A genome-wide functional assay of signal transduction in living mammalian cells

We describe a genome-wide functional assay for rapid isolation of cell clones and genetic elements responsive to specific stimuli. A promoterless β-lactamase reporter gene was transfected into a human T-cell line to generate a living library of reporter-tagged clones. When loaded with a cell-permeab...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Nature biotechnology 1998-12, Vol.16 (13), p.1329-1333
Hauptverfasser:	Whitney, Mike, Rockenstein, Edward, Cantin, Greg, Knapp, Tom, Zlokarnik, Gregor, Sanders, Pam, Durick, Kyle, Craig, Frank F., Negulescu, Paul A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Agriculture beta-Lactamases - genetics Bioinformatics Biological and medical sciences Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biomedicine Biotechnology Cell Separation Diverse techniques Enzyme Activation Flow Cytometry Fluorescent Dyes Fundamental and applied biological sciences. Psychology Genes, Reporter Genome Humans Jurkat Cells Life Sciences Molecular and cellular biology research-article Signal Transduction Tetradecanoylphorbol Acetate - pharmacology Thapsigargin - pharmacology
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!