Early Diagnosis of Typhoid By PCR For FliC-d Gene of Salmonella Typhi in Patients Taking Antibiotics
To compare PCR (Polymerase Chain Reaction) with blood culture, typhi-dot and Widal test for the diagnosis of typhoid in patients taking antibiotics. Cross-sectional, comparative study. National University of Sciences and Technology, Islamabad, Pakistan, from April 2013 to August 2014. One hundred an...
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| Veröffentlicht in: | Journal of the College of Physicians and Surgeons--Pakistan 2015-09, Vol.25 (9), p.662-666 |
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| container_title | Journal of the College of Physicians and Surgeons--Pakistan |
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| creator | Munir, Tehmina Lodhi, Munir Ali, Salman Hussain Zaidi, Syed Badshah Razak, Suhail |
| description | To compare PCR (Polymerase Chain Reaction) with blood culture, typhi-dot and Widal test for the diagnosis of typhoid in patients taking antibiotics.
Cross-sectional, comparative study.
National University of Sciences and Technology, Islamabad, Pakistan, from April 2013 to August 2014.
One hundred and five patients were included in the study. Blood was collected and inoculated into tryptone soya broth for culture. Any growth obtained was identified by API 20 E and confirmed by Salmonella anti-sera. Typhi-dot and Widal test were also done on all the samples. DNA extraction was done and PCR was carried out.
Among the 105 patients, 79 (75.2%) were males and 26 (24.8%) were females, with mean age of 20.64 ±14 years. Typhi-dot was positive in 58 (55.2%) and negative in 47 (44.8%) patients. Blood widal test was positive in 27 (25.7%) and negative in 78 (74.3%) patients. Salmonella Typhi was positive on blood culture in only one (1%) patient. PCR for Salmonella Typhi was positive in 102 (97.1%) and negative in 3 (2.9%) patients. Positive cases detected by PCR were significantly higher as compared to Typhi-dot (p < 0.001), blood Widal test (p < 0.001) and blood culture (p < 0.001).
Positivity rate of PCR was significantly higher as compared to blood culture, Typhi-dot or Widal test for diagnosing typhoid in patients who were already taking antibiotics. |
| format | Article |
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Cross-sectional, comparative study.
National University of Sciences and Technology, Islamabad, Pakistan, from April 2013 to August 2014.
One hundred and five patients were included in the study. Blood was collected and inoculated into tryptone soya broth for culture. Any growth obtained was identified by API 20 E and confirmed by Salmonella anti-sera. Typhi-dot and Widal test were also done on all the samples. DNA extraction was done and PCR was carried out.
Among the 105 patients, 79 (75.2%) were males and 26 (24.8%) were females, with mean age of 20.64 ±14 years. Typhi-dot was positive in 58 (55.2%) and negative in 47 (44.8%) patients. Blood widal test was positive in 27 (25.7%) and negative in 78 (74.3%) patients. Salmonella Typhi was positive on blood culture in only one (1%) patient. PCR for Salmonella Typhi was positive in 102 (97.1%) and negative in 3 (2.9%) patients. Positive cases detected by PCR were significantly higher as compared to Typhi-dot (p < 0.001), blood Widal test (p < 0.001) and blood culture (p < 0.001).
Positivity rate of PCR was significantly higher as compared to blood culture, Typhi-dot or Widal test for diagnosing typhoid in patients who were already taking antibiotics.</description><identifier>ISSN: 1022-386X</identifier><identifier>EISSN: 1681-7168</identifier><identifier>PMID: 26374362</identifier><language>eng</language><publisher>Pakistan: College of Physicians and Surgeons Pakistan</publisher><subject>Adolescent ; Adult ; Agglutination Tests ; Anti-Bacterial Agents - administration & dosage ; Antibiotics ; Antibodies, Bacterial - blood ; Bacterial Typing Techniques ; Child ; Child, Preschool ; Cross-Sectional Studies ; Early Diagnosis ; Female ; Humans ; Immunoblotting ; Immunoenzyme Techniques ; Immunoglobulin M - blood ; Male ; Pakistan ; Polymerase chain reaction ; Polymerase Chain Reaction - methods ; Predictive Value of Tests ; Reproducibility of Results ; Salmonella typhi - genetics ; Salmonella typhi - immunology ; Salmonella typhi - isolation & purification ; Salmonella typhosa ; Sensitivity and Specificity ; Typhoid fever ; Typhoid Fever - blood ; Typhoid Fever - diagnosis ; Typhoid Fever - drug therapy ; Young Adult</subject><ispartof>Journal of the College of Physicians and Surgeons--Pakistan, 2015-09, Vol.25 (9), p.662-666</ispartof><rights>COPYRIGHT 2015 College of Physicians and Surgeons Pakistan</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26374362$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Munir, Tehmina</creatorcontrib><creatorcontrib>Lodhi, Munir</creatorcontrib><creatorcontrib>Ali, Salman</creatorcontrib><creatorcontrib>Hussain Zaidi, Syed Badshah</creatorcontrib><creatorcontrib>Razak, Suhail</creatorcontrib><title>Early Diagnosis of Typhoid By PCR For FliC-d Gene of Salmonella Typhi in Patients Taking Antibiotics</title><title>Journal of the College of Physicians and Surgeons--Pakistan</title><addtitle>J Coll Physicians Surg Pak</addtitle><description>To compare PCR (Polymerase Chain Reaction) with blood culture, typhi-dot and Widal test for the diagnosis of typhoid in patients taking antibiotics.
Cross-sectional, comparative study.
National University of Sciences and Technology, Islamabad, Pakistan, from April 2013 to August 2014.
One hundred and five patients were included in the study. Blood was collected and inoculated into tryptone soya broth for culture. Any growth obtained was identified by API 20 E and confirmed by Salmonella anti-sera. Typhi-dot and Widal test were also done on all the samples. DNA extraction was done and PCR was carried out.
Among the 105 patients, 79 (75.2%) were males and 26 (24.8%) were females, with mean age of 20.64 ±14 years. Typhi-dot was positive in 58 (55.2%) and negative in 47 (44.8%) patients. Blood widal test was positive in 27 (25.7%) and negative in 78 (74.3%) patients. Salmonella Typhi was positive on blood culture in only one (1%) patient. PCR for Salmonella Typhi was positive in 102 (97.1%) and negative in 3 (2.9%) patients. Positive cases detected by PCR were significantly higher as compared to Typhi-dot (p < 0.001), blood Widal test (p < 0.001) and blood culture (p < 0.001).
Positivity rate of PCR was significantly higher as compared to blood culture, Typhi-dot or Widal test for diagnosing typhoid in patients who were already taking antibiotics.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Agglutination Tests</subject><subject>Anti-Bacterial Agents - administration & dosage</subject><subject>Antibiotics</subject><subject>Antibodies, Bacterial - blood</subject><subject>Bacterial Typing Techniques</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Cross-Sectional Studies</subject><subject>Early Diagnosis</subject><subject>Female</subject><subject>Humans</subject><subject>Immunoblotting</subject><subject>Immunoenzyme Techniques</subject><subject>Immunoglobulin M - blood</subject><subject>Male</subject><subject>Pakistan</subject><subject>Polymerase chain reaction</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Predictive Value of Tests</subject><subject>Reproducibility of Results</subject><subject>Salmonella typhi - genetics</subject><subject>Salmonella typhi - immunology</subject><subject>Salmonella typhi - isolation & purification</subject><subject>Salmonella typhosa</subject><subject>Sensitivity and Specificity</subject><subject>Typhoid fever</subject><subject>Typhoid Fever - blood</subject><subject>Typhoid Fever - diagnosis</subject><subject>Typhoid Fever - drug therapy</subject><subject>Young Adult</subject><issn>1022-386X</issn><issn>1681-7168</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkE1Lw0AQhhdRbK3-BVnw4iWyX9lNj7W2VShYtIK3sNmPuJrs1mx6yL830XoQZGBmGJ55eXmPwBjzDCei78f9jghJaMZfR-AsxneEaIqz7BSMCKeCUU7GQC9kU3XwzsnSh-giDBZuu91bcBrednAzf4LL0MBl5eaJhivjzUA8y6oO3lSV_IYddB5uZOuMbyPcyg_nSzjzrStcaJ2K5-DEyiqai8OcgJflYju_T9aPq4f5bJ2URGRtwlNkkdKKsozxAikriaG6N2qZSQXnhZ7KKUKioFOFJFUIi5RRzLi11BZG0wm4_tHdNeFzb2Kb1y6qwaY3YR9zLDBNCReM9-jVD1rKyuTO29A2Ug14PmMkw4IN8ATc_EP1pU3tVJ-Adf39z8PlwcG-qI3Od42rZdPlv4HTL8Aeeqg</recordid><startdate>20150901</startdate><enddate>20150901</enddate><creator>Munir, Tehmina</creator><creator>Lodhi, Munir</creator><creator>Ali, Salman</creator><creator>Hussain Zaidi, Syed Badshah</creator><creator>Razak, Suhail</creator><general>College of Physicians and Surgeons Pakistan</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20150901</creationdate><title>Early Diagnosis of Typhoid By PCR For FliC-d Gene of Salmonella Typhi in Patients Taking Antibiotics</title><author>Munir, Tehmina ; Lodhi, Munir ; Ali, Salman ; Hussain Zaidi, Syed Badshah ; Razak, Suhail</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-g278t-650f0cdc34846b0cfa2e3d743f4e5766bd9a9007b39c0a3c017543146ff3fbed3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Agglutination Tests</topic><topic>Anti-Bacterial Agents - administration & dosage</topic><topic>Antibiotics</topic><topic>Antibodies, Bacterial - blood</topic><topic>Bacterial Typing Techniques</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Cross-Sectional Studies</topic><topic>Early Diagnosis</topic><topic>Female</topic><topic>Humans</topic><topic>Immunoblotting</topic><topic>Immunoenzyme Techniques</topic><topic>Immunoglobulin M - blood</topic><topic>Male</topic><topic>Pakistan</topic><topic>Polymerase chain reaction</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Predictive Value of Tests</topic><topic>Reproducibility of Results</topic><topic>Salmonella typhi - genetics</topic><topic>Salmonella typhi - immunology</topic><topic>Salmonella typhi - isolation & purification</topic><topic>Salmonella typhosa</topic><topic>Sensitivity and Specificity</topic><topic>Typhoid fever</topic><topic>Typhoid Fever - blood</topic><topic>Typhoid Fever - diagnosis</topic><topic>Typhoid Fever - drug therapy</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Munir, Tehmina</creatorcontrib><creatorcontrib>Lodhi, Munir</creatorcontrib><creatorcontrib>Ali, Salman</creatorcontrib><creatorcontrib>Hussain Zaidi, Syed Badshah</creatorcontrib><creatorcontrib>Razak, Suhail</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of the College of Physicians and Surgeons--Pakistan</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Munir, Tehmina</au><au>Lodhi, Munir</au><au>Ali, Salman</au><au>Hussain Zaidi, Syed Badshah</au><au>Razak, Suhail</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Early Diagnosis of Typhoid By PCR For FliC-d Gene of Salmonella Typhi in Patients Taking Antibiotics</atitle><jtitle>Journal of the College of Physicians and Surgeons--Pakistan</jtitle><addtitle>J Coll Physicians Surg Pak</addtitle><date>2015-09-01</date><risdate>2015</risdate><volume>25</volume><issue>9</issue><spage>662</spage><epage>666</epage><pages>662-666</pages><issn>1022-386X</issn><eissn>1681-7168</eissn><abstract>To compare PCR (Polymerase Chain Reaction) with blood culture, typhi-dot and Widal test for the diagnosis of typhoid in patients taking antibiotics.
Cross-sectional, comparative study.
National University of Sciences and Technology, Islamabad, Pakistan, from April 2013 to August 2014.
One hundred and five patients were included in the study. Blood was collected and inoculated into tryptone soya broth for culture. Any growth obtained was identified by API 20 E and confirmed by Salmonella anti-sera. Typhi-dot and Widal test were also done on all the samples. DNA extraction was done and PCR was carried out.
Among the 105 patients, 79 (75.2%) were males and 26 (24.8%) were females, with mean age of 20.64 ±14 years. Typhi-dot was positive in 58 (55.2%) and negative in 47 (44.8%) patients. Blood widal test was positive in 27 (25.7%) and negative in 78 (74.3%) patients. Salmonella Typhi was positive on blood culture in only one (1%) patient. PCR for Salmonella Typhi was positive in 102 (97.1%) and negative in 3 (2.9%) patients. Positive cases detected by PCR were significantly higher as compared to Typhi-dot (p < 0.001), blood Widal test (p < 0.001) and blood culture (p < 0.001).
Positivity rate of PCR was significantly higher as compared to blood culture, Typhi-dot or Widal test for diagnosing typhoid in patients who were already taking antibiotics.</abstract><cop>Pakistan</cop><pub>College of Physicians and Surgeons Pakistan</pub><pmid>26374362</pmid><tpages>5</tpages></addata></record> |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Adolescent Adult Agglutination Tests Anti-Bacterial Agents - administration & dosage Antibiotics Antibodies, Bacterial - blood Bacterial Typing Techniques Child Child, Preschool Cross-Sectional Studies Early Diagnosis Female Humans Immunoblotting Immunoenzyme Techniques Immunoglobulin M - blood Male Pakistan Polymerase chain reaction Polymerase Chain Reaction - methods Predictive Value of Tests Reproducibility of Results Salmonella typhi - genetics Salmonella typhi - immunology Salmonella typhi - isolation & purification Salmonella typhosa Sensitivity and Specificity Typhoid fever Typhoid Fever - blood Typhoid Fever - diagnosis Typhoid Fever - drug therapy Young Adult |
| title | Early Diagnosis of Typhoid By PCR For FliC-d Gene of Salmonella Typhi in Patients Taking Antibiotics |
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