Green Synthesis of Silver Nano-particles by Macrococcus bovicus and Its Immobilization onto Montmorillonite Clay for Antimicrobial Functionality
Macrococcus bovicus was locally isolated from soil and used in the green synthesis of nano-scaling silver (NSAg). It was immobilized on a sodic-montmorillonite clay (MMT1) and cetyltrimethylammonium bromide-modified montmorillonite (MMT2) which was also calcined at 300 °C (MMT3). The NSAg clays were...
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| Veröffentlicht in: | Applied biochemistry and biotechnology 2015-08, Vol.176 (8), p.2225-2241 |
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| creator | Abdel-Aziz, Mohamed S. Abou-El-Sherbini, Khaled S. Hamzawy, Esmat M. A. Amr, Mohey H. A. El-Dafrawy, Shady |
| description | Macrococcus
bovicus
was locally isolated from soil and used in the green synthesis of nano-scaling silver (NSAg). It was immobilized on a sodic-montmorillonite clay (MMT1) and cetyltrimethylammonium bromide-modified montmorillonite (MMT2) which was also calcined at 300 °C (MMT3). The NSAg clays were characterized by X-ray fluorescence, Fourier transform infrared spectra, X-ray diffractometry, surface area measurement, UV–Vis spectrometry, scanning electron microscope, transmission electron microscope and thermogravimetric analysis. NSAg was confirmed to be included in the interparticular cavities of the clay sheets and its mechanical stability was evidenced. The antimicrobial activity of the NSAg-modified clays was investigated against
Staphylococcus aureus
,
Escherichia coli
and
Candida albicans
using the cup plate and the plate count techniques. The antimicrobial activity of the NSAg clays was confirmed and attributed to the caging of NSAg in MMT cavities. MMT3 was found to inhibit the microbial growth to as high as 65 % as observed from the plate count method.
Graphical Abstract
Scheme of the biosynthesis of nano-scaling Ag and its immobilization and antimicrobial application |
| doi_str_mv | 10.1007/s12010-015-1710-3 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1712773281</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3774578311</sourcerecordid><originalsourceid>FETCH-LOGICAL-c512t-308f32d97d23e537e01388360c9775f36bd4741592f86d1ef015e058f44caea3</originalsourceid><addsrcrecordid>eNqNkc9uFSEUxonR2Gv1AdwYEjduRvlzGZhlc9PWm7S6aPcThjkoDQNXYJqMT-Ejy820pjExccOB8Ps-Dt9B6C0lHykh8lOmjFDSECoaKuuGP0MbKkTXENbR52hDmOQNY6o7Qa9yviOEMiXkS3TC2qrnSm7Qr8sEEPDNEsp3yC7jaPGN8_eQ8BcdYnPQqTjjIeNhwdfapGiiMXM9xnt3rDqMeF8y3k9THJx3P3VxMeAYSsTXdZ1ict7H4ArgndcLtjHhs1Dc5KrZ4LTHF3MwR5H2riyv0QurfYY3D_UU3V6c3-4-N1dfL_e7s6vGCMpKw4mynI2dHBkHwSUQypXiLTGdlMLydhi3cktFx6xqRwq2hgREKLvdGg2an6IPq-0hxR8z5NJPLhvwXgeIc-5rnkxKzhT9D5TwtusEkRV9_xd6F-dUP7ZSlEulWKXoStUAck5g-0Nyk05LT0l_HGy_DravPR8bIT2vmncPzvMwwfhH8TjJCrAVyPUqfIP05Ol_uv4GDqCurA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1703137882</pqid></control><display><type>article</type><title>Green Synthesis of Silver Nano-particles by Macrococcus bovicus and Its Immobilization onto Montmorillonite Clay for Antimicrobial Functionality</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Abdel-Aziz, Mohamed S. ; Abou-El-Sherbini, Khaled S. ; Hamzawy, Esmat M. A. ; Amr, Mohey H. A. ; El-Dafrawy, Shady</creator><creatorcontrib>Abdel-Aziz, Mohamed S. ; Abou-El-Sherbini, Khaled S. ; Hamzawy, Esmat M. A. ; Amr, Mohey H. A. ; El-Dafrawy, Shady</creatorcontrib><description>Macrococcus
bovicus
was locally isolated from soil and used in the green synthesis of nano-scaling silver (NSAg). It was immobilized on a sodic-montmorillonite clay (MMT1) and cetyltrimethylammonium bromide-modified montmorillonite (MMT2) which was also calcined at 300 °C (MMT3). The NSAg clays were characterized by X-ray fluorescence, Fourier transform infrared spectra, X-ray diffractometry, surface area measurement, UV–Vis spectrometry, scanning electron microscope, transmission electron microscope and thermogravimetric analysis. NSAg was confirmed to be included in the interparticular cavities of the clay sheets and its mechanical stability was evidenced. The antimicrobial activity of the NSAg-modified clays was investigated against
Staphylococcus aureus
,
Escherichia coli
and
Candida albicans
using the cup plate and the plate count techniques. The antimicrobial activity of the NSAg clays was confirmed and attributed to the caging of NSAg in MMT cavities. MMT3 was found to inhibit the microbial growth to as high as 65 % as observed from the plate count method.
Graphical Abstract
Scheme of the biosynthesis of nano-scaling Ag and its immobilization and antimicrobial application</description><identifier>ISSN: 0273-2289</identifier><identifier>EISSN: 1559-0291</identifier><identifier>DOI: 10.1007/s12010-015-1710-3</identifier><identifier>PMID: 26100387</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Aluminum Silicates - chemistry ; Anti-Infective Agents - pharmacology ; Antimicrobial agents ; Bentonite - pharmacology ; Biochemistry ; Biosynthesis ; Biotechnology ; Candida albicans ; Chemistry ; Chemistry and Materials Science ; Clay ; E coli ; Escherichia coli ; Fourier transforms ; Green Chemistry Technology - methods ; Metal Nanoparticles - chemistry ; Metal Nanoparticles - ultrastructure ; Microbial Sensitivity Tests ; Montmorillonite ; Nanoparticles ; Silver ; Silver - chemistry ; Spectrometry ; Spectrophotometry, Ultraviolet ; Spectroscopy, Fourier Transform Infrared ; Staphylococcaceae - drug effects ; Staphylococcaceae - metabolism ; Staphylococcus aureus ; Staphylococcus aureus - drug effects ; Thermogravimetric analysis ; Thermogravimetry ; X-Ray Diffraction ; X-ray fluorescence</subject><ispartof>Applied biochemistry and biotechnology, 2015-08, Vol.176 (8), p.2225-2241</ispartof><rights>Springer Science+Business Media New York 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c512t-308f32d97d23e537e01388360c9775f36bd4741592f86d1ef015e058f44caea3</citedby><cites>FETCH-LOGICAL-c512t-308f32d97d23e537e01388360c9775f36bd4741592f86d1ef015e058f44caea3</cites><orcidid>0000-0002-9109-1248</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12010-015-1710-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12010-015-1710-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26100387$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Abdel-Aziz, Mohamed S.</creatorcontrib><creatorcontrib>Abou-El-Sherbini, Khaled S.</creatorcontrib><creatorcontrib>Hamzawy, Esmat M. A.</creatorcontrib><creatorcontrib>Amr, Mohey H. A.</creatorcontrib><creatorcontrib>El-Dafrawy, Shady</creatorcontrib><title>Green Synthesis of Silver Nano-particles by Macrococcus bovicus and Its Immobilization onto Montmorillonite Clay for Antimicrobial Functionality</title><title>Applied biochemistry and biotechnology</title><addtitle>Appl Biochem Biotechnol</addtitle><addtitle>Appl Biochem Biotechnol</addtitle><description>Macrococcus
bovicus
was locally isolated from soil and used in the green synthesis of nano-scaling silver (NSAg). It was immobilized on a sodic-montmorillonite clay (MMT1) and cetyltrimethylammonium bromide-modified montmorillonite (MMT2) which was also calcined at 300 °C (MMT3). The NSAg clays were characterized by X-ray fluorescence, Fourier transform infrared spectra, X-ray diffractometry, surface area measurement, UV–Vis spectrometry, scanning electron microscope, transmission electron microscope and thermogravimetric analysis. NSAg was confirmed to be included in the interparticular cavities of the clay sheets and its mechanical stability was evidenced. The antimicrobial activity of the NSAg-modified clays was investigated against
Staphylococcus aureus
,
Escherichia coli
and
Candida albicans
using the cup plate and the plate count techniques. The antimicrobial activity of the NSAg clays was confirmed and attributed to the caging of NSAg in MMT cavities. MMT3 was found to inhibit the microbial growth to as high as 65 % as observed from the plate count method.
Graphical Abstract
Scheme of the biosynthesis of nano-scaling Ag and its immobilization and antimicrobial application</description><subject>Aluminum Silicates - chemistry</subject><subject>Anti-Infective Agents - pharmacology</subject><subject>Antimicrobial agents</subject><subject>Bentonite - pharmacology</subject><subject>Biochemistry</subject><subject>Biosynthesis</subject><subject>Biotechnology</subject><subject>Candida albicans</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Clay</subject><subject>E coli</subject><subject>Escherichia coli</subject><subject>Fourier transforms</subject><subject>Green Chemistry Technology - methods</subject><subject>Metal Nanoparticles - chemistry</subject><subject>Metal Nanoparticles - ultrastructure</subject><subject>Microbial Sensitivity Tests</subject><subject>Montmorillonite</subject><subject>Nanoparticles</subject><subject>Silver</subject><subject>Silver - chemistry</subject><subject>Spectrometry</subject><subject>Spectrophotometry, Ultraviolet</subject><subject>Spectroscopy, Fourier Transform Infrared</subject><subject>Staphylococcaceae - drug effects</subject><subject>Staphylococcaceae - metabolism</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus aureus - drug effects</subject><subject>Thermogravimetric analysis</subject><subject>Thermogravimetry</subject><subject>X-Ray Diffraction</subject><subject>X-ray fluorescence</subject><issn>0273-2289</issn><issn>1559-0291</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqNkc9uFSEUxonR2Gv1AdwYEjduRvlzGZhlc9PWm7S6aPcThjkoDQNXYJqMT-Ejy820pjExccOB8Ps-Dt9B6C0lHykh8lOmjFDSECoaKuuGP0MbKkTXENbR52hDmOQNY6o7Qa9yviOEMiXkS3TC2qrnSm7Qr8sEEPDNEsp3yC7jaPGN8_eQ8BcdYnPQqTjjIeNhwdfapGiiMXM9xnt3rDqMeF8y3k9THJx3P3VxMeAYSsTXdZ1ict7H4ArgndcLtjHhs1Dc5KrZ4LTHF3MwR5H2riyv0QurfYY3D_UU3V6c3-4-N1dfL_e7s6vGCMpKw4mynI2dHBkHwSUQypXiLTGdlMLydhi3cktFx6xqRwq2hgREKLvdGg2an6IPq-0hxR8z5NJPLhvwXgeIc-5rnkxKzhT9D5TwtusEkRV9_xd6F-dUP7ZSlEulWKXoStUAck5g-0Nyk05LT0l_HGy_DravPR8bIT2vmncPzvMwwfhH8TjJCrAVyPUqfIP05Ol_uv4GDqCurA</recordid><startdate>20150801</startdate><enddate>20150801</enddate><creator>Abdel-Aziz, Mohamed S.</creator><creator>Abou-El-Sherbini, Khaled S.</creator><creator>Hamzawy, Esmat M. 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A.</au><au>Amr, Mohey H. A.</au><au>El-Dafrawy, Shady</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Green Synthesis of Silver Nano-particles by Macrococcus bovicus and Its Immobilization onto Montmorillonite Clay for Antimicrobial Functionality</atitle><jtitle>Applied biochemistry and biotechnology</jtitle><stitle>Appl Biochem Biotechnol</stitle><addtitle>Appl Biochem Biotechnol</addtitle><date>2015-08-01</date><risdate>2015</risdate><volume>176</volume><issue>8</issue><spage>2225</spage><epage>2241</epage><pages>2225-2241</pages><issn>0273-2289</issn><eissn>1559-0291</eissn><abstract>Macrococcus
bovicus
was locally isolated from soil and used in the green synthesis of nano-scaling silver (NSAg). It was immobilized on a sodic-montmorillonite clay (MMT1) and cetyltrimethylammonium bromide-modified montmorillonite (MMT2) which was also calcined at 300 °C (MMT3). The NSAg clays were characterized by X-ray fluorescence, Fourier transform infrared spectra, X-ray diffractometry, surface area measurement, UV–Vis spectrometry, scanning electron microscope, transmission electron microscope and thermogravimetric analysis. NSAg was confirmed to be included in the interparticular cavities of the clay sheets and its mechanical stability was evidenced. The antimicrobial activity of the NSAg-modified clays was investigated against
Staphylococcus aureus
,
Escherichia coli
and
Candida albicans
using the cup plate and the plate count techniques. The antimicrobial activity of the NSAg clays was confirmed and attributed to the caging of NSAg in MMT cavities. MMT3 was found to inhibit the microbial growth to as high as 65 % as observed from the plate count method.
Graphical Abstract
Scheme of the biosynthesis of nano-scaling Ag and its immobilization and antimicrobial application</abstract><cop>New York</cop><pub>Springer US</pub><pmid>26100387</pmid><doi>10.1007/s12010-015-1710-3</doi><tpages>17</tpages><orcidid>https://orcid.org/0000-0002-9109-1248</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0273-2289 |
| ispartof | Applied biochemistry and biotechnology, 2015-08, Vol.176 (8), p.2225-2241 |
| issn | 0273-2289 1559-0291 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1712773281 |
| source | MEDLINE; SpringerLink Journals |
| subjects | Aluminum Silicates - chemistry Anti-Infective Agents - pharmacology Antimicrobial agents Bentonite - pharmacology Biochemistry Biosynthesis Biotechnology Candida albicans Chemistry Chemistry and Materials Science Clay E coli Escherichia coli Fourier transforms Green Chemistry Technology - methods Metal Nanoparticles - chemistry Metal Nanoparticles - ultrastructure Microbial Sensitivity Tests Montmorillonite Nanoparticles Silver Silver - chemistry Spectrometry Spectrophotometry, Ultraviolet Spectroscopy, Fourier Transform Infrared Staphylococcaceae - drug effects Staphylococcaceae - metabolism Staphylococcus aureus Staphylococcus aureus - drug effects Thermogravimetric analysis Thermogravimetry X-Ray Diffraction X-ray fluorescence |
| title | Green Synthesis of Silver Nano-particles by Macrococcus bovicus and Its Immobilization onto Montmorillonite Clay for Antimicrobial Functionality |
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