Role of Th1 and Th17 cells in the development and complexity of coronary artery disease: comparison analysis by the methods of flow cytometry and SYNTAX score
BACKGROUNDThere have been few reports on the relationship between the expression of the CD4 T cells producing interferon-γ (Th1)/interleukin-17 (Th17) and degree of atherosclerosis. Thus, we analyzed Th1 and Th17 cell frequencies in patients with noncardiac chest pain (control), stable angina (SA),...
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| Veröffentlicht in: | Coronary artery disease 2015-11, Vol.26 (7), p.604-611 |
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| creator | Kim, Ju-Dong Lee, Seung-Hyun Seo, Eun-Hye Woo, So-Yon Kim, Sung-Hea Chung, Sang-Man Kim, Hyun-Joong |
| description | BACKGROUNDThere have been few reports on the relationship between the expression of the CD4 T cells producing interferon-γ (Th1)/interleukin-17 (Th17) and degree of atherosclerosis. Thus, we analyzed Th1 and Th17 cell frequencies in patients with noncardiac chest pain (control), stable angina (SA), and acute myocardial infarction (AMI), and compared the complexity of the coronary artery with the SYNTAX score.
PATIENTS AND METHODSThis study included 124 patients with a complaint of chest pain who underwent coronary angiography (control30 patients, SA47 patients, AMI47 patients). Peripheral blood was sampled during coronary angiography. Mononuclear cells from patients were stimulated for 4 h ex vivo. After staining with specific antibodies and fluorescence, the frequencies of Th1 and Th17 cells were measured by flow cytometry. The SYNTAX score was calculated by coronary angiography and a web-based calculator.
RESULTSThere was no significant difference in the baseline characteristics, except the higher frequencies of hypertension in SA patients (76.1%) and smoking in AMI patients (53.3%). Patients with SA showed a significantly higher frequency of Th1 cells (21.56±9.57%) compared with controls (14.84±8.58%) and patients with AMI (9.04±7.02%) (P |
| doi_str_mv | 10.1097/MCA.0000000000000289 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1716934405</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1716934405</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3059-8fb11fd2842c697379dce737bd46bfb0854df33caebdf6fcea63a16eb6c4733d3</originalsourceid><addsrcrecordid>eNp9kcFu1DAYhC1ERZfCGyDkI5cUO3acuLfVqgWkUiRYJDhFjv1bCXXirZ3tkpfhWevslqrqob6MZM18vzSD0DtKTimR5cevq-UpefzySr5AC8pLlhUVIy_RgsiCZ0Lm1TF6HeMfQigvyuIVOs4FrYiUxQL9--4dYG_xuqVYDWbWEmtwLuJuwGML2MAtOL_pYRj3Du37jYO_3TjNOe2DH1SYsAojJDFdBBXhbG9ToYt-SCnlpthF3Ex7Yg9j602c49b5HdbT6NPfDEn8H7-v1stfOCYyvEFHVrkIb-_1BP28OF-vPmeX3z59WS0vM81IIbPKNpRak1c810KWrJRGQ5LGcNHYhlQFN5YxraAxVlgNSjBFBTRCp7qYYSfow4G7Cf5mC3Gs-y7OLagB_DbWtKRCMs5Jkaz8YNXBxxjA1pvQ9amBmpJ6XqZOy9RPl0mx9_cXtk0P5iH0f4pkqA6GnXepyXjttjsIdQvKje3z7DsPqZzA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1716934405</pqid></control><display><type>article</type><title>Role of Th1 and Th17 cells in the development and complexity of coronary artery disease: comparison analysis by the methods of flow cytometry and SYNTAX score</title><source>MEDLINE</source><source>Journals@Ovid Complete</source><creator>Kim, Ju-Dong ; Lee, Seung-Hyun ; Seo, Eun-Hye ; Woo, So-Yon ; Kim, Sung-Hea ; Chung, Sang-Man ; Kim, Hyun-Joong</creator><creatorcontrib>Kim, Ju-Dong ; Lee, Seung-Hyun ; Seo, Eun-Hye ; Woo, So-Yon ; Kim, Sung-Hea ; Chung, Sang-Man ; Kim, Hyun-Joong</creatorcontrib><description>BACKGROUNDThere have been few reports on the relationship between the expression of the CD4 T cells producing interferon-γ (Th1)/interleukin-17 (Th17) and degree of atherosclerosis. Thus, we analyzed Th1 and Th17 cell frequencies in patients with noncardiac chest pain (control), stable angina (SA), and acute myocardial infarction (AMI), and compared the complexity of the coronary artery with the SYNTAX score.
PATIENTS AND METHODSThis study included 124 patients with a complaint of chest pain who underwent coronary angiography (control30 patients, SA47 patients, AMI47 patients). Peripheral blood was sampled during coronary angiography. Mononuclear cells from patients were stimulated for 4 h ex vivo. After staining with specific antibodies and fluorescence, the frequencies of Th1 and Th17 cells were measured by flow cytometry. The SYNTAX score was calculated by coronary angiography and a web-based calculator.
RESULTSThere was no significant difference in the baseline characteristics, except the higher frequencies of hypertension in SA patients (76.1%) and smoking in AMI patients (53.3%). Patients with SA showed a significantly higher frequency of Th1 cells (21.56±9.57%) compared with controls (14.84±8.58%) and patients with AMI (9.04±7.02%) (P<0.001). The frequency of Th17 cells also increased in SA patients (control1.90±1.05%, SA2.96±1.42%, AMI1.32±0.92%, P<0.001). The SYNTAX score was significantly higher in SA patients (SA21.51±11.67, AMI15.36±8.84, P=0.006) and correlated with the frequencies of Th1 and Th17 cells (r=0.359, P=0.001; r=0.248, P=0.031; respectively).
CONCLUSIONTh1 and Th17 cells were related to the development of SA, but not AMI. They could be a useful marker for the complexity of atherosclerosis in coronary artery disease.</description><identifier>ISSN: 0954-6928</identifier><identifier>EISSN: 1473-5830</identifier><identifier>DOI: 10.1097/MCA.0000000000000289</identifier><identifier>PMID: 26180995</identifier><language>eng</language><publisher>England: Copyright Wolters Kluwer Health, Inc. All rights reserved</publisher><subject>Aged ; Angina, Stable - diagnostic imaging ; Angina, Stable - immunology ; Coronary Angiography ; Coronary Artery Disease - diagnostic imaging ; Coronary Artery Disease - immunology ; Female ; Flow Cytometry ; Humans ; Interferon-gamma - immunology ; Interleukin-17 - immunology ; Male ; Middle Aged ; Myocardial Infarction - diagnostic imaging ; Myocardial Infarction - immunology ; Severity of Illness Index ; Th1 Cells - immunology ; Th17 Cells - immunology</subject><ispartof>Coronary artery disease, 2015-11, Vol.26 (7), p.604-611</ispartof><rights>Copyright © 2015 Wolters Kluwer Health, Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c3059-8fb11fd2842c697379dce737bd46bfb0854df33caebdf6fcea63a16eb6c4733d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26180995$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Ju-Dong</creatorcontrib><creatorcontrib>Lee, Seung-Hyun</creatorcontrib><creatorcontrib>Seo, Eun-Hye</creatorcontrib><creatorcontrib>Woo, So-Yon</creatorcontrib><creatorcontrib>Kim, Sung-Hea</creatorcontrib><creatorcontrib>Chung, Sang-Man</creatorcontrib><creatorcontrib>Kim, Hyun-Joong</creatorcontrib><title>Role of Th1 and Th17 cells in the development and complexity of coronary artery disease: comparison analysis by the methods of flow cytometry and SYNTAX score</title><title>Coronary artery disease</title><addtitle>Coron Artery Dis</addtitle><description>BACKGROUNDThere have been few reports on the relationship between the expression of the CD4 T cells producing interferon-γ (Th1)/interleukin-17 (Th17) and degree of atherosclerosis. Thus, we analyzed Th1 and Th17 cell frequencies in patients with noncardiac chest pain (control), stable angina (SA), and acute myocardial infarction (AMI), and compared the complexity of the coronary artery with the SYNTAX score.
PATIENTS AND METHODSThis study included 124 patients with a complaint of chest pain who underwent coronary angiography (control30 patients, SA47 patients, AMI47 patients). Peripheral blood was sampled during coronary angiography. Mononuclear cells from patients were stimulated for 4 h ex vivo. After staining with specific antibodies and fluorescence, the frequencies of Th1 and Th17 cells were measured by flow cytometry. The SYNTAX score was calculated by coronary angiography and a web-based calculator.
RESULTSThere was no significant difference in the baseline characteristics, except the higher frequencies of hypertension in SA patients (76.1%) and smoking in AMI patients (53.3%). Patients with SA showed a significantly higher frequency of Th1 cells (21.56±9.57%) compared with controls (14.84±8.58%) and patients with AMI (9.04±7.02%) (P<0.001). The frequency of Th17 cells also increased in SA patients (control1.90±1.05%, SA2.96±1.42%, AMI1.32±0.92%, P<0.001). The SYNTAX score was significantly higher in SA patients (SA21.51±11.67, AMI15.36±8.84, P=0.006) and correlated with the frequencies of Th1 and Th17 cells (r=0.359, P=0.001; r=0.248, P=0.031; respectively).
CONCLUSIONTh1 and Th17 cells were related to the development of SA, but not AMI. They could be a useful marker for the complexity of atherosclerosis in coronary artery disease.</description><subject>Aged</subject><subject>Angina, Stable - diagnostic imaging</subject><subject>Angina, Stable - immunology</subject><subject>Coronary Angiography</subject><subject>Coronary Artery Disease - diagnostic imaging</subject><subject>Coronary Artery Disease - immunology</subject><subject>Female</subject><subject>Flow Cytometry</subject><subject>Humans</subject><subject>Interferon-gamma - immunology</subject><subject>Interleukin-17 - immunology</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Myocardial Infarction - diagnostic imaging</subject><subject>Myocardial Infarction - immunology</subject><subject>Severity of Illness Index</subject><subject>Th1 Cells - immunology</subject><subject>Th17 Cells - immunology</subject><issn>0954-6928</issn><issn>1473-5830</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcFu1DAYhC1ERZfCGyDkI5cUO3acuLfVqgWkUiRYJDhFjv1bCXXirZ3tkpfhWevslqrqob6MZM18vzSD0DtKTimR5cevq-UpefzySr5AC8pLlhUVIy_RgsiCZ0Lm1TF6HeMfQigvyuIVOs4FrYiUxQL9--4dYG_xuqVYDWbWEmtwLuJuwGML2MAtOL_pYRj3Du37jYO_3TjNOe2DH1SYsAojJDFdBBXhbG9ToYt-SCnlpthF3Ex7Yg9j602c49b5HdbT6NPfDEn8H7-v1stfOCYyvEFHVrkIb-_1BP28OF-vPmeX3z59WS0vM81IIbPKNpRak1c810KWrJRGQ5LGcNHYhlQFN5YxraAxVlgNSjBFBTRCp7qYYSfow4G7Cf5mC3Gs-y7OLagB_DbWtKRCMs5Jkaz8YNXBxxjA1pvQ9amBmpJ6XqZOy9RPl0mx9_cXtk0P5iH0f4pkqA6GnXepyXjttjsIdQvKje3z7DsPqZzA</recordid><startdate>201511</startdate><enddate>201511</enddate><creator>Kim, Ju-Dong</creator><creator>Lee, Seung-Hyun</creator><creator>Seo, Eun-Hye</creator><creator>Woo, So-Yon</creator><creator>Kim, Sung-Hea</creator><creator>Chung, Sang-Man</creator><creator>Kim, Hyun-Joong</creator><general>Copyright Wolters Kluwer Health, Inc. All rights reserved</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201511</creationdate><title>Role of Th1 and Th17 cells in the development and complexity of coronary artery disease: comparison analysis by the methods of flow cytometry and SYNTAX score</title><author>Kim, Ju-Dong ; Lee, Seung-Hyun ; Seo, Eun-Hye ; Woo, So-Yon ; Kim, Sung-Hea ; Chung, Sang-Man ; Kim, Hyun-Joong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3059-8fb11fd2842c697379dce737bd46bfb0854df33caebdf6fcea63a16eb6c4733d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Aged</topic><topic>Angina, Stable - diagnostic imaging</topic><topic>Angina, Stable - immunology</topic><topic>Coronary Angiography</topic><topic>Coronary Artery Disease - diagnostic imaging</topic><topic>Coronary Artery Disease - immunology</topic><topic>Female</topic><topic>Flow Cytometry</topic><topic>Humans</topic><topic>Interferon-gamma - immunology</topic><topic>Interleukin-17 - immunology</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Myocardial Infarction - diagnostic imaging</topic><topic>Myocardial Infarction - immunology</topic><topic>Severity of Illness Index</topic><topic>Th1 Cells - immunology</topic><topic>Th17 Cells - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Ju-Dong</creatorcontrib><creatorcontrib>Lee, Seung-Hyun</creatorcontrib><creatorcontrib>Seo, Eun-Hye</creatorcontrib><creatorcontrib>Woo, So-Yon</creatorcontrib><creatorcontrib>Kim, Sung-Hea</creatorcontrib><creatorcontrib>Chung, Sang-Man</creatorcontrib><creatorcontrib>Kim, Hyun-Joong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Coronary artery disease</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Ju-Dong</au><au>Lee, Seung-Hyun</au><au>Seo, Eun-Hye</au><au>Woo, So-Yon</au><au>Kim, Sung-Hea</au><au>Chung, Sang-Man</au><au>Kim, Hyun-Joong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Role of Th1 and Th17 cells in the development and complexity of coronary artery disease: comparison analysis by the methods of flow cytometry and SYNTAX score</atitle><jtitle>Coronary artery disease</jtitle><addtitle>Coron Artery Dis</addtitle><date>2015-11</date><risdate>2015</risdate><volume>26</volume><issue>7</issue><spage>604</spage><epage>611</epage><pages>604-611</pages><issn>0954-6928</issn><eissn>1473-5830</eissn><abstract>BACKGROUNDThere have been few reports on the relationship between the expression of the CD4 T cells producing interferon-γ (Th1)/interleukin-17 (Th17) and degree of atherosclerosis. Thus, we analyzed Th1 and Th17 cell frequencies in patients with noncardiac chest pain (control), stable angina (SA), and acute myocardial infarction (AMI), and compared the complexity of the coronary artery with the SYNTAX score.
PATIENTS AND METHODSThis study included 124 patients with a complaint of chest pain who underwent coronary angiography (control30 patients, SA47 patients, AMI47 patients). Peripheral blood was sampled during coronary angiography. Mononuclear cells from patients were stimulated for 4 h ex vivo. After staining with specific antibodies and fluorescence, the frequencies of Th1 and Th17 cells were measured by flow cytometry. The SYNTAX score was calculated by coronary angiography and a web-based calculator.
RESULTSThere was no significant difference in the baseline characteristics, except the higher frequencies of hypertension in SA patients (76.1%) and smoking in AMI patients (53.3%). Patients with SA showed a significantly higher frequency of Th1 cells (21.56±9.57%) compared with controls (14.84±8.58%) and patients with AMI (9.04±7.02%) (P<0.001). The frequency of Th17 cells also increased in SA patients (control1.90±1.05%, SA2.96±1.42%, AMI1.32±0.92%, P<0.001). The SYNTAX score was significantly higher in SA patients (SA21.51±11.67, AMI15.36±8.84, P=0.006) and correlated with the frequencies of Th1 and Th17 cells (r=0.359, P=0.001; r=0.248, P=0.031; respectively).
CONCLUSIONTh1 and Th17 cells were related to the development of SA, but not AMI. They could be a useful marker for the complexity of atherosclerosis in coronary artery disease.</abstract><cop>England</cop><pub>Copyright Wolters Kluwer Health, Inc. All rights reserved</pub><pmid>26180995</pmid><doi>10.1097/MCA.0000000000000289</doi><tpages>8</tpages></addata></record> |
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| subjects | Aged Angina, Stable - diagnostic imaging Angina, Stable - immunology Coronary Angiography Coronary Artery Disease - diagnostic imaging Coronary Artery Disease - immunology Female Flow Cytometry Humans Interferon-gamma - immunology Interleukin-17 - immunology Male Middle Aged Myocardial Infarction - diagnostic imaging Myocardial Infarction - immunology Severity of Illness Index Th1 Cells - immunology Th17 Cells - immunology |
| title | Role of Th1 and Th17 cells in the development and complexity of coronary artery disease: comparison analysis by the methods of flow cytometry and SYNTAX score |
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