Construction and sequencing of new derivatives of the pIN-III- ompA secretion vector

Construction and sequencing of new derivatives of the pIN-III- ompA secretion vector

The growing interest in proteins exported by bacteria led to the development of cloning vectors designed for targeting recombinant proteins to extracytoplasmic compartments. The pIN-III- ompA vector family fulfils such a function. Here we report the construction and sequencing of new pIN-III- ompA d...

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Veröffentlicht in:Genetic analysis : biomolecular engineering 1998-10, Vol.14 (4), p.129-131
Hauptverfasser: Fauconnier, Alan, Van Elsen, Ary, Bollen, Alex
Format: Artikel
Sprache:eng
Schlagworte:
Bacterial Outer Membrane Proteins - genetics
Base Sequence
Cloning vector
Genetic Vectors
Molecular Sequence Data
ompA signal peptide
Recombinant Fusion Proteins - genetics
Secretion
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Zusammenfassung:The growing interest in proteins exported by bacteria led to the development of cloning vectors designed for targeting recombinant proteins to extracytoplasmic compartments. The pIN-III- ompA vector family fulfils such a function. Here we report the construction and sequencing of new pIN-III- ompA derivatives with alternate polylinker sites, increasing the cloning flexibility of these vectors.
ISSN:1050-3862
DOI:10.1016/S1050-3862(98)00005-9