The structure of PurR mutant L54M shows an alternative route to DNA kinking
The crystal structure of the purine repressor mutant L54M bound to hypoxanthine and to the purF operator provides a stereochemical understanding of the high DNA affinity of this hinge helix mutant. Comparison of the PurR L54M-DNA complex to that of the wild type PurR-DNA complex reveals that these p...
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| Veröffentlicht in: | Nature structural biology 1998-06, Vol.5 (6), p.436-441 |
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| container_title | Nature structural biology |
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| creator | Arvidson, Dennis N Lu, Fu Faber, Catherine Zalkin, Howard Brennan, Richard G |
| description | The crystal structure of the purine repressor mutant L54M bound to hypoxanthine and to the purF operator provides a stereochemical understanding of the high DNA affinity of this hinge helix mutant. Comparison of the PurR L54M-DNA complex to that of the wild type PurR-DNA complex reveals that these purine repressors bind and kink DNA similarly despite significant differences in their minor groove contacts and routes to interdigitation of the central C.G:G.C base pair step. Modeling studies, supported by genetic and biochemical data, show that the stereochemistry of the backbone atoms of the abutting hinge helices combined with the rigidity of the kinked base pair step constrain the interdigitating residue to leucine or methionine for the LacI/GalR family of transcription regulators. |
| doi_str_mv | 10.1038/nsb0698-436 |
| format | Article |
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Comparison of the PurR L54M-DNA complex to that of the wild type PurR-DNA complex reveals that these purine repressors bind and kink DNA similarly despite significant differences in their minor groove contacts and routes to interdigitation of the central C.G:G.C base pair step. 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| ispartof | Nature structural biology, 1998-06, Vol.5 (6), p.436-441 |
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| source | MEDLINE; Nature; Alma/SFX Local Collection |
| subjects | Amino Acid Substitution - genetics Bacterial Proteins - chemistry Bacterial Proteins - genetics Crystallography, X-Ray DNA-Binding Proteins - chemistry DNA-Binding Proteins - genetics Leucine - genetics Methionine - genetics Models, Molecular Mutagenesis, Site-Directed Nucleic Acid Conformation Protein Binding Protein Folding Protein Structure, Secondary Repressor Proteins - chemistry Repressor Proteins - genetics |
| title | The structure of PurR mutant L54M shows an alternative route to DNA kinking |
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