Improved Antiserum for Sensitive Serologic Detection of Chickpea chlorotic dwarf virus

A Syrian chickpea isolate of Chickpea chlorotic dwarf virus (CpCDV; genus Mastrevirus, family Geminiviridae) was purified and yielded 0.6-0.8 mg of purified virus per kg of infected chickpea tissue. The purified preparations were injected into a rabbit and an antiserum of good quality was obtained a...

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Veröffentlicht in:	Journal of phytopathology 2006-03, Vol.154 (3), p.129-133
Hauptverfasser:	Kumari, S.G, Makkouk, K.M, Attar, N
Format:	Artikel
Sprache:	eng
Schlagworte:	antiserum Biological and medical sciences Chickpea chlorotic dwarf virus chickpeas Cicer arietinum coat proteins diagnosis direct antigen-coating enzyme-linked immunosorbent assay disease detection disease diagnosis dot-blot double-antibody sandwich enzyme-linked immunosorbent assay enzyme-linked immunosorbent assay Fundamental and applied biological sciences. Psychology Geminivirus immunologic techniques leaves Mastrevirus new methods Phytopathology. Animal pests. Plant and forest protection plant extracts plant viruses Plant viruses and viroids sap serodiagnosis tissue-blot immunoassay Western blot
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