Phylogenetic Diversity of Sponge-Associated Fungi from the Caribbean and the Pacific of Panama and Their In Vitro Effect on Angiotensin and Endothelin Receptors
Fungi occupy an important ecological niche in the marine environment, and marine fungi possess an immense biotechnological potential. This study documents the fungal diversity associated with 39 species of sponges and determines their potential to produce secondary metabolites capable of interacting...
Gespeichert in:
| Veröffentlicht in: | Marine biotechnology (New York, N.Y.) N.Y.), 2015-10, Vol.17 (5), p.533-564 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 564 |
|---|---|
| container_issue | 5 |
| container_start_page | 533 |
| container_title | Marine biotechnology (New York, N.Y.) |
| container_volume | 17 |
| creator | Bolaños, Jessica De León, Luis Fernando Ochoa, Edgardo Darias, José Raja, Huzefa A. Shearer, Carol A. Miller, Andrew N. Vanderheyden, Patrick Porras-Alfaro, Andrea Caballero-George, Catherina |
| description | Fungi occupy an important ecological niche in the marine environment, and marine fungi possess an immense biotechnological potential. This study documents the fungal diversity associated with 39 species of sponges and determines their potential to produce secondary metabolites capable of interacting with mammalian G-protein-coupled receptors involved in blood pressure regulation. Total genomic DNA was extracted from 563 representative fungal strains obtained from marine sponges collected by SCUBA from the Caribbean and the Pacific regions of Panama. A total of 194 operational taxonomic units were found with 58 % represented by singletons based on the internal transcribed spacer (ITS) and partial large subunit (LSU) rDNA regions. Marine sponges were highly dominated by Ascomycota fungi (95.6 %) and represented by two major classes, Sordariomycetes and Dothideomycetes. Rarefaction curves showed no saturation, indicating that further efforts are needed to reveal the entire diversity at this site. Several unique clades were found during phylogenetic analysis with the highest diversity of unique clades in the order Pleosporales. From the 65 cultures tested to determine their in vitro effect on angiotensin and endothelin receptors, the extracts of Fusarium sp. and Phoma sp. blocked the activation of these receptors by more than 50 % of the control and seven others inhibited between 30 and 45 %. Our results indicate that marine sponges from Panama are a “hot spot” of fungal diversity as well as a rich resource for capturing, cataloguing, and assessing the pharmacological potential of substances present in previously undiscovered fungi associated with marine sponges. |
| doi_str_mv | 10.1007/s10126-015-9634-z |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1712774322</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1712774322</sourcerecordid><originalsourceid>FETCH-LOGICAL-c499t-af1d47fede0f21d01330f7f102ff5983c8a90ac5e7498839aa9af7babdbf17233</originalsourceid><addsrcrecordid>eNqFkc1u1DAYRSMEoj_wAGzAEhs2Af9k4ng5mk6hUiVGtGVrfUk-z7jK2IPtIE2fhketpykVYgErx_a5x3ZuUbxh9COjVH6KjDJel5TNSlWLqrx7VhyzStQl56J-_vTNm6PiJMZbmjNS0JfFEa8pr1XVHBe_Vpv94NfoMNmOnNmfGKJNe-INudp5t8ZyHqPvLCTsyfno1paY4LckbZAsINi2RXAEXP-wsoLOmuzJ6RU42MLDzvUGbSAXjny3KXiyNAa7RLwj86zzCV20k2Lpep81Q55-ww53yYf4qnhhYIj4-nE8LW7Ol9eLL-Xl188Xi_ll2VVKpRIM6ytpsEdqOOspE4IaaRjlxsxUI7oGFIVuhrJSTSMUgAIjW2j71jDJhTgtPkzeXfA_RoxJb23scBjAoR-jZpJxKSuRf-3_UTqTXDV1k9H3f6G3fgwuP-RAVUrwXF6m2ER1wccY0OhdsFsIe82oPjStp6Z1hvWhaX2XM28fzWO7xf4p8bvaDPAJiHkrFxn-OPof1ndTyIDXsA426purfMeaUlbVopHiHot7vls</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1704932015</pqid></control><display><type>article</type><title>Phylogenetic Diversity of Sponge-Associated Fungi from the Caribbean and the Pacific of Panama and Their In Vitro Effect on Angiotensin and Endothelin Receptors</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Bolaños, Jessica ; De León, Luis Fernando ; Ochoa, Edgardo ; Darias, José ; Raja, Huzefa A. ; Shearer, Carol A. ; Miller, Andrew N. ; Vanderheyden, Patrick ; Porras-Alfaro, Andrea ; Caballero-George, Catherina</creator><creatorcontrib>Bolaños, Jessica ; De León, Luis Fernando ; Ochoa, Edgardo ; Darias, José ; Raja, Huzefa A. ; Shearer, Carol A. ; Miller, Andrew N. ; Vanderheyden, Patrick ; Porras-Alfaro, Andrea ; Caballero-George, Catherina</creatorcontrib><description>Fungi occupy an important ecological niche in the marine environment, and marine fungi possess an immense biotechnological potential. This study documents the fungal diversity associated with 39 species of sponges and determines their potential to produce secondary metabolites capable of interacting with mammalian G-protein-coupled receptors involved in blood pressure regulation. Total genomic DNA was extracted from 563 representative fungal strains obtained from marine sponges collected by SCUBA from the Caribbean and the Pacific regions of Panama. A total of 194 operational taxonomic units were found with 58 % represented by singletons based on the internal transcribed spacer (ITS) and partial large subunit (LSU) rDNA regions. Marine sponges were highly dominated by Ascomycota fungi (95.6 %) and represented by two major classes, Sordariomycetes and Dothideomycetes. Rarefaction curves showed no saturation, indicating that further efforts are needed to reveal the entire diversity at this site. Several unique clades were found during phylogenetic analysis with the highest diversity of unique clades in the order Pleosporales. From the 65 cultures tested to determine their in vitro effect on angiotensin and endothelin receptors, the extracts of Fusarium sp. and Phoma sp. blocked the activation of these receptors by more than 50 % of the control and seven others inhibited between 30 and 45 %. Our results indicate that marine sponges from Panama are a “hot spot” of fungal diversity as well as a rich resource for capturing, cataloguing, and assessing the pharmacological potential of substances present in previously undiscovered fungi associated with marine sponges.</description><identifier>ISSN: 1436-2228</identifier><identifier>EISSN: 1436-2236</identifier><identifier>DOI: 10.1007/s10126-015-9634-z</identifier><identifier>PMID: 26026948</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Analysis ; Angiotensins ; Animals ; Aquatic ecosystems ; Ascomycota ; Ascomycota - classification ; Ascomycota - genetics ; Biodiversity ; Biomedical and Life Sciences ; Biotechnology ; Blood pressure ; Caribbean Region ; DNA, Ribosomal - genetics ; DNA, Ribosomal Spacer - genetics ; Dothideomycetes ; Engineering ; Freshwater & Marine Ecology ; Fungi ; Fungi - classification ; Fungi - genetics ; Fusarium ; Invertebrates ; Islands ; Life Sciences ; Marine ecology ; Marine environment ; Metabolites ; Microbiology ; Microorganisms ; National parks ; Original Article ; Panama ; Pharmacology ; Phoma ; Phylogenetics ; Phylogeny ; Pleosporales ; Porifera - microbiology ; Receptors, Endothelin - metabolism ; Secondary metabolites ; Smooth muscle ; Sordariomycetes ; Species diversity ; Studies ; Taxonomy ; Zoology</subject><ispartof>Marine biotechnology (New York, N.Y.), 2015-10, Vol.17 (5), p.533-564</ispartof><rights>Springer Science+Business Media New York 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-af1d47fede0f21d01330f7f102ff5983c8a90ac5e7498839aa9af7babdbf17233</citedby><cites>FETCH-LOGICAL-c499t-af1d47fede0f21d01330f7f102ff5983c8a90ac5e7498839aa9af7babdbf17233</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10126-015-9634-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10126-015-9634-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26026948$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bolaños, Jessica</creatorcontrib><creatorcontrib>De León, Luis Fernando</creatorcontrib><creatorcontrib>Ochoa, Edgardo</creatorcontrib><creatorcontrib>Darias, José</creatorcontrib><creatorcontrib>Raja, Huzefa A.</creatorcontrib><creatorcontrib>Shearer, Carol A.</creatorcontrib><creatorcontrib>Miller, Andrew N.</creatorcontrib><creatorcontrib>Vanderheyden, Patrick</creatorcontrib><creatorcontrib>Porras-Alfaro, Andrea</creatorcontrib><creatorcontrib>Caballero-George, Catherina</creatorcontrib><title>Phylogenetic Diversity of Sponge-Associated Fungi from the Caribbean and the Pacific of Panama and Their In Vitro Effect on Angiotensin and Endothelin Receptors</title><title>Marine biotechnology (New York, N.Y.)</title><addtitle>Mar Biotechnol</addtitle><addtitle>Mar Biotechnol (NY)</addtitle><description>Fungi occupy an important ecological niche in the marine environment, and marine fungi possess an immense biotechnological potential. This study documents the fungal diversity associated with 39 species of sponges and determines their potential to produce secondary metabolites capable of interacting with mammalian G-protein-coupled receptors involved in blood pressure regulation. Total genomic DNA was extracted from 563 representative fungal strains obtained from marine sponges collected by SCUBA from the Caribbean and the Pacific regions of Panama. A total of 194 operational taxonomic units were found with 58 % represented by singletons based on the internal transcribed spacer (ITS) and partial large subunit (LSU) rDNA regions. Marine sponges were highly dominated by Ascomycota fungi (95.6 %) and represented by two major classes, Sordariomycetes and Dothideomycetes. Rarefaction curves showed no saturation, indicating that further efforts are needed to reveal the entire diversity at this site. Several unique clades were found during phylogenetic analysis with the highest diversity of unique clades in the order Pleosporales. From the 65 cultures tested to determine their in vitro effect on angiotensin and endothelin receptors, the extracts of Fusarium sp. and Phoma sp. blocked the activation of these receptors by more than 50 % of the control and seven others inhibited between 30 and 45 %. Our results indicate that marine sponges from Panama are a “hot spot” of fungal diversity as well as a rich resource for capturing, cataloguing, and assessing the pharmacological potential of substances present in previously undiscovered fungi associated with marine sponges.</description><subject>Analysis</subject><subject>Angiotensins</subject><subject>Animals</subject><subject>Aquatic ecosystems</subject><subject>Ascomycota</subject><subject>Ascomycota - classification</subject><subject>Ascomycota - genetics</subject><subject>Biodiversity</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Blood pressure</subject><subject>Caribbean Region</subject><subject>DNA, Ribosomal - genetics</subject><subject>DNA, Ribosomal Spacer - genetics</subject><subject>Dothideomycetes</subject><subject>Engineering</subject><subject>Freshwater & Marine Ecology</subject><subject>Fungi</subject><subject>Fungi - classification</subject><subject>Fungi - genetics</subject><subject>Fusarium</subject><subject>Invertebrates</subject><subject>Islands</subject><subject>Life Sciences</subject><subject>Marine ecology</subject><subject>Marine environment</subject><subject>Metabolites</subject><subject>Microbiology</subject><subject>Microorganisms</subject><subject>National parks</subject><subject>Original Article</subject><subject>Panama</subject><subject>Pharmacology</subject><subject>Phoma</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Pleosporales</subject><subject>Porifera - microbiology</subject><subject>Receptors, Endothelin - metabolism</subject><subject>Secondary metabolites</subject><subject>Smooth muscle</subject><subject>Sordariomycetes</subject><subject>Species diversity</subject><subject>Studies</subject><subject>Taxonomy</subject><subject>Zoology</subject><issn>1436-2228</issn><issn>1436-2236</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkc1u1DAYRSMEoj_wAGzAEhs2Af9k4ng5mk6hUiVGtGVrfUk-z7jK2IPtIE2fhketpykVYgErx_a5x3ZuUbxh9COjVH6KjDJel5TNSlWLqrx7VhyzStQl56J-_vTNm6PiJMZbmjNS0JfFEa8pr1XVHBe_Vpv94NfoMNmOnNmfGKJNe-INudp5t8ZyHqPvLCTsyfno1paY4LckbZAsINi2RXAEXP-wsoLOmuzJ6RU42MLDzvUGbSAXjny3KXiyNAa7RLwj86zzCV20k2Lpep81Q55-ww53yYf4qnhhYIj4-nE8LW7Ol9eLL-Xl188Xi_ll2VVKpRIM6ytpsEdqOOspE4IaaRjlxsxUI7oGFIVuhrJSTSMUgAIjW2j71jDJhTgtPkzeXfA_RoxJb23scBjAoR-jZpJxKSuRf-3_UTqTXDV1k9H3f6G3fgwuP-RAVUrwXF6m2ER1wccY0OhdsFsIe82oPjStp6Z1hvWhaX2XM28fzWO7xf4p8bvaDPAJiHkrFxn-OPof1ndTyIDXsA426purfMeaUlbVopHiHot7vls</recordid><startdate>20151001</startdate><enddate>20151001</enddate><creator>Bolaños, Jessica</creator><creator>De León, Luis Fernando</creator><creator>Ochoa, Edgardo</creator><creator>Darias, José</creator><creator>Raja, Huzefa A.</creator><creator>Shearer, Carol A.</creator><creator>Miller, Andrew N.</creator><creator>Vanderheyden, Patrick</creator><creator>Porras-Alfaro, Andrea</creator><creator>Caballero-George, Catherina</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QO</scope><scope>7TN</scope><scope>7U9</scope><scope>7WY</scope><scope>7WZ</scope><scope>7X7</scope><scope>7XB</scope><scope>87Z</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8FL</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BEZIV</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FR3</scope><scope>FRNLG</scope><scope>FYUFA</scope><scope>F~G</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>H95</scope><scope>H98</scope><scope>H99</scope><scope>HCIFZ</scope><scope>K60</scope><scope>K6~</scope><scope>K9.</scope><scope>L.-</scope><scope>L.F</scope><scope>L.G</scope><scope>L6V</scope><scope>LK8</scope><scope>M0C</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>P64</scope><scope>PCBAR</scope><scope>PQBIZ</scope><scope>PQBZA</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20151001</creationdate><title>Phylogenetic Diversity of Sponge-Associated Fungi from the Caribbean and the Pacific of Panama and Their In Vitro Effect on Angiotensin and Endothelin Receptors</title><author>Bolaños, Jessica ; De León, Luis Fernando ; Ochoa, Edgardo ; Darias, José ; Raja, Huzefa A. ; Shearer, Carol A. ; Miller, Andrew N. ; Vanderheyden, Patrick ; Porras-Alfaro, Andrea ; Caballero-George, Catherina</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c499t-af1d47fede0f21d01330f7f102ff5983c8a90ac5e7498839aa9af7babdbf17233</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Analysis</topic><topic>Angiotensins</topic><topic>Animals</topic><topic>Aquatic ecosystems</topic><topic>Ascomycota</topic><topic>Ascomycota - classification</topic><topic>Ascomycota - genetics</topic><topic>Biodiversity</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Blood pressure</topic><topic>Caribbean Region</topic><topic>DNA, Ribosomal - genetics</topic><topic>DNA, Ribosomal Spacer - genetics</topic><topic>Dothideomycetes</topic><topic>Engineering</topic><topic>Freshwater & Marine Ecology</topic><topic>Fungi</topic><topic>Fungi - classification</topic><topic>Fungi - genetics</topic><topic>Fusarium</topic><topic>Invertebrates</topic><topic>Islands</topic><topic>Life Sciences</topic><topic>Marine ecology</topic><topic>Marine environment</topic><topic>Metabolites</topic><topic>Microbiology</topic><topic>Microorganisms</topic><topic>National parks</topic><topic>Original Article</topic><topic>Panama</topic><topic>Pharmacology</topic><topic>Phoma</topic><topic>Phylogenetics</topic><topic>Phylogeny</topic><topic>Pleosporales</topic><topic>Porifera - microbiology</topic><topic>Receptors, Endothelin - metabolism</topic><topic>Secondary metabolites</topic><topic>Smooth muscle</topic><topic>Sordariomycetes</topic><topic>Species diversity</topic><topic>Studies</topic><topic>Taxonomy</topic><topic>Zoology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bolaños, Jessica</creatorcontrib><creatorcontrib>De León, Luis Fernando</creatorcontrib><creatorcontrib>Ochoa, Edgardo</creatorcontrib><creatorcontrib>Darias, José</creatorcontrib><creatorcontrib>Raja, Huzefa A.</creatorcontrib><creatorcontrib>Shearer, Carol A.</creatorcontrib><creatorcontrib>Miller, Andrew N.</creatorcontrib><creatorcontrib>Vanderheyden, Patrick</creatorcontrib><creatorcontrib>Porras-Alfaro, Andrea</creatorcontrib><creatorcontrib>Caballero-George, Catherina</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Oceanic Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>ABI/INFORM Collection</collection><collection>ABI/INFORM Global (PDF only)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ABI/INFORM Global (Alumni Edition)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ABI/INFORM Collection (Alumni Edition)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Business Premium Collection</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Business Premium Collection (Alumni)</collection><collection>Health Research Premium Collection</collection><collection>ABI/INFORM Global (Corporate)</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Business Collection (Alumni Edition)</collection><collection>ProQuest Business Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ABI/INFORM Professional Advanced</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>ABI/INFORM Global</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>One Business (ProQuest)</collection><collection>ProQuest One Business (Alumni)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Marine biotechnology (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bolaños, Jessica</au><au>De León, Luis Fernando</au><au>Ochoa, Edgardo</au><au>Darias, José</au><au>Raja, Huzefa A.</au><au>Shearer, Carol A.</au><au>Miller, Andrew N.</au><au>Vanderheyden, Patrick</au><au>Porras-Alfaro, Andrea</au><au>Caballero-George, Catherina</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phylogenetic Diversity of Sponge-Associated Fungi from the Caribbean and the Pacific of Panama and Their In Vitro Effect on Angiotensin and Endothelin Receptors</atitle><jtitle>Marine biotechnology (New York, N.Y.)</jtitle><stitle>Mar Biotechnol</stitle><addtitle>Mar Biotechnol (NY)</addtitle><date>2015-10-01</date><risdate>2015</risdate><volume>17</volume><issue>5</issue><spage>533</spage><epage>564</epage><pages>533-564</pages><issn>1436-2228</issn><eissn>1436-2236</eissn><abstract>Fungi occupy an important ecological niche in the marine environment, and marine fungi possess an immense biotechnological potential. This study documents the fungal diversity associated with 39 species of sponges and determines their potential to produce secondary metabolites capable of interacting with mammalian G-protein-coupled receptors involved in blood pressure regulation. Total genomic DNA was extracted from 563 representative fungal strains obtained from marine sponges collected by SCUBA from the Caribbean and the Pacific regions of Panama. A total of 194 operational taxonomic units were found with 58 % represented by singletons based on the internal transcribed spacer (ITS) and partial large subunit (LSU) rDNA regions. Marine sponges were highly dominated by Ascomycota fungi (95.6 %) and represented by two major classes, Sordariomycetes and Dothideomycetes. Rarefaction curves showed no saturation, indicating that further efforts are needed to reveal the entire diversity at this site. Several unique clades were found during phylogenetic analysis with the highest diversity of unique clades in the order Pleosporales. From the 65 cultures tested to determine their in vitro effect on angiotensin and endothelin receptors, the extracts of Fusarium sp. and Phoma sp. blocked the activation of these receptors by more than 50 % of the control and seven others inhibited between 30 and 45 %. Our results indicate that marine sponges from Panama are a “hot spot” of fungal diversity as well as a rich resource for capturing, cataloguing, and assessing the pharmacological potential of substances present in previously undiscovered fungi associated with marine sponges.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>26026948</pmid><doi>10.1007/s10126-015-9634-z</doi><tpages>32</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1436-2228 |
| ispartof | Marine biotechnology (New York, N.Y.), 2015-10, Vol.17 (5), p.533-564 |
| issn | 1436-2228 1436-2236 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1712774322 |
| source | MEDLINE; SpringerLink Journals |
| subjects | Analysis Angiotensins Animals Aquatic ecosystems Ascomycota Ascomycota - classification Ascomycota - genetics Biodiversity Biomedical and Life Sciences Biotechnology Blood pressure Caribbean Region DNA, Ribosomal - genetics DNA, Ribosomal Spacer - genetics Dothideomycetes Engineering Freshwater & Marine Ecology Fungi Fungi - classification Fungi - genetics Fusarium Invertebrates Islands Life Sciences Marine ecology Marine environment Metabolites Microbiology Microorganisms National parks Original Article Panama Pharmacology Phoma Phylogenetics Phylogeny Pleosporales Porifera - microbiology Receptors, Endothelin - metabolism Secondary metabolites Smooth muscle Sordariomycetes Species diversity Studies Taxonomy Zoology |
| title | Phylogenetic Diversity of Sponge-Associated Fungi from the Caribbean and the Pacific of Panama and Their In Vitro Effect on Angiotensin and Endothelin Receptors |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-11T13%3A25%3A21IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Phylogenetic%20Diversity%20of%20Sponge-Associated%20Fungi%20from%20the%20Caribbean%20and%20the%20Pacific%20of%20Panama%20and%20Their%20In%20Vitro%20Effect%20on%20Angiotensin%20and%20Endothelin%20Receptors&rft.jtitle=Marine%20biotechnology%20(New%20York,%20N.Y.)&rft.au=Bola%C3%B1os,%20Jessica&rft.date=2015-10-01&rft.volume=17&rft.issue=5&rft.spage=533&rft.epage=564&rft.pages=533-564&rft.issn=1436-2228&rft.eissn=1436-2236&rft_id=info:doi/10.1007/s10126-015-9634-z&rft_dat=%3Cproquest_cross%3E1712774322%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1704932015&rft_id=info:pmid/26026948&rfr_iscdi=true |