Purification and characterization of chitinases from transformed callus suspension cultures of Trichosanthes kirilowii Maxim

Three extracellular basic chitinases designated as TKC 15, TKC 28-I, and TKC 28-II were purified from Agrobacterium rhizogenes A4 transformed Trichosanthes kirilowii Maxim. callus suspension cultures using PerSeptive HS M cation exchange and Sephadex G 75 S gel filtration chromatography. These chiti...

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Veröffentlicht in:	Journal of fermentation and bioengineering 1997-01, Vol.84 (1), p.28-34
Hauptverfasser:	Remi Shih, N.-J., McDonald, Karen A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Agrobacterium rhizogenes Aspergillus flavus Biological and medical sciences Biotechnology CAL CALLO CALLUS CELL CULTURE CHITINASE chitinases CULTIVO DE CELULAS CULTURE DE CELLULE Enzyme engineering Fundamental and applied biological sciences. Psychology Improved methods for extraction and purification of enzymes Methods. Procedures. Technologies plant cell suspension cultures PURIFICACION PURIFICATION QUITINASA Trichoderma viride TRICHOSANTHES Trichosanthes kirilowii
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