Interassay Correlation of Human Herpesvirus 8 Serologic Tests
To standardize human herpesvirus 8 (HHV-8) antibody assays for application to asymptomatic infection, a blinded comparison was done of seven immunofluorescence assays and ELISAs. Five experienced laboratories tested a serum panel from 143 subjects in 4 diagnostic groups. Except for a minor capsid pr...
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| Veröffentlicht in: | The Journal of infectious diseases 1998-08, Vol.178 (2), p.304-309 |
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| container_issue | 2 |
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| container_title | The Journal of infectious diseases |
| container_volume | 178 |
| creator | Rabkin, Charles S. Schulz, Thomas F. Whitby, Denise Lennette, Evelyne T. Magpantay, Larry I. Chatlynne, Louise Biggar, Robert J. |
| description | To standardize human herpesvirus 8 (HHV-8) antibody assays for application to asymptomatic infection, a blinded comparison was done of seven immunofluorescence assays and ELISAs. Five experienced laboratories tested a serum panel from 143 subjects in 4 diagnostic groups. Except for a minor capsid protein ELISA, the other six tests detected HHV-8 antibodies most frequently in classic (80%–100%) and AIDS-related (67%–91%) Kaposi's sarcoma, followed by human immunodeficiency virus-seropositive patients (27%–60%), and least frequently in healthy blood donors (0–29%). However, these six assays frequently disagreed on individual sera, particularly for blood donor samples. Current HHV-8 antibody tests have uncertain accuracy in asymptomatic HHV-8 infection and may require correlation with viral protein or nucleic acid detection. Antibody assays are useful for epidemiologic investigations, but the absolute prevalence of HHV-8 infection in the United States cannot yet be determined. |
| doi_str_mv | 10.1086/515649 |
| format | Article |
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Five experienced laboratories tested a serum panel from 143 subjects in 4 diagnostic groups. Except for a minor capsid protein ELISA, the other six tests detected HHV-8 antibodies most frequently in classic (80%–100%) and AIDS-related (67%–91%) Kaposi's sarcoma, followed by human immunodeficiency virus-seropositive patients (27%–60%), and least frequently in healthy blood donors (0–29%). However, these six assays frequently disagreed on individual sera, particularly for blood donor samples. Current HHV-8 antibody tests have uncertain accuracy in asymptomatic HHV-8 infection and may require correlation with viral protein or nucleic acid detection. 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Psychology</subject><subject>Herpesviridae</subject><subject>Human herpesvirus 8</subject><subject>Human viral diseases</subject><subject>Infectious diseases</subject><subject>Kaposi sarcoma</subject><subject>Major Articles</subject><subject>Medical sciences</subject><subject>Microbiology</subject><subject>Open reading frames</subject><subject>Techniques used in virology</subject><subject>Viral diseases</subject><subject>Viral diseases with cutaneous or mucosal lesions and viral diseases of the eye</subject><subject>Virology</subject><subject>Viruses</subject><issn>0022-1899</issn><issn>1537-6613</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><recordid>eNpdkEuL1EAUhQtRsB31HwhBZHbRW--qhQttRjPYMIsZH7gpKulbkjadausm4vx7M_TQgqu7OB_fuRzGnnN4zcGZN5pro_wDtuJa2toYLh-yFYAQNXfeP2ZPiHYAoKSxK_b2cpywRKJ4W61zKTjEqc9jlVPVzPs4Vg2WA9LvvsxUueoaSx7yj76rbpAmesoepTgQPru_Z-zzh4ubdVNvrj5ert9t6k5JNdUe27ZFk4wTynbet87CNhnkVmGbktY-RQQrQHoQTju3RS48OAm-23K5lWfs_Og9lPxrXprDvqcOhyGOmGcK3HLttVML-PI_cJfnMi6_BSEWuwcr_9m6kokKpnAo_T6W28Ah3E0YjhMu4Kt7W6QuDqnEsevpRAupBBi9YC-O2I6mXE6xBM69Vnd99THvacI_pzyWn8FYaXVovn0Pm0_SvPdfvoZG_gWuhoZY</recordid><startdate>19980801</startdate><enddate>19980801</enddate><creator>Rabkin, Charles S.</creator><creator>Schulz, Thomas F.</creator><creator>Whitby, Denise</creator><creator>Lennette, Evelyne T.</creator><creator>Magpantay, Larry I.</creator><creator>Chatlynne, Louise</creator><creator>Biggar, Robert J.</creator><general>The University of Chicago Press</general><general>University of Chicago Press</general><general>Oxford University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>7U9</scope><scope>H94</scope></search><sort><creationdate>19980801</creationdate><title>Interassay Correlation of Human Herpesvirus 8 Serologic Tests</title><author>Rabkin, Charles S. ; Schulz, Thomas F. ; Whitby, Denise ; Lennette, Evelyne T. ; Magpantay, Larry I. ; Chatlynne, Louise ; Biggar, Robert J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c434t-9ebbbe6f68247c99b870df6e174ebff559fae072039028588de12908309cd13d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Antibodies</topic><topic>Antigens</topic><topic>Biological and medical sciences</topic><topic>Blood donation</topic><topic>Capsid proteins</topic><topic>Enzyme linked immunosorbent assay</topic><topic>Fundamental and applied biological sciences. 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| ispartof | The Journal of infectious diseases, 1998-08, Vol.178 (2), p.304-309 |
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| source | JSTOR Archive Collection A-Z Listing |
| subjects | Antibodies Antigens Biological and medical sciences Blood donation Capsid proteins Enzyme linked immunosorbent assay Fundamental and applied biological sciences. Psychology Herpesviridae Human herpesvirus 8 Human viral diseases Infectious diseases Kaposi sarcoma Major Articles Medical sciences Microbiology Open reading frames Techniques used in virology Viral diseases Viral diseases with cutaneous or mucosal lesions and viral diseases of the eye Virology Viruses |
| title | Interassay Correlation of Human Herpesvirus 8 Serologic Tests |
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