Sex-determining region Y box 4 is a transforming oncogene in human prostate cancer cells
Prostate cancer is the most commonly diagnosed noncutaneous neoplasm and second most common cause of cancer-related mortality in western men. To investigate the mechanisms of prostate cancer development and progression, we did expression profiling of human prostate cancer and benign tissues. We show...
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| Veröffentlicht in: | Cancer research (Chicago, Ill.) Ill.), 2006-04, Vol.66 (8), p.4011-4019 |
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| creator | PENGBO LIU RAMACHANDRAN, Sumathi MORENO, Carlos S SEYED, Mohamed Ali SCHARER, Christopher D LAYCOCK, Noelani DALTON, W. Brian WILLIAMS, Holly KARANAM, Suresh DATTA, Milton W JAYE, David L |
| description | Prostate cancer is the most commonly diagnosed noncutaneous neoplasm and second most common cause of cancer-related mortality in western men. To investigate the mechanisms of prostate cancer development and progression, we did expression profiling of human prostate cancer and benign tissues. We show that the SOX4 is overexpressed in prostate tumor samples compared with benign tissues by microarray analysis, real-time PCR, and immunohistochemistry. We also show that SOX4 expression is highly correlated with Gleason score at the mRNA and protein level using tissue microarrays. Genes affected by SOX4 expression were also identified, including BCL10, CSF1, and NcoA4/ARA70. TLE-1 and BBC3/PUMA were identified as direct targets of SOX4. Silencing of SOX4 by small interfering RNA transfection induced apoptosis of prostate cancer cells, suggesting that SOX4 could be a therapeutic target for prostate cancer. Stable transfection of SOX4 into nontransformed prostate cells enabled colony formation in soft agar, suggesting that, in the proper cellular context, SOX4 can be a transforming oncogene. |
| doi_str_mv | 10.1158/0008-5472.CAN-05-3055 |
| format | Article |
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Brian ; WILLIAMS, Holly ; KARANAM, Suresh ; DATTA, Milton W ; JAYE, David L</creator><creatorcontrib>PENGBO LIU ; RAMACHANDRAN, Sumathi ; MORENO, Carlos S ; SEYED, Mohamed Ali ; SCHARER, Christopher D ; LAYCOCK, Noelani ; DALTON, W. Brian ; WILLIAMS, Holly ; KARANAM, Suresh ; DATTA, Milton W ; JAYE, David L</creatorcontrib><description>Prostate cancer is the most commonly diagnosed noncutaneous neoplasm and second most common cause of cancer-related mortality in western men. To investigate the mechanisms of prostate cancer development and progression, we did expression profiling of human prostate cancer and benign tissues. We show that the SOX4 is overexpressed in prostate tumor samples compared with benign tissues by microarray analysis, real-time PCR, and immunohistochemistry. We also show that SOX4 expression is highly correlated with Gleason score at the mRNA and protein level using tissue microarrays. Genes affected by SOX4 expression were also identified, including BCL10, CSF1, and NcoA4/ARA70. TLE-1 and BBC3/PUMA were identified as direct targets of SOX4. Silencing of SOX4 by small interfering RNA transfection induced apoptosis of prostate cancer cells, suggesting that SOX4 could be a therapeutic target for prostate cancer. Stable transfection of SOX4 into nontransformed prostate cells enabled colony formation in soft agar, suggesting that, in the proper cellular context, SOX4 can be a transforming oncogene.</description><identifier>ISSN: 0008-5472</identifier><identifier>EISSN: 1538-7445</identifier><identifier>DOI: 10.1158/0008-5472.CAN-05-3055</identifier><identifier>PMID: 16618720</identifier><identifier>CODEN: CNREA8</identifier><language>eng</language><publisher>Philadelphia, PA: American Association for Cancer Research</publisher><subject>Antineoplastic agents ; Apoptosis - genetics ; Biological and medical sciences ; Cell Growth Processes - physiology ; Cell Line, Transformed ; Cell Line, Tumor ; Cell Transformation, Neoplastic - genetics ; Cell Transformation, Neoplastic - metabolism ; Gene Expression Profiling ; Gynecology. Andrology. Obstetrics ; High Mobility Group Proteins - biosynthesis ; High Mobility Group Proteins - genetics ; Humans ; Male ; Male genital diseases ; Medical sciences ; Nephrology. Urinary tract diseases ; Oncogenes ; Pharmacology. Drug treatments ; Prostatic Neoplasms - genetics ; Prostatic Neoplasms - metabolism ; Prostatic Neoplasms - pathology ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Small Interfering - genetics ; SOXC Transcription Factors ; Trans-Activators - biosynthesis ; Trans-Activators - genetics ; Transfection ; Tumors ; Tumors of the urinary system ; Urinary tract. Prostate gland</subject><ispartof>Cancer research (Chicago, Ill.), 2006-04, Vol.66 (8), p.4011-4019</ispartof><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c566t-a45ba5ef6a6c2ee1ee3ef5e930905fa6613024c0de696d4d75703ea5c30248e23</citedby><cites>FETCH-LOGICAL-c566t-a45ba5ef6a6c2ee1ee3ef5e930905fa6613024c0de696d4d75703ea5c30248e23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3343,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17699904$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16618720$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>PENGBO LIU</creatorcontrib><creatorcontrib>RAMACHANDRAN, Sumathi</creatorcontrib><creatorcontrib>MORENO, Carlos S</creatorcontrib><creatorcontrib>SEYED, Mohamed Ali</creatorcontrib><creatorcontrib>SCHARER, Christopher D</creatorcontrib><creatorcontrib>LAYCOCK, Noelani</creatorcontrib><creatorcontrib>DALTON, W. Brian</creatorcontrib><creatorcontrib>WILLIAMS, Holly</creatorcontrib><creatorcontrib>KARANAM, Suresh</creatorcontrib><creatorcontrib>DATTA, Milton W</creatorcontrib><creatorcontrib>JAYE, David L</creatorcontrib><title>Sex-determining region Y box 4 is a transforming oncogene in human prostate cancer cells</title><title>Cancer research (Chicago, Ill.)</title><addtitle>Cancer Res</addtitle><description>Prostate cancer is the most commonly diagnosed noncutaneous neoplasm and second most common cause of cancer-related mortality in western men. To investigate the mechanisms of prostate cancer development and progression, we did expression profiling of human prostate cancer and benign tissues. We show that the SOX4 is overexpressed in prostate tumor samples compared with benign tissues by microarray analysis, real-time PCR, and immunohistochemistry. We also show that SOX4 expression is highly correlated with Gleason score at the mRNA and protein level using tissue microarrays. Genes affected by SOX4 expression were also identified, including BCL10, CSF1, and NcoA4/ARA70. TLE-1 and BBC3/PUMA were identified as direct targets of SOX4. Silencing of SOX4 by small interfering RNA transfection induced apoptosis of prostate cancer cells, suggesting that SOX4 could be a therapeutic target for prostate cancer. Stable transfection of SOX4 into nontransformed prostate cells enabled colony formation in soft agar, suggesting that, in the proper cellular context, SOX4 can be a transforming oncogene.</description><subject>Antineoplastic agents</subject><subject>Apoptosis - genetics</subject><subject>Biological and medical sciences</subject><subject>Cell Growth Processes - physiology</subject><subject>Cell Line, Transformed</subject><subject>Cell Line, Tumor</subject><subject>Cell Transformation, Neoplastic - genetics</subject><subject>Cell Transformation, Neoplastic - metabolism</subject><subject>Gene Expression Profiling</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>High Mobility Group Proteins - biosynthesis</subject><subject>High Mobility Group Proteins - genetics</subject><subject>Humans</subject><subject>Male</subject><subject>Male genital diseases</subject><subject>Medical sciences</subject><subject>Nephrology. Urinary tract diseases</subject><subject>Oncogenes</subject><subject>Pharmacology. Drug treatments</subject><subject>Prostatic Neoplasms - genetics</subject><subject>Prostatic Neoplasms - metabolism</subject><subject>Prostatic Neoplasms - pathology</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Small Interfering - genetics</subject><subject>SOXC Transcription Factors</subject><subject>Trans-Activators - biosynthesis</subject><subject>Trans-Activators - genetics</subject><subject>Transfection</subject><subject>Tumors</subject><subject>Tumors of the urinary system</subject><subject>Urinary tract. Prostate gland</subject><issn>0008-5472</issn><issn>1538-7445</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkE1PwzAMhiMEYmPwE0C5wK3DaeO0PU4TX9IEB0CCU5Sl7iha09G00vj3pNrETpatx_arh7FLAVMhMLsFgCxCmcbT-ew5AowSQDxiY4FJFqVS4jEb_zMjdub9d2hRAJ6ykVBKZGkMY_bxStuooI7aunKVW_GWVlXj-CdfNlsueeW54V1rnC-bAVnxxtlmRY545fhXXxvHN23jO9MRt8ZZarml9dqfs5PSrD1d7OuEvd_fvc0fo8XLw9N8togsKtVFRuLSIJXKKBsTCaKESqQ8gRywNCFnArG0UJDKVSGLFFNIyKAdxhnFyYTd7O6GFD89-U7XlR8SGEdN77VIhcRMQQBxB9oQ17dU6k1b1ab91QL0oFQPuvSgSwelGlAPSsPe1f5Bv6ypOGztHQbgeg8Yb826DLJs5Q9cqvI8B5n8AeOIfuk</recordid><startdate>20060415</startdate><enddate>20060415</enddate><creator>PENGBO LIU</creator><creator>RAMACHANDRAN, Sumathi</creator><creator>MORENO, Carlos S</creator><creator>SEYED, Mohamed Ali</creator><creator>SCHARER, Christopher D</creator><creator>LAYCOCK, Noelani</creator><creator>DALTON, W. 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| ispartof | Cancer research (Chicago, Ill.), 2006-04, Vol.66 (8), p.4011-4019 |
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| language | eng |
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| source | MEDLINE; American Association for Cancer Research; EZB-FREE-00999 freely available EZB journals |
| subjects | Antineoplastic agents Apoptosis - genetics Biological and medical sciences Cell Growth Processes - physiology Cell Line, Transformed Cell Line, Tumor Cell Transformation, Neoplastic - genetics Cell Transformation, Neoplastic - metabolism Gene Expression Profiling Gynecology. Andrology. Obstetrics High Mobility Group Proteins - biosynthesis High Mobility Group Proteins - genetics Humans Male Male genital diseases Medical sciences Nephrology. Urinary tract diseases Oncogenes Pharmacology. Drug treatments Prostatic Neoplasms - genetics Prostatic Neoplasms - metabolism Prostatic Neoplasms - pathology Reverse Transcriptase Polymerase Chain Reaction RNA, Small Interfering - genetics SOXC Transcription Factors Trans-Activators - biosynthesis Trans-Activators - genetics Transfection Tumors Tumors of the urinary system Urinary tract. Prostate gland |
| title | Sex-determining region Y box 4 is a transforming oncogene in human prostate cancer cells |
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