miR-214 promotes osteosarcoma tumor growth and metastasis by decreasing the expression of PTEN
MicroRNAs (miRNAs) are a class of small non-coding RNAs, which function as critical gene regulators by targeting mRNAs for translational repression or degradation, and they are essential in cancer development and progression. Several previous studies have indicated that abnormal expression of miRNAs...
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| Veröffentlicht in: | Molecular medicine reports 2015-10, Vol.12 (4), p.6261-6266 |
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| description | MicroRNAs (miRNAs) are a class of small non-coding RNAs, which function as critical gene regulators by targeting mRNAs for translational repression or degradation, and they are essential in cancer development and progression. Several previous studies have indicated that abnormal expression of miRNAs occurs frequently in human osteosarcoma (OS) tissues compared with that of adjacent normal tissues. In the present study, the role of miR-214 in the progression and metastasis of OS was investigated. The expression of miR-214 was frequently increased in OS tissues and cell lines. Inhibition of miR-214 in OS cell lines markedly suppressed cell proliferation, migration and invasion. Phosphatase and tensin homolog (PTEN) was identified as a direct target of miR-214, and ectopic expression of miR-214 inhibited PTEN by directly binding to its 3′-untranslated region. The expression of miR-214 negatively correlated with PTEN in OS tissues. Together, these data indicated that miR-214 acts as an oncogenic miRNA and may contribute to the progression, and metastasis of OS, suggesting that miR-214 may be a potential novel diagnostic and therapeutic target of OS. |
| doi_str_mv | 10.3892/mmr.2015.4197 |
| format | Article |
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Several previous studies have indicated that abnormal expression of miRNAs occurs frequently in human osteosarcoma (OS) tissues compared with that of adjacent normal tissues. In the present study, the role of miR-214 in the progression and metastasis of OS was investigated. The expression of miR-214 was frequently increased in OS tissues and cell lines. Inhibition of miR-214 in OS cell lines markedly suppressed cell proliferation, migration and invasion. Phosphatase and tensin homolog (PTEN) was identified as a direct target of miR-214, and ectopic expression of miR-214 inhibited PTEN by directly binding to its 3′-untranslated region. The expression of miR-214 negatively correlated with PTEN in OS tissues. Together, these data indicated that miR-214 acts as an oncogenic miRNA and may contribute to the progression, and metastasis of OS, suggesting that miR-214 may be a potential novel diagnostic and therapeutic target of OS.</description><identifier>ISSN: 1791-2997</identifier><identifier>EISSN: 1791-3004</identifier><identifier>DOI: 10.3892/mmr.2015.4197</identifier><identifier>PMID: 26252022</identifier><language>eng</language><publisher>Greece: D.A. Spandidos</publisher><subject>3' Untranslated Regions ; Apoptosis ; Bone cancer ; Cancer ; Cancer therapies ; Cell cycle ; Cell growth ; Cell Line, Tumor ; Cell Movement ; Cell Proliferation ; Cell Survival ; Comparative analysis ; Ectopic expression ; Gene expression ; Gene Expression Regulation, Neoplastic ; Genetic aspects ; Humans ; Metastases ; Metastasis ; MicroRNA ; MicroRNAs - genetics ; MicroRNAs - metabolism ; miR-214 ; miRNA ; Mutation ; Osteosarcoma ; Osteosarcoma - pathology ; Penicillin ; phosphatase and tensin homolog ; Physiological aspects ; Protein expression ; Proteins ; PTEN Phosphohydrolase - genetics ; PTEN Phosphohydrolase - metabolism ; PTEN protein ; Risk factors ; Rodents ; Sarcoma ; Software ; Statistics ; Studies ; Tensin ; Tumorigenesis ; Tumors</subject><ispartof>Molecular medicine reports, 2015-10, Vol.12 (4), p.6261-6266</ispartof><rights>Copyright: © Liu.</rights><rights>COPYRIGHT 2015 Spandidos Publications</rights><rights>Copyright Spandidos Publications UK Ltd. 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c459t-9d731f3a47525246adbe7f51c5846fad594d944fb3edd25d78d9c926a8bf75543</citedby><cites>FETCH-LOGICAL-c459t-9d731f3a47525246adbe7f51c5846fad594d944fb3edd25d78d9c926a8bf75543</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,5573,27929,27930</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26252022$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>LIU, CHUN-JIE</creatorcontrib><creatorcontrib>YU, KUN-LUN</creatorcontrib><creatorcontrib>LIU, GUO-LI</creatorcontrib><creatorcontrib>TIAN, DE-HU</creatorcontrib><title>miR-214 promotes osteosarcoma tumor growth and metastasis by decreasing the expression of PTEN</title><title>Molecular medicine reports</title><addtitle>Mol Med Rep</addtitle><description>MicroRNAs (miRNAs) are a class of small non-coding RNAs, which function as critical gene regulators by targeting mRNAs for translational repression or degradation, and they are essential in cancer development and progression. Several previous studies have indicated that abnormal expression of miRNAs occurs frequently in human osteosarcoma (OS) tissues compared with that of adjacent normal tissues. In the present study, the role of miR-214 in the progression and metastasis of OS was investigated. The expression of miR-214 was frequently increased in OS tissues and cell lines. Inhibition of miR-214 in OS cell lines markedly suppressed cell proliferation, migration and invasion. Phosphatase and tensin homolog (PTEN) was identified as a direct target of miR-214, and ectopic expression of miR-214 inhibited PTEN by directly binding to its 3′-untranslated region. The expression of miR-214 negatively correlated with PTEN in OS tissues. Together, these data indicated that miR-214 acts as an oncogenic miRNA and may contribute to the progression, and metastasis of OS, suggesting that miR-214 may be a potential novel diagnostic and therapeutic target of OS.</description><subject>3' Untranslated Regions</subject><subject>Apoptosis</subject><subject>Bone cancer</subject><subject>Cancer</subject><subject>Cancer therapies</subject><subject>Cell cycle</subject><subject>Cell growth</subject><subject>Cell Line, Tumor</subject><subject>Cell Movement</subject><subject>Cell Proliferation</subject><subject>Cell Survival</subject><subject>Comparative analysis</subject><subject>Ectopic expression</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Genetic aspects</subject><subject>Humans</subject><subject>Metastases</subject><subject>Metastasis</subject><subject>MicroRNA</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><subject>miR-214</subject><subject>miRNA</subject><subject>Mutation</subject><subject>Osteosarcoma</subject><subject>Osteosarcoma - pathology</subject><subject>Penicillin</subject><subject>phosphatase and tensin homolog</subject><subject>Physiological aspects</subject><subject>Protein expression</subject><subject>Proteins</subject><subject>PTEN Phosphohydrolase - genetics</subject><subject>PTEN Phosphohydrolase - metabolism</subject><subject>PTEN protein</subject><subject>Risk factors</subject><subject>Rodents</subject><subject>Sarcoma</subject><subject>Software</subject><subject>Statistics</subject><subject>Studies</subject><subject>Tensin</subject><subject>Tumorigenesis</subject><subject>Tumors</subject><issn>1791-2997</issn><issn>1791-3004</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNptkc9vFCEUxydGY2v16NWQeNDLrPCAYTk2Tf2RNGpMvUqY4bGlWYYVmGj_e9nsWqMxkPAgn_fyJZ-ue87oiq81vIkxr4AyuRJMqwfdKVOa9ZxS8fBYg9bqpHtSyi2lgwSpH3cnMIAECnDafYvhSw9MkF1OMVUsJJWKqdg8pWhJXWLKZJPTj3pD7OxIxGpL26GQ8Y44nDK2y7wh9QYJ_txlLCWkmSRPPl9ffnzaPfJ2W_DZ8Tzrvr69vL543199evfh4vyqn4TUtddOcea5FaoFBDFYN6Lykk1yLQZvndTCaSH8yNE5kE6tnZ40DHY9eiWl4Gfd68Pc9o3vC5ZqYigTbrd2xrQUwxQbNAfQvKEv_0Fv05Lnls6whrQIAtgfamO3aMLsU8122g8156JRWqpBNmr1H6othzFMaUYf2vtfDf2hYcqplIze7HKINt8ZRs3ep2k-zd6n2fts_Itj2GWM6O7p3wIb8OoAlF3TE1wq90yb1DPoqegHGBj_BQF-pkg</recordid><startdate>20151001</startdate><enddate>20151001</enddate><creator>LIU, CHUN-JIE</creator><creator>YU, KUN-LUN</creator><creator>LIU, GUO-LI</creator><creator>TIAN, DE-HU</creator><general>D.A. Spandidos</general><general>Spandidos Publications</general><general>Spandidos Publications UK Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20151001</creationdate><title>miR-214 promotes osteosarcoma tumor growth and metastasis by decreasing the expression of PTEN</title><author>LIU, CHUN-JIE ; YU, KUN-LUN ; LIU, GUO-LI ; TIAN, DE-HU</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c459t-9d731f3a47525246adbe7f51c5846fad594d944fb3edd25d78d9c926a8bf75543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>3' Untranslated Regions</topic><topic>Apoptosis</topic><topic>Bone cancer</topic><topic>Cancer</topic><topic>Cancer therapies</topic><topic>Cell cycle</topic><topic>Cell growth</topic><topic>Cell Line, Tumor</topic><topic>Cell Movement</topic><topic>Cell Proliferation</topic><topic>Cell Survival</topic><topic>Comparative analysis</topic><topic>Ectopic expression</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Genetic aspects</topic><topic>Humans</topic><topic>Metastases</topic><topic>Metastasis</topic><topic>MicroRNA</topic><topic>MicroRNAs - genetics</topic><topic>MicroRNAs - metabolism</topic><topic>miR-214</topic><topic>miRNA</topic><topic>Mutation</topic><topic>Osteosarcoma</topic><topic>Osteosarcoma - pathology</topic><topic>Penicillin</topic><topic>phosphatase and tensin homolog</topic><topic>Physiological aspects</topic><topic>Protein expression</topic><topic>Proteins</topic><topic>PTEN Phosphohydrolase - genetics</topic><topic>PTEN Phosphohydrolase - metabolism</topic><topic>PTEN protein</topic><topic>Risk factors</topic><topic>Rodents</topic><topic>Sarcoma</topic><topic>Software</topic><topic>Statistics</topic><topic>Studies</topic><topic>Tensin</topic><topic>Tumorigenesis</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>LIU, CHUN-JIE</creatorcontrib><creatorcontrib>YU, KUN-LUN</creatorcontrib><creatorcontrib>LIU, GUO-LI</creatorcontrib><creatorcontrib>TIAN, DE-HU</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>British Nursing Database</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection (ProQuest)</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular medicine reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>LIU, CHUN-JIE</au><au>YU, KUN-LUN</au><au>LIU, GUO-LI</au><au>TIAN, DE-HU</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>miR-214 promotes osteosarcoma tumor growth and metastasis by decreasing the expression of PTEN</atitle><jtitle>Molecular medicine reports</jtitle><addtitle>Mol Med Rep</addtitle><date>2015-10-01</date><risdate>2015</risdate><volume>12</volume><issue>4</issue><spage>6261</spage><epage>6266</epage><pages>6261-6266</pages><issn>1791-2997</issn><eissn>1791-3004</eissn><abstract>MicroRNAs (miRNAs) are a class of small non-coding RNAs, which function as critical gene regulators by targeting mRNAs for translational repression or degradation, and they are essential in cancer development and progression. Several previous studies have indicated that abnormal expression of miRNAs occurs frequently in human osteosarcoma (OS) tissues compared with that of adjacent normal tissues. In the present study, the role of miR-214 in the progression and metastasis of OS was investigated. The expression of miR-214 was frequently increased in OS tissues and cell lines. Inhibition of miR-214 in OS cell lines markedly suppressed cell proliferation, migration and invasion. Phosphatase and tensin homolog (PTEN) was identified as a direct target of miR-214, and ectopic expression of miR-214 inhibited PTEN by directly binding to its 3′-untranslated region. The expression of miR-214 negatively correlated with PTEN in OS tissues. Together, these data indicated that miR-214 acts as an oncogenic miRNA and may contribute to the progression, and metastasis of OS, suggesting that miR-214 may be a potential novel diagnostic and therapeutic target of OS.</abstract><cop>Greece</cop><pub>D.A. Spandidos</pub><pmid>26252022</pmid><doi>10.3892/mmr.2015.4197</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | Spandidos Publications Journals; MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | 3' Untranslated Regions Apoptosis Bone cancer Cancer Cancer therapies Cell cycle Cell growth Cell Line, Tumor Cell Movement Cell Proliferation Cell Survival Comparative analysis Ectopic expression Gene expression Gene Expression Regulation, Neoplastic Genetic aspects Humans Metastases Metastasis MicroRNA MicroRNAs - genetics MicroRNAs - metabolism miR-214 miRNA Mutation Osteosarcoma Osteosarcoma - pathology Penicillin phosphatase and tensin homolog Physiological aspects Protein expression Proteins PTEN Phosphohydrolase - genetics PTEN Phosphohydrolase - metabolism PTEN protein Risk factors Rodents Sarcoma Software Statistics Studies Tensin Tumorigenesis Tumors |
| title | miR-214 promotes osteosarcoma tumor growth and metastasis by decreasing the expression of PTEN |
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