIPERS, G. K</creator><creator>LAFLEUR, M. M</creator><general>Taylor & Francis</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>19981001</creationdate><title>Characterization of DNA damage induced by gamma-radiation-derived water radicals, using DNA repair enzymes</title><author>KUIPERS, G. K ; LAFLEUR, M. M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p266t-20976fefac33b54cc19a66477b7cdbdca8beb5644e711b7184644bb244d2b9603</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Bacteriophage phi X 174 - genetics</topic><topic>Biological and medical sciences</topic><topic>Deoxyribonuclease (Pyrimidine Dimer)</topic><topic>DNA - radiation effects</topic><topic>DNA Damage - genetics</topic><topic>DNA Repair - genetics</topic><topic>DNA, Viral - radiation effects</topic><topic>Dose-Response Relationship, Radiation</topic><topic>Endodeoxyribonucleases - metabolism</topic><topic>Escherichia coli Proteins</topic><topic>Exodeoxyribonucleases - metabolism</topic><topic>Free Radical Scavengers - metabolism</topic><topic>Free Radicals - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gamma Rays - adverse effects</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Mutagenesis. Repair</topic><topic>Nitrogen - metabolism</topic><topic>Nitrous Oxide - metabolism</topic><topic>Oxygen - metabolism</topic><topic>tert-Butyl Alcohol - metabolism</topic><topic>Water - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>KUIPERS, G. K</creatorcontrib><creatorcontrib>LAFLEUR, M. M</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>International journal of radiation biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>KUIPERS, G. K</au><au>LAFLEUR, M. M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of DNA damage induced by gamma-radiation-derived water radicals, using DNA repair enzymes</atitle><jtitle>International journal of radiation biology</jtitle><addtitle>Int J Radiat Biol</addtitle><date>1998-10-01</date><risdate>1998</risdate><volume>74</volume><issue>4</issue><spage>511</spage><epage>519</epage><pages>511-519</pages><issn>0955-3002</issn><eissn>1362-3095</eissn><abstract>To characterize the DNA damage profiles due to gamma-radiation induced water radicals.
Double stranded (ds) phiX174 DNA was irradiated in aqueous solution with gamma-rays under different gassing conditions (O2, N2O or N2) and the damage profiles were determined using DNA repair enzymes.
The DNA damage profile under O2 is characterized by about equal numbers of direct single-strand breaks (ssb) and Fpg sensitive sites, whereas endonuclease III and exonuclease III sites are formed in lower amounts. The DNA damage profiles under N2O and N2 in phosphate buffer consist predominantly of direct single-strand breaks. Fpg sensitive sites dominate the DNA damage profile under N2 in phosphate buffer in the presence of the radical scavenger 2-methyl propan-2-ol, where (almost) only .H atoms are present.
Both .OH radicals and .H atoms induce direct single-strand breaks, but .OH radicals are the most effective ones. Fpg sensitive sites are induced in high amounts by both .OH radicals and H atoms, but when both types of radicals are present, the formation of Fpg sensitive sites is prevented. Hydrated electrons (e(aq)-) contribute to inactivation of DNA, although only a very small fraction of the e(aq)- is involved in this process.</abstract><cop>London</cop><pub>Taylor & Francis</pub><pmid>9798962</pmid><doi>10.1080/095530098141384</doi><tpages>9</tpages></addata></record> |
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| ispartof | International journal of radiation biology, 1998-10, Vol.74 (4), p.511-519 |
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| source | MEDLINE; Taylor & Francis Medical Library - CRKN; Taylor & Francis Journals Complete |
| subjects | Bacteriophage phi X 174 - genetics Biological and medical sciences Deoxyribonuclease (Pyrimidine Dimer) DNA - radiation effects DNA Damage - genetics DNA Repair - genetics DNA, Viral - radiation effects Dose-Response Relationship, Radiation Endodeoxyribonucleases - metabolism Escherichia coli Proteins Exodeoxyribonucleases - metabolism Free Radical Scavengers - metabolism Free Radicals - pharmacology Fundamental and applied biological sciences. Psychology Gamma Rays - adverse effects Molecular and cellular biology Molecular genetics Mutagenesis. Repair Nitrogen - metabolism Nitrous Oxide - metabolism Oxygen - metabolism tert-Butyl Alcohol - metabolism Water - metabolism |
| title | Characterization of DNA damage induced by gamma-radiation-derived water radicals, using DNA repair enzymes |
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