Evaluation of a novel multiplex RT-qPCR assay for the quantification of leukemia-associated BCR-ABL1 translocation
Although monitoring of BCR-ABL1 translocation has become an established practice in the management of chronic myeloid leukemia (CML), the detection limit of the BCR-ABL1 transcripts needs more standardization. The aim of the present study was to evaluate the clinical performances of a novel assay fo...
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| Veröffentlicht in: | International journal of hematology 2015-09, Vol.102 (3), p.335-341 |
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| creator | Kottwitz, D. EL Hadi, H. El Amrani, M. Cabezas, S. Dehbi, H. Nadifi, S. Quessar, A. Colomer, D. Moumen, Abdeladim Sefrioui, EL Hassan |
| description | Although monitoring of BCR-ABL1 translocation has become an established practice in the management of chronic myeloid leukemia (CML), the detection limit of the BCR-ABL1 transcripts needs more standardization. The aim of the present study was to evaluate the clinical performances of a novel assay for the quantification of BCR-ABL1 fusion transcripts (e13a2 and e14a2) and ABL1 in a single reaction. This assay is based on the real-time reverse transcription polymerase chain reaction (RT-qPCR) in multiplex format. In a retrospective comparative clinical study performed in a reference laboratory, RNA was extracted from 48 CML patient blood samples with various BCR-ABL1/ABL1 ratios and RT-qPCR was performed using either MAScIR assay or the RT-qPCR simplex reference assay used in routine clinical testing. The comparative clinical results showed high qualitative and quantitative concordance (correlation coefficient >0.95) between MAScIR and the reference assays. The present study illustrates the utility of MAScIR assay as a sensitive, rapid, and cost-effective quantitative device to monitor the BCR-ABL1 ratios by RT-qPCR on whole blood of diagnosed Philadelphia chromosome-positive (Ph+) leukemia patients. This test could be used as an aid in the assessment of molecular response to available treatments. |
| doi_str_mv | 10.1007/s12185-015-1839-4 |
| format | Article |
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The aim of the present study was to evaluate the clinical performances of a novel assay for the quantification of BCR-ABL1 fusion transcripts (e13a2 and e14a2) and ABL1 in a single reaction. This assay is based on the real-time reverse transcription polymerase chain reaction (RT-qPCR) in multiplex format. In a retrospective comparative clinical study performed in a reference laboratory, RNA was extracted from 48 CML patient blood samples with various BCR-ABL1/ABL1 ratios and RT-qPCR was performed using either MAScIR assay or the RT-qPCR simplex reference assay used in routine clinical testing. The comparative clinical results showed high qualitative and quantitative concordance (correlation coefficient >0.95) between MAScIR and the reference assays. The present study illustrates the utility of MAScIR assay as a sensitive, rapid, and cost-effective quantitative device to monitor the BCR-ABL1 ratios by RT-qPCR on whole blood of diagnosed Philadelphia chromosome-positive (Ph+) leukemia patients. This test could be used as an aid in the assessment of molecular response to available treatments.</description><identifier>ISSN: 0925-5710</identifier><identifier>EISSN: 1865-3774</identifier><identifier>DOI: 10.1007/s12185-015-1839-4</identifier><identifier>PMID: 26243622</identifier><language>eng</language><publisher>Tokyo: Springer Japan</publisher><subject>Female ; Fusion Proteins, bcr-abl - blood ; Fusion Proteins, bcr-abl - genetics ; Hematology ; Humans ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive - blood ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive - genetics ; Male ; Medicine ; Medicine & Public Health ; Multiplex Polymerase Chain Reaction - methods ; Oncology ; Original Article ; Philadelphia Chromosome ; Retrospective Studies ; Reverse Transcriptase Polymerase Chain Reaction - methods ; RNA, Messenger - blood ; RNA, Messenger - genetics ; Translocation, Genetic</subject><ispartof>International journal of hematology, 2015-09, Vol.102 (3), p.335-341</ispartof><rights>The Japanese Society of Hematology 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c466t-5b598716bd7b7a60ab6e8e84bc765327227dbb8c008218cc25e744fbc549c0a63</citedby><cites>FETCH-LOGICAL-c466t-5b598716bd7b7a60ab6e8e84bc765327227dbb8c008218cc25e744fbc549c0a63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12185-015-1839-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12185-015-1839-4$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27922,27923,41486,42555,51317</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26243622$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kottwitz, D.</creatorcontrib><creatorcontrib>EL Hadi, H.</creatorcontrib><creatorcontrib>El Amrani, M.</creatorcontrib><creatorcontrib>Cabezas, S.</creatorcontrib><creatorcontrib>Dehbi, H.</creatorcontrib><creatorcontrib>Nadifi, S.</creatorcontrib><creatorcontrib>Quessar, A.</creatorcontrib><creatorcontrib>Colomer, D.</creatorcontrib><creatorcontrib>Moumen, Abdeladim</creatorcontrib><creatorcontrib>Sefrioui, EL Hassan</creatorcontrib><title>Evaluation of a novel multiplex RT-qPCR assay for the quantification of leukemia-associated BCR-ABL1 translocation</title><title>International journal of hematology</title><addtitle>Int J Hematol</addtitle><addtitle>Int J Hematol</addtitle><description>Although monitoring of BCR-ABL1 translocation has become an established practice in the management of chronic myeloid leukemia (CML), the detection limit of the BCR-ABL1 transcripts needs more standardization. The aim of the present study was to evaluate the clinical performances of a novel assay for the quantification of BCR-ABL1 fusion transcripts (e13a2 and e14a2) and ABL1 in a single reaction. This assay is based on the real-time reverse transcription polymerase chain reaction (RT-qPCR) in multiplex format. In a retrospective comparative clinical study performed in a reference laboratory, RNA was extracted from 48 CML patient blood samples with various BCR-ABL1/ABL1 ratios and RT-qPCR was performed using either MAScIR assay or the RT-qPCR simplex reference assay used in routine clinical testing. The comparative clinical results showed high qualitative and quantitative concordance (correlation coefficient >0.95) between MAScIR and the reference assays. The present study illustrates the utility of MAScIR assay as a sensitive, rapid, and cost-effective quantitative device to monitor the BCR-ABL1 ratios by RT-qPCR on whole blood of diagnosed Philadelphia chromosome-positive (Ph+) leukemia patients. This test could be used as an aid in the assessment of molecular response to available treatments.</description><subject>Female</subject><subject>Fusion Proteins, bcr-abl - blood</subject><subject>Fusion Proteins, bcr-abl - genetics</subject><subject>Hematology</subject><subject>Humans</subject><subject>Leukemia, Myelogenous, Chronic, BCR-ABL Positive - blood</subject><subject>Leukemia, Myelogenous, Chronic, BCR-ABL Positive - genetics</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Multiplex Polymerase Chain Reaction - methods</subject><subject>Oncology</subject><subject>Original Article</subject><subject>Philadelphia Chromosome</subject><subject>Retrospective Studies</subject><subject>Reverse Transcriptase Polymerase Chain Reaction - methods</subject><subject>RNA, Messenger - blood</subject><subject>RNA, Messenger - genetics</subject><subject>Translocation, Genetic</subject><issn>0925-5710</issn><issn>1865-3774</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNp1kU1LxDAURYMoOo7-ADcScOMmmqT5aJc6-AUDyqDrkGRSrabNTNKK_nsjVRHBVRbv3PvCOwAcEHxCMJaniVBScoQJR6QsKsQ2wISUgqNCSrYJJriiHHFJ8A7YTekZYyIxk9tghwrKCkHpBMSLV-0H3Tehg6GGGnbh1XnYDr5vVt69wcU9Wt_NFlCnpN9hHSLsnxxcD7rrm7qxP0nvhhfXNhplMNhG924Jz2cLdHY-J7CPuks-jPQe2Kq1T27_652Ch8uL-9k1mt9e3czO5sgyIXrEDa9KSYRZSiO1wNoIV7qSGSsFL6ikVC6NKS3GZb6CtZQ7yVhtLGeVxVoUU3A89q5iWA8u9aptknXe686FISmSDyM4ZRRn9OgP-hyG2OXfZQpXleQs75wCMlI2hpSiq9UqNq2O74pg9SlEjUJUFqI-hSiWM4dfzYNp3fIn8W0gA3QEUh51jy7-Wv1v6wfMvZUy</recordid><startdate>20150901</startdate><enddate>20150901</enddate><creator>Kottwitz, D.</creator><creator>EL Hadi, H.</creator><creator>El Amrani, M.</creator><creator>Cabezas, S.</creator><creator>Dehbi, H.</creator><creator>Nadifi, S.</creator><creator>Quessar, A.</creator><creator>Colomer, D.</creator><creator>Moumen, Abdeladim</creator><creator>Sefrioui, EL Hassan</creator><general>Springer Japan</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M1P</scope><scope>NAPCQ</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope></search><sort><creationdate>20150901</creationdate><title>Evaluation of a novel multiplex RT-qPCR assay for the quantification of leukemia-associated BCR-ABL1 translocation</title><author>Kottwitz, D. ; EL Hadi, H. ; El Amrani, M. ; Cabezas, S. ; Dehbi, H. ; Nadifi, S. ; Quessar, A. ; Colomer, D. ; Moumen, Abdeladim ; Sefrioui, EL Hassan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c466t-5b598716bd7b7a60ab6e8e84bc765327227dbb8c008218cc25e744fbc549c0a63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Female</topic><topic>Fusion Proteins, bcr-abl - blood</topic><topic>Fusion Proteins, bcr-abl - genetics</topic><topic>Hematology</topic><topic>Humans</topic><topic>Leukemia, Myelogenous, Chronic, BCR-ABL Positive - blood</topic><topic>Leukemia, Myelogenous, Chronic, BCR-ABL Positive - genetics</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Multiplex Polymerase Chain Reaction - methods</topic><topic>Oncology</topic><topic>Original Article</topic><topic>Philadelphia Chromosome</topic><topic>Retrospective Studies</topic><topic>Reverse Transcriptase Polymerase Chain Reaction - methods</topic><topic>RNA, Messenger - blood</topic><topic>RNA, Messenger - genetics</topic><topic>Translocation, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kottwitz, D.</creatorcontrib><creatorcontrib>EL Hadi, H.</creatorcontrib><creatorcontrib>El Amrani, M.</creatorcontrib><creatorcontrib>Cabezas, S.</creatorcontrib><creatorcontrib>Dehbi, H.</creatorcontrib><creatorcontrib>Nadifi, S.</creatorcontrib><creatorcontrib>Quessar, A.</creatorcontrib><creatorcontrib>Colomer, D.</creatorcontrib><creatorcontrib>Moumen, Abdeladim</creatorcontrib><creatorcontrib>Sefrioui, EL Hassan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of hematology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kottwitz, D.</au><au>EL Hadi, H.</au><au>El Amrani, M.</au><au>Cabezas, S.</au><au>Dehbi, H.</au><au>Nadifi, S.</au><au>Quessar, A.</au><au>Colomer, D.</au><au>Moumen, Abdeladim</au><au>Sefrioui, EL Hassan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of a novel multiplex RT-qPCR assay for the quantification of leukemia-associated BCR-ABL1 translocation</atitle><jtitle>International journal of hematology</jtitle><stitle>Int J Hematol</stitle><addtitle>Int J Hematol</addtitle><date>2015-09-01</date><risdate>2015</risdate><volume>102</volume><issue>3</issue><spage>335</spage><epage>341</epage><pages>335-341</pages><issn>0925-5710</issn><eissn>1865-3774</eissn><abstract>Although monitoring of BCR-ABL1 translocation has become an established practice in the management of chronic myeloid leukemia (CML), the detection limit of the BCR-ABL1 transcripts needs more standardization. The aim of the present study was to evaluate the clinical performances of a novel assay for the quantification of BCR-ABL1 fusion transcripts (e13a2 and e14a2) and ABL1 in a single reaction. This assay is based on the real-time reverse transcription polymerase chain reaction (RT-qPCR) in multiplex format. In a retrospective comparative clinical study performed in a reference laboratory, RNA was extracted from 48 CML patient blood samples with various BCR-ABL1/ABL1 ratios and RT-qPCR was performed using either MAScIR assay or the RT-qPCR simplex reference assay used in routine clinical testing. The comparative clinical results showed high qualitative and quantitative concordance (correlation coefficient >0.95) between MAScIR and the reference assays. The present study illustrates the utility of MAScIR assay as a sensitive, rapid, and cost-effective quantitative device to monitor the BCR-ABL1 ratios by RT-qPCR on whole blood of diagnosed Philadelphia chromosome-positive (Ph+) leukemia patients. This test could be used as an aid in the assessment of molecular response to available treatments.</abstract><cop>Tokyo</cop><pub>Springer Japan</pub><pmid>26243622</pmid><doi>10.1007/s12185-015-1839-4</doi><tpages>7</tpages></addata></record> |
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| subjects | Female Fusion Proteins, bcr-abl - blood Fusion Proteins, bcr-abl - genetics Hematology Humans Leukemia, Myelogenous, Chronic, BCR-ABL Positive - blood Leukemia, Myelogenous, Chronic, BCR-ABL Positive - genetics Male Medicine Medicine & Public Health Multiplex Polymerase Chain Reaction - methods Oncology Original Article Philadelphia Chromosome Retrospective Studies Reverse Transcriptase Polymerase Chain Reaction - methods RNA, Messenger - blood RNA, Messenger - genetics Translocation, Genetic |
| title | Evaluation of a novel multiplex RT-qPCR assay for the quantification of leukemia-associated BCR-ABL1 translocation |
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