Immunodiagnostic Properties of Wucheraria bancrofti SXP-1, a Potential Filarial Diagnostic Candidate Expressed in Tobacco Plant, Nicotiana tabacum
Transgenic tobacco plants were developed expressing WbSXP-1, a diagnostic antigen isolated from the cDNA library of L3 stage larvae of Wucheraria bancrofti. This antigen produced by recombinant Escherichia coli has been demonstrated by to be successful as potential diagnostic candidate against lymph...
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| Veröffentlicht in: | Applied biochemistry and biotechnology 2015-08, Vol.176 (7), p.1889-1903 |
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| container_title | Applied biochemistry and biotechnology |
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| creator | Ganapathy, Mathangi Chakravarthi, M Charles, S. Jason Harunipriya, P Jaiganesh, S Subramonian, N Kaliraj, P |
| description | Transgenic tobacco plants were developed expressing WbSXP-1, a diagnostic antigen isolated from the cDNA library of L3 stage larvae of Wucheraria bancrofti. This antigen produced by recombinant Escherichia coli has been demonstrated by to be successful as potential diagnostic candidate against lymphatic filariasis. A rapid format simple and qualitative flow through immune-filtration diagnostic kit has been developed for the identification of IgG antibodies to the recombinant WbSXP-1 and is being marketed by M/S Span Diagnostics Ltd in India and Africa. Here, we present the results of experiments on the transformation and expression of the same filarial antigen, WbSXP-1, in tobacco plant, Nicotiana tabacum, to produce plant-based diagnostic antigen. It was possible to successfully transform the tobacco plant with WbSXP-1, the integration of the parasite-specific gene in plants was confirmed by PCR amplification and the expression of the filarial protein by Western blotting. The immunoreactivity of the plant-produced WbSXP-1 was assessed based on its reaction with the monoclonal antibodies developed against the E. coli-produced protein. Immunological screening using clinical sera from patients indicates that the plant-produced protein is comparable to E. coli-produced diagnostic antigen. The result demonstrated that plants can be used as suitable expression systems for the production of diagnostic proteins against lymphatic filariasis, a neglected tropical infectious disease which has a negative impact on socioeconomic development. This is the first report of the integration, expression and efficacy of a diagnostic candidate of lymphatic filariasis in plants.Key MessageTransgenic tobacco plants with WbSXP-1, a filarial diagnostic candidate, were developed. The plant-produced protein showed immunoreactivity on par with the E. coli product. |
| doi_str_mv | 10.1007/s12010-015-1685-0 |
| format | Article |
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Jason ; Harunipriya, P ; Jaiganesh, S ; Subramonian, N ; Kaliraj, P</creator><creatorcontrib>Ganapathy, Mathangi ; Chakravarthi, M ; Charles, S. Jason ; Harunipriya, P ; Jaiganesh, S ; Subramonian, N ; Kaliraj, P</creatorcontrib><description>Transgenic tobacco plants were developed expressing WbSXP-1, a diagnostic antigen isolated from the cDNA library of L3 stage larvae of Wucheraria bancrofti. This antigen produced by recombinant Escherichia coli has been demonstrated by to be successful as potential diagnostic candidate against lymphatic filariasis. A rapid format simple and qualitative flow through immune-filtration diagnostic kit has been developed for the identification of IgG antibodies to the recombinant WbSXP-1 and is being marketed by M/S Span Diagnostics Ltd in India and Africa. Here, we present the results of experiments on the transformation and expression of the same filarial antigen, WbSXP-1, in tobacco plant, Nicotiana tabacum, to produce plant-based diagnostic antigen. It was possible to successfully transform the tobacco plant with WbSXP-1, the integration of the parasite-specific gene in plants was confirmed by PCR amplification and the expression of the filarial protein by Western blotting. The immunoreactivity of the plant-produced WbSXP-1 was assessed based on its reaction with the monoclonal antibodies developed against the E. coli-produced protein. Immunological screening using clinical sera from patients indicates that the plant-produced protein is comparable to E. coli-produced diagnostic antigen. The result demonstrated that plants can be used as suitable expression systems for the production of diagnostic proteins against lymphatic filariasis, a neglected tropical infectious disease which has a negative impact on socioeconomic development. This is the first report of the integration, expression and efficacy of a diagnostic candidate of lymphatic filariasis in plants.Key MessageTransgenic tobacco plants with WbSXP-1, a filarial diagnostic candidate, were developed. The plant-produced protein showed immunoreactivity on par with the E. coli product.</description><identifier>ISSN: 0273-2289</identifier><identifier>EISSN: 1559-0291</identifier><identifier>DOI: 10.1007/s12010-015-1685-0</identifier><identifier>PMID: 26043851</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Animals ; antigens ; Bacteria ; Biochemistry ; Biotechnology ; cDNA libraries ; Chemistry ; Chemistry and Materials Science ; Cloning, Molecular ; complementary DNA ; diagnostic techniques ; Diagnostics ; E coli ; Elephantiasis, Filarial - diagnosis ; Elephantiasis, Filarial - immunology ; Enzyme-Linked Immunosorbent Assay ; Escherichia coli ; Female ; filariasis ; Flowers & plants ; Gene Expression ; genes ; Genetic Engineering - methods ; Helminth Proteins - genetics ; Humans ; immunoglobulin G ; Immunology ; Infectious diseases ; Larvae ; Mice ; monoclonal antibodies ; Nicotiana - genetics ; Nicotiana tabacum ; Parasites ; patients ; Plants, Genetically Modified ; Polymerase Chain Reaction ; production technology ; screening ; socioeconomic development ; Tobacco ; Transformation, Genetic ; transgenic plants ; Vector-borne diseases ; Western blotting ; Wuchereria - genetics</subject><ispartof>Applied biochemistry and biotechnology, 2015-08, Vol.176 (7), p.1889-1903</ispartof><rights>Springer Science+Business Media New York 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-ce210df938f176b0853cdf0bad2b5b59daa4d28221f3cf1716f938be2a08ebb3</citedby><cites>FETCH-LOGICAL-c499t-ce210df938f176b0853cdf0bad2b5b59daa4d28221f3cf1716f938be2a08ebb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12010-015-1685-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12010-015-1685-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26043851$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ganapathy, Mathangi</creatorcontrib><creatorcontrib>Chakravarthi, M</creatorcontrib><creatorcontrib>Charles, S. Jason</creatorcontrib><creatorcontrib>Harunipriya, P</creatorcontrib><creatorcontrib>Jaiganesh, S</creatorcontrib><creatorcontrib>Subramonian, N</creatorcontrib><creatorcontrib>Kaliraj, P</creatorcontrib><title>Immunodiagnostic Properties of Wucheraria bancrofti SXP-1, a Potential Filarial Diagnostic Candidate Expressed in Tobacco Plant, Nicotiana tabacum</title><title>Applied biochemistry and biotechnology</title><addtitle>Appl Biochem Biotechnol</addtitle><addtitle>Appl Biochem Biotechnol</addtitle><description>Transgenic tobacco plants were developed expressing WbSXP-1, a diagnostic antigen isolated from the cDNA library of L3 stage larvae of Wucheraria bancrofti. This antigen produced by recombinant Escherichia coli has been demonstrated by to be successful as potential diagnostic candidate against lymphatic filariasis. A rapid format simple and qualitative flow through immune-filtration diagnostic kit has been developed for the identification of IgG antibodies to the recombinant WbSXP-1 and is being marketed by M/S Span Diagnostics Ltd in India and Africa. Here, we present the results of experiments on the transformation and expression of the same filarial antigen, WbSXP-1, in tobacco plant, Nicotiana tabacum, to produce plant-based diagnostic antigen. It was possible to successfully transform the tobacco plant with WbSXP-1, the integration of the parasite-specific gene in plants was confirmed by PCR amplification and the expression of the filarial protein by Western blotting. The immunoreactivity of the plant-produced WbSXP-1 was assessed based on its reaction with the monoclonal antibodies developed against the E. coli-produced protein. Immunological screening using clinical sera from patients indicates that the plant-produced protein is comparable to E. coli-produced diagnostic antigen. The result demonstrated that plants can be used as suitable expression systems for the production of diagnostic proteins against lymphatic filariasis, a neglected tropical infectious disease which has a negative impact on socioeconomic development. This is the first report of the integration, expression and efficacy of a diagnostic candidate of lymphatic filariasis in plants.Key MessageTransgenic tobacco plants with WbSXP-1, a filarial diagnostic candidate, were developed. The plant-produced protein showed immunoreactivity on par with the E. coli product.</description><subject>Animals</subject><subject>antigens</subject><subject>Bacteria</subject><subject>Biochemistry</subject><subject>Biotechnology</subject><subject>cDNA libraries</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Cloning, Molecular</subject><subject>complementary DNA</subject><subject>diagnostic techniques</subject><subject>Diagnostics</subject><subject>E coli</subject><subject>Elephantiasis, Filarial - diagnosis</subject><subject>Elephantiasis, Filarial - immunology</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Escherichia coli</subject><subject>Female</subject><subject>filariasis</subject><subject>Flowers & plants</subject><subject>Gene Expression</subject><subject>genes</subject><subject>Genetic Engineering - methods</subject><subject>Helminth Proteins - genetics</subject><subject>Humans</subject><subject>immunoglobulin G</subject><subject>Immunology</subject><subject>Infectious diseases</subject><subject>Larvae</subject><subject>Mice</subject><subject>monoclonal antibodies</subject><subject>Nicotiana - genetics</subject><subject>Nicotiana tabacum</subject><subject>Parasites</subject><subject>patients</subject><subject>Plants, Genetically Modified</subject><subject>Polymerase Chain Reaction</subject><subject>production technology</subject><subject>screening</subject><subject>socioeconomic development</subject><subject>Tobacco</subject><subject>Transformation, Genetic</subject><subject>transgenic plants</subject><subject>Vector-borne diseases</subject><subject>Western blotting</subject><subject>Wuchereria - genetics</subject><issn>0273-2289</issn><issn>1559-0291</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkc9u1DAQxi0EokvhAbiAJS4cGhg7ceIc0dJCpQpW6iK4WWPHWVwl9mI7ErwGT4xXW_6IA2IuPszv-2Y8HyGPGbxgAN3LxDgwqICJirVSVHCHrJgQfQW8Z3fJCnhXV5zL_oQ8SOkGgHEpuvvkhLfQ1FKwFfl-Oc-LD4PDnQ8pO0M3MextzM4mGkb6cTGfbcTokGr0JoYxO3r9aVOxM4p0E7L12eFEL9x0gCb6-rfTGv3gBsyWnn_dR5uSHajzdBs0GhPoZkKfz-g7Z0Kx8EgzlsYyPyT3RpySfXT7npLtxfl2_ba6ev_mcv3qqjJN3-fKWM5gGPtajqxrNUhRm2EEjQPXQot-QGwGLjlnY20KwtoDqy1HkFbr-pQ8P9ruY_iy2JTV7JKxU9nKhiWpouBd1zTQ_QdazilY04mCPvsLvQlL9OUfhSoY7yXwQrEjVQ6aUrSj2kc3Y_ymGKhDtOoYrSrRqkO0Cormya3zomc7_FL8zLIA_Aik0vI7G_8Y_Q_Xp0fRiEHhLrqkPlwXqIVSdS15_QP6h7hg</recordid><startdate>20150801</startdate><enddate>20150801</enddate><creator>Ganapathy, Mathangi</creator><creator>Chakravarthi, M</creator><creator>Charles, S. 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Jason</creatorcontrib><creatorcontrib>Harunipriya, P</creatorcontrib><creatorcontrib>Jaiganesh, S</creatorcontrib><creatorcontrib>Subramonian, N</creatorcontrib><creatorcontrib>Kaliraj, P</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Applied biochemistry and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ganapathy, Mathangi</au><au>Chakravarthi, M</au><au>Charles, S. Jason</au><au>Harunipriya, P</au><au>Jaiganesh, S</au><au>Subramonian, N</au><au>Kaliraj, P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunodiagnostic Properties of Wucheraria bancrofti SXP-1, a Potential Filarial Diagnostic Candidate Expressed in Tobacco Plant, Nicotiana tabacum</atitle><jtitle>Applied biochemistry and biotechnology</jtitle><stitle>Appl Biochem Biotechnol</stitle><addtitle>Appl Biochem Biotechnol</addtitle><date>2015-08-01</date><risdate>2015</risdate><volume>176</volume><issue>7</issue><spage>1889</spage><epage>1903</epage><pages>1889-1903</pages><issn>0273-2289</issn><eissn>1559-0291</eissn><abstract>Transgenic tobacco plants were developed expressing WbSXP-1, a diagnostic antigen isolated from the cDNA library of L3 stage larvae of Wucheraria bancrofti. This antigen produced by recombinant Escherichia coli has been demonstrated by to be successful as potential diagnostic candidate against lymphatic filariasis. A rapid format simple and qualitative flow through immune-filtration diagnostic kit has been developed for the identification of IgG antibodies to the recombinant WbSXP-1 and is being marketed by M/S Span Diagnostics Ltd in India and Africa. Here, we present the results of experiments on the transformation and expression of the same filarial antigen, WbSXP-1, in tobacco plant, Nicotiana tabacum, to produce plant-based diagnostic antigen. It was possible to successfully transform the tobacco plant with WbSXP-1, the integration of the parasite-specific gene in plants was confirmed by PCR amplification and the expression of the filarial protein by Western blotting. The immunoreactivity of the plant-produced WbSXP-1 was assessed based on its reaction with the monoclonal antibodies developed against the E. coli-produced protein. Immunological screening using clinical sera from patients indicates that the plant-produced protein is comparable to E. coli-produced diagnostic antigen. The result demonstrated that plants can be used as suitable expression systems for the production of diagnostic proteins against lymphatic filariasis, a neglected tropical infectious disease which has a negative impact on socioeconomic development. This is the first report of the integration, expression and efficacy of a diagnostic candidate of lymphatic filariasis in plants.Key MessageTransgenic tobacco plants with WbSXP-1, a filarial diagnostic candidate, were developed. The plant-produced protein showed immunoreactivity on par with the E. coli product.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>26043851</pmid><doi>10.1007/s12010-015-1685-0</doi><tpages>15</tpages></addata></record> |
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| subjects | Animals antigens Bacteria Biochemistry Biotechnology cDNA libraries Chemistry Chemistry and Materials Science Cloning, Molecular complementary DNA diagnostic techniques Diagnostics E coli Elephantiasis, Filarial - diagnosis Elephantiasis, Filarial - immunology Enzyme-Linked Immunosorbent Assay Escherichia coli Female filariasis Flowers & plants Gene Expression genes Genetic Engineering - methods Helminth Proteins - genetics Humans immunoglobulin G Immunology Infectious diseases Larvae Mice monoclonal antibodies Nicotiana - genetics Nicotiana tabacum Parasites patients Plants, Genetically Modified Polymerase Chain Reaction production technology screening socioeconomic development Tobacco Transformation, Genetic transgenic plants Vector-borne diseases Western blotting Wuchereria - genetics |
| title | Immunodiagnostic Properties of Wucheraria bancrofti SXP-1, a Potential Filarial Diagnostic Candidate Expressed in Tobacco Plant, Nicotiana tabacum |
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