Efficient segmental isotope labeling of multi-domain proteins using Sortase A

NMR studies of multi-domain protein complexes provide unique insight into their molecular interactions and dynamics in solution. For large proteins domain-selective isotope labeling is desired to reduce signal overlap, but available methods require extensive optimization and often give poor ligation...

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Veröffentlicht in:	Journal of biomolecular NMR 2015-09, Vol.63 (1), p.1-8
Hauptverfasser:	Freiburger, Lee, Sonntag, Miriam, Hennig, Janosch, Li, Jian, Zou, Peijian, Sattler, Michael
Format:	Artikel
Sprache:	eng
Schlagworte:	Aminoacyltransferases - metabolism Bacterial Proteins - metabolism Biochemistry Biological and Medical Physics Biophysics Communication Cysteine Endopeptidases - metabolism Electrophoresis, Polyacrylamide Gel Isotope Labeling - methods Physics Physics and Astronomy Protein Structure, Tertiary Proton Magnetic Resonance Spectroscopy Spectroscopy/Spectrometry Staphylococcus aureus - enzymology
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