Phenolic compounds from the bark of Oroxylum indicum activate the Ngn2 promoter
A reporter gene assay that detects neurogenin 2 (Ngn2) promoter activity was utilized to identify compounds that induce neuronal differentiation. Ngn2 is a basic helix-loop-helix transcription factor that activates transcription of pro-neural genes. Using this assay system and an activity-guided app...
Gespeichert in:
| Veröffentlicht in: | Journal of natural medicines 2015-10, Vol.69 (4), p.589-594 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 594 |
|---|---|
| container_issue | 4 |
| container_start_page | 589 |
| container_title | Journal of natural medicines |
| container_volume | 69 |
| creator | Fuentes, Rolly G Arai, Midori A Sadhu, Samir K Ahmed, Firoj Ishibashi, Masami |
| description | A reporter gene assay that detects neurogenin 2 (Ngn2) promoter activity was utilized to identify compounds that induce neuronal differentiation. Ngn2 is a basic helix-loop-helix transcription factor that activates transcription of pro-neural genes. Using this assay system and an activity-guided approach, seven phenolic compounds were isolated from the methanol extract of Oroxylum indicum: 1 oroxylin A, 2 chrysin, 3 hispidulin, 4 baicalein, 5 apigenin, 6 baicalin, and 7 isoverbascoside. Compounds 1 and 2 induced an estimated 2.7-fold increase in Ngn2 promoter activity, whereas 3 increased the activity by 2.5-fold. Furthermore, 1 and 2 enhanced neuronal differentiation of C17.2 cells, which are multipotent stem cells. |
| doi_str_mv | 10.1007/s11418-015-0919-3 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1710656264</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1710656264</sourcerecordid><originalsourceid>FETCH-LOGICAL-c555t-e0b8ec597ab98b952b8c449c73356d070485446afd4cb7909a12ef2c9426af443</originalsourceid><addsrcrecordid>eNp9kM1O3DAURq2qqAO0D9ANZNlN4F7_JV5WqC1II6ZSO2vLcZyZTJN4sBPEvD2eBliyupZ9vs-6h5CvCFcIUFxHRI5lDihyUKhy9oGcYikxB6rYx3RmHHLGOVuQsxh3AJwyhp_IgkpADlycktXvrRt819rM-n7vp6GOWRN8n41bl1Um_Mt8k62Cfzp0U5-1Q93aNI0d20czuv_U_Wag2T5l_OjCZ3LSmC66Ly_znKx__vh7c5svV7_ubr4vcyuEGHMHVemsUIWpVFkpQavScq5swZiQNRTAS8G5NE3NbVUoUAapa6hVnKbLtNE5-Tb3po8fJhdH3bfRuq4zg_NT1FggSCGpPKI4ozb4GINr9D60vQkHjaCPHvXsUSeP-uhRs5S5eKmfqt7Vb4lXcQmgMxDT07BxQe_8FIa08rutl3OoMV6bTWijXv-hgBIAKBRSsWfrOYYB</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1710656264</pqid></control><display><type>article</type><title>Phenolic compounds from the bark of Oroxylum indicum activate the Ngn2 promoter</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Fuentes, Rolly G ; Arai, Midori A ; Sadhu, Samir K ; Ahmed, Firoj ; Ishibashi, Masami</creator><creatorcontrib>Fuentes, Rolly G ; Arai, Midori A ; Sadhu, Samir K ; Ahmed, Firoj ; Ishibashi, Masami</creatorcontrib><description>A reporter gene assay that detects neurogenin 2 (Ngn2) promoter activity was utilized to identify compounds that induce neuronal differentiation. Ngn2 is a basic helix-loop-helix transcription factor that activates transcription of pro-neural genes. Using this assay system and an activity-guided approach, seven phenolic compounds were isolated from the methanol extract of Oroxylum indicum: 1 oroxylin A, 2 chrysin, 3 hispidulin, 4 baicalein, 5 apigenin, 6 baicalin, and 7 isoverbascoside. Compounds 1 and 2 induced an estimated 2.7-fold increase in Ngn2 promoter activity, whereas 3 increased the activity by 2.5-fold. Furthermore, 1 and 2 enhanced neuronal differentiation of C17.2 cells, which are multipotent stem cells.</description><identifier>ISSN: 1340-3443</identifier><identifier>EISSN: 1861-0293</identifier><identifier>DOI: 10.1007/s11418-015-0919-3</identifier><identifier>PMID: 26014045</identifier><language>eng</language><publisher>Tokyo: Springer Japan</publisher><subject>apigenin ; baicalin ; bark ; basic helix-loop-helix transcription factors ; Basic Helix-Loop-Helix Transcription Factors - genetics ; Biomedical and Life Sciences ; Biomedicine ; Complementary & Alternative Medicine ; Medicinal Chemistry ; methanol ; Molecular Structure ; Nerve Tissue Proteins - genetics ; Oroxylum indicum ; Pharmacology/Toxicology ; Pharmacy ; phenolic compounds ; Phenols - analysis ; Phenols - chemistry ; Plant Extracts - chemistry ; Plant Extracts - pharmacology ; Plant Sciences ; reporter genes ; stem cells ; Transcription Factors - genetics</subject><ispartof>Journal of natural medicines, 2015-10, Vol.69 (4), p.589-594</ispartof><rights>The Japanese Society of Pharmacognosy and Springer Japan 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c555t-e0b8ec597ab98b952b8c449c73356d070485446afd4cb7909a12ef2c9426af443</citedby><cites>FETCH-LOGICAL-c555t-e0b8ec597ab98b952b8c449c73356d070485446afd4cb7909a12ef2c9426af443</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11418-015-0919-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11418-015-0919-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26014045$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fuentes, Rolly G</creatorcontrib><creatorcontrib>Arai, Midori A</creatorcontrib><creatorcontrib>Sadhu, Samir K</creatorcontrib><creatorcontrib>Ahmed, Firoj</creatorcontrib><creatorcontrib>Ishibashi, Masami</creatorcontrib><title>Phenolic compounds from the bark of Oroxylum indicum activate the Ngn2 promoter</title><title>Journal of natural medicines</title><addtitle>J Nat Med</addtitle><addtitle>J Nat Med</addtitle><description>A reporter gene assay that detects neurogenin 2 (Ngn2) promoter activity was utilized to identify compounds that induce neuronal differentiation. Ngn2 is a basic helix-loop-helix transcription factor that activates transcription of pro-neural genes. Using this assay system and an activity-guided approach, seven phenolic compounds were isolated from the methanol extract of Oroxylum indicum: 1 oroxylin A, 2 chrysin, 3 hispidulin, 4 baicalein, 5 apigenin, 6 baicalin, and 7 isoverbascoside. Compounds 1 and 2 induced an estimated 2.7-fold increase in Ngn2 promoter activity, whereas 3 increased the activity by 2.5-fold. Furthermore, 1 and 2 enhanced neuronal differentiation of C17.2 cells, which are multipotent stem cells.</description><subject>apigenin</subject><subject>baicalin</subject><subject>bark</subject><subject>basic helix-loop-helix transcription factors</subject><subject>Basic Helix-Loop-Helix Transcription Factors - genetics</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Complementary & Alternative Medicine</subject><subject>Medicinal Chemistry</subject><subject>methanol</subject><subject>Molecular Structure</subject><subject>Nerve Tissue Proteins - genetics</subject><subject>Oroxylum indicum</subject><subject>Pharmacology/Toxicology</subject><subject>Pharmacy</subject><subject>phenolic compounds</subject><subject>Phenols - analysis</subject><subject>Phenols - chemistry</subject><subject>Plant Extracts - chemistry</subject><subject>Plant Extracts - pharmacology</subject><subject>Plant Sciences</subject><subject>reporter genes</subject><subject>stem cells</subject><subject>Transcription Factors - genetics</subject><issn>1340-3443</issn><issn>1861-0293</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM1O3DAURq2qqAO0D9ANZNlN4F7_JV5WqC1II6ZSO2vLcZyZTJN4sBPEvD2eBliyupZ9vs-6h5CvCFcIUFxHRI5lDihyUKhy9oGcYikxB6rYx3RmHHLGOVuQsxh3AJwyhp_IgkpADlycktXvrRt819rM-n7vp6GOWRN8n41bl1Um_Mt8k62Cfzp0U5-1Q93aNI0d20czuv_U_Wag2T5l_OjCZ3LSmC66Ly_znKx__vh7c5svV7_ubr4vcyuEGHMHVemsUIWpVFkpQavScq5swZiQNRTAS8G5NE3NbVUoUAapa6hVnKbLtNE5-Tb3po8fJhdH3bfRuq4zg_NT1FggSCGpPKI4ozb4GINr9D60vQkHjaCPHvXsUSeP-uhRs5S5eKmfqt7Vb4lXcQmgMxDT07BxQe_8FIa08rutl3OoMV6bTWijXv-hgBIAKBRSsWfrOYYB</recordid><startdate>20151001</startdate><enddate>20151001</enddate><creator>Fuentes, Rolly G</creator><creator>Arai, Midori A</creator><creator>Sadhu, Samir K</creator><creator>Ahmed, Firoj</creator><creator>Ishibashi, Masami</creator><general>Springer Japan</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20151001</creationdate><title>Phenolic compounds from the bark of Oroxylum indicum activate the Ngn2 promoter</title><author>Fuentes, Rolly G ; Arai, Midori A ; Sadhu, Samir K ; Ahmed, Firoj ; Ishibashi, Masami</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c555t-e0b8ec597ab98b952b8c449c73356d070485446afd4cb7909a12ef2c9426af443</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>apigenin</topic><topic>baicalin</topic><topic>bark</topic><topic>basic helix-loop-helix transcription factors</topic><topic>Basic Helix-Loop-Helix Transcription Factors - genetics</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Complementary & Alternative Medicine</topic><topic>Medicinal Chemistry</topic><topic>methanol</topic><topic>Molecular Structure</topic><topic>Nerve Tissue Proteins - genetics</topic><topic>Oroxylum indicum</topic><topic>Pharmacology/Toxicology</topic><topic>Pharmacy</topic><topic>phenolic compounds</topic><topic>Phenols - analysis</topic><topic>Phenols - chemistry</topic><topic>Plant Extracts - chemistry</topic><topic>Plant Extracts - pharmacology</topic><topic>Plant Sciences</topic><topic>reporter genes</topic><topic>stem cells</topic><topic>Transcription Factors - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fuentes, Rolly G</creatorcontrib><creatorcontrib>Arai, Midori A</creatorcontrib><creatorcontrib>Sadhu, Samir K</creatorcontrib><creatorcontrib>Ahmed, Firoj</creatorcontrib><creatorcontrib>Ishibashi, Masami</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of natural medicines</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fuentes, Rolly G</au><au>Arai, Midori A</au><au>Sadhu, Samir K</au><au>Ahmed, Firoj</au><au>Ishibashi, Masami</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phenolic compounds from the bark of Oroxylum indicum activate the Ngn2 promoter</atitle><jtitle>Journal of natural medicines</jtitle><stitle>J Nat Med</stitle><addtitle>J Nat Med</addtitle><date>2015-10-01</date><risdate>2015</risdate><volume>69</volume><issue>4</issue><spage>589</spage><epage>594</epage><pages>589-594</pages><issn>1340-3443</issn><eissn>1861-0293</eissn><abstract>A reporter gene assay that detects neurogenin 2 (Ngn2) promoter activity was utilized to identify compounds that induce neuronal differentiation. Ngn2 is a basic helix-loop-helix transcription factor that activates transcription of pro-neural genes. Using this assay system and an activity-guided approach, seven phenolic compounds were isolated from the methanol extract of Oroxylum indicum: 1 oroxylin A, 2 chrysin, 3 hispidulin, 4 baicalein, 5 apigenin, 6 baicalin, and 7 isoverbascoside. Compounds 1 and 2 induced an estimated 2.7-fold increase in Ngn2 promoter activity, whereas 3 increased the activity by 2.5-fold. Furthermore, 1 and 2 enhanced neuronal differentiation of C17.2 cells, which are multipotent stem cells.</abstract><cop>Tokyo</cop><pub>Springer Japan</pub><pmid>26014045</pmid><doi>10.1007/s11418-015-0919-3</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1340-3443 |
| ispartof | Journal of natural medicines, 2015-10, Vol.69 (4), p.589-594 |
| issn | 1340-3443 1861-0293 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1710656264 |
| source | MEDLINE; SpringerLink Journals |
| subjects | apigenin baicalin bark basic helix-loop-helix transcription factors Basic Helix-Loop-Helix Transcription Factors - genetics Biomedical and Life Sciences Biomedicine Complementary & Alternative Medicine Medicinal Chemistry methanol Molecular Structure Nerve Tissue Proteins - genetics Oroxylum indicum Pharmacology/Toxicology Pharmacy phenolic compounds Phenols - analysis Phenols - chemistry Plant Extracts - chemistry Plant Extracts - pharmacology Plant Sciences reporter genes stem cells Transcription Factors - genetics |
| title | Phenolic compounds from the bark of Oroxylum indicum activate the Ngn2 promoter |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-09T12%3A49%3A34IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Phenolic%20compounds%20from%20the%20bark%20of%20Oroxylum%20indicum%20activate%20the%20Ngn2%20promoter&rft.jtitle=Journal%20of%20natural%20medicines&rft.au=Fuentes,%20Rolly%20G&rft.date=2015-10-01&rft.volume=69&rft.issue=4&rft.spage=589&rft.epage=594&rft.pages=589-594&rft.issn=1340-3443&rft.eissn=1861-0293&rft_id=info:doi/10.1007/s11418-015-0919-3&rft_dat=%3Cproquest_cross%3E1710656264%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1710656264&rft_id=info:pmid/26014045&rfr_iscdi=true |