Exposure to a Low Lead Concentration Impairs Contractile Machinery in Rat Cardiac Muscle
Lead exposure has been considered to be a risk factor for hypertension and cardiovascular disease. Our purpose was to evaluate the effects of low plasma lead concentration on cardiac contractility in isolated papillary muscles. Wistar rats were divided in control group or group treated with 100 ppm...
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| Veröffentlicht in: | Biological trace element research 2015-10, Vol.167 (2), p.280-287 |
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| creator | Silva, Marito A. S. C de Oliveira, Thiago F Almenara, Camila C. P Broseghini-Filho, Gilson B Vassallo, Dalton V Padilha, Alessandra S Silveira, Edna A |
| description | Lead exposure has been considered to be a risk factor for hypertension and cardiovascular disease. Our purpose was to evaluate the effects of low plasma lead concentration on cardiac contractility in isolated papillary muscles. Wistar rats were divided in control group or group treated with 100 ppm of lead acetate in the drinking water for 15 days. Blood pressure (BP) was measured weekly. At the end of the treatment period, the animals were anesthetized and euthanized, and parameters related to isolated papillary muscle contractility were recorded. The lead concentrations in the blood reached 12.3 ± 2 μg/dL. The BP was increased in the group treated with 100 ppm of lead acetate. Lead treatment did not alter force and time derivatives of the force of left ventricular papillary muscles. In addition, the inotropic response induced by an increase in the extracellular Ca²⁺ concentration was reduced in the Pb²⁺ group. However, the uptake of Ca²⁺ by the sarcoplasmic reticulum and the protein expression of SERCA and phospholamban remained unchanged. Postrest contraction was similar in the both groups, and tetanic peak and plateau tension were reduced in lead group. These results demonstrated that the reduction in the inotropic response to calcium does not appear to be caused by changes in the trans-sarcolemmal calcium flux but suggest that an impairment of the contractile machinery might be taking place. Our results demonstrate that even at a concentration below the limit considered to be safe, lead exerts deleterious effects on the cardiac contractile machinery. |
| doi_str_mv | 10.1007/s12011-015-0300-0 |
| format | Article |
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S. C ; de Oliveira, Thiago F ; Almenara, Camila C. P ; Broseghini-Filho, Gilson B ; Vassallo, Dalton V ; Padilha, Alessandra S ; Silveira, Edna A</creator><creatorcontrib>Silva, Marito A. S. C ; de Oliveira, Thiago F ; Almenara, Camila C. P ; Broseghini-Filho, Gilson B ; Vassallo, Dalton V ; Padilha, Alessandra S ; Silveira, Edna A</creatorcontrib><description>Lead exposure has been considered to be a risk factor for hypertension and cardiovascular disease. Our purpose was to evaluate the effects of low plasma lead concentration on cardiac contractility in isolated papillary muscles. Wistar rats were divided in control group or group treated with 100 ppm of lead acetate in the drinking water for 15 days. Blood pressure (BP) was measured weekly. At the end of the treatment period, the animals were anesthetized and euthanized, and parameters related to isolated papillary muscle contractility were recorded. The lead concentrations in the blood reached 12.3 ± 2 μg/dL. The BP was increased in the group treated with 100 ppm of lead acetate. Lead treatment did not alter force and time derivatives of the force of left ventricular papillary muscles. In addition, the inotropic response induced by an increase in the extracellular Ca²⁺ concentration was reduced in the Pb²⁺ group. However, the uptake of Ca²⁺ by the sarcoplasmic reticulum and the protein expression of SERCA and phospholamban remained unchanged. Postrest contraction was similar in the both groups, and tetanic peak and plateau tension were reduced in lead group. These results demonstrated that the reduction in the inotropic response to calcium does not appear to be caused by changes in the trans-sarcolemmal calcium flux but suggest that an impairment of the contractile machinery might be taking place. Our results demonstrate that even at a concentration below the limit considered to be safe, lead exerts deleterious effects on the cardiac contractile machinery.</description><identifier>ISSN: 0163-4984</identifier><identifier>EISSN: 1559-0720</identifier><identifier>DOI: 10.1007/s12011-015-0300-0</identifier><identifier>PMID: 25795172</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>acetates ; Animals ; Biochemistry ; Biomedical and Life Sciences ; Biotechnology ; blood ; Blood pressure ; Blood Pressure - drug effects ; Calcium ; Calcium-Binding Proteins - biosynthesis ; Cardiomyocytes ; Cardiovascular diseases ; Drinking water ; groups ; Hypertension ; Lead ; lead acetate ; Lead Poisoning - metabolism ; Lead Poisoning - physiopathology ; Life Sciences ; machinery ; Male ; muscle strength ; Muscles ; Myocardial Contraction - drug effects ; myocardium ; Myocardium - metabolism ; Nutrition ; Oncology ; Organometallic Compounds - toxicity ; protein synthesis ; Proteins ; Rats ; Rats, Wistar ; Risk factors ; Rodents ; sarcoplasmic reticulum ; Sarcoplasmic Reticulum Calcium-Transporting ATPases - biosynthesis</subject><ispartof>Biological trace element research, 2015-10, Vol.167 (2), p.280-287</ispartof><rights>Springer Science+Business Media New York 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c532t-775a1715f1020bf314dbbe7ad84f51c6398ad49ed5fef9450ac3556356a6d5aa3</citedby><cites>FETCH-LOGICAL-c532t-775a1715f1020bf314dbbe7ad84f51c6398ad49ed5fef9450ac3556356a6d5aa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12011-015-0300-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12011-015-0300-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25795172$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Silva, Marito A. S. C</creatorcontrib><creatorcontrib>de Oliveira, Thiago F</creatorcontrib><creatorcontrib>Almenara, Camila C. P</creatorcontrib><creatorcontrib>Broseghini-Filho, Gilson B</creatorcontrib><creatorcontrib>Vassallo, Dalton V</creatorcontrib><creatorcontrib>Padilha, Alessandra S</creatorcontrib><creatorcontrib>Silveira, Edna A</creatorcontrib><title>Exposure to a Low Lead Concentration Impairs Contractile Machinery in Rat Cardiac Muscle</title><title>Biological trace element research</title><addtitle>Biol Trace Elem Res</addtitle><addtitle>Biol Trace Elem Res</addtitle><description>Lead exposure has been considered to be a risk factor for hypertension and cardiovascular disease. Our purpose was to evaluate the effects of low plasma lead concentration on cardiac contractility in isolated papillary muscles. Wistar rats were divided in control group or group treated with 100 ppm of lead acetate in the drinking water for 15 days. Blood pressure (BP) was measured weekly. At the end of the treatment period, the animals were anesthetized and euthanized, and parameters related to isolated papillary muscle contractility were recorded. The lead concentrations in the blood reached 12.3 ± 2 μg/dL. The BP was increased in the group treated with 100 ppm of lead acetate. Lead treatment did not alter force and time derivatives of the force of left ventricular papillary muscles. In addition, the inotropic response induced by an increase in the extracellular Ca²⁺ concentration was reduced in the Pb²⁺ group. However, the uptake of Ca²⁺ by the sarcoplasmic reticulum and the protein expression of SERCA and phospholamban remained unchanged. Postrest contraction was similar in the both groups, and tetanic peak and plateau tension were reduced in lead group. These results demonstrated that the reduction in the inotropic response to calcium does not appear to be caused by changes in the trans-sarcolemmal calcium flux but suggest that an impairment of the contractile machinery might be taking place. Our results demonstrate that even at a concentration below the limit considered to be safe, lead exerts deleterious effects on the cardiac contractile machinery.</description><subject>acetates</subject><subject>Animals</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>blood</subject><subject>Blood pressure</subject><subject>Blood Pressure - drug effects</subject><subject>Calcium</subject><subject>Calcium-Binding Proteins - biosynthesis</subject><subject>Cardiomyocytes</subject><subject>Cardiovascular diseases</subject><subject>Drinking water</subject><subject>groups</subject><subject>Hypertension</subject><subject>Lead</subject><subject>lead acetate</subject><subject>Lead Poisoning - metabolism</subject><subject>Lead Poisoning - physiopathology</subject><subject>Life Sciences</subject><subject>machinery</subject><subject>Male</subject><subject>muscle strength</subject><subject>Muscles</subject><subject>Myocardial Contraction - drug effects</subject><subject>myocardium</subject><subject>Myocardium - metabolism</subject><subject>Nutrition</subject><subject>Oncology</subject><subject>Organometallic Compounds - toxicity</subject><subject>protein synthesis</subject><subject>Proteins</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Risk factors</subject><subject>Rodents</subject><subject>sarcoplasmic reticulum</subject><subject>Sarcoplasmic Reticulum Calcium-Transporting ATPases - biosynthesis</subject><issn>0163-4984</issn><issn>1559-0720</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kE2LFDEQhoMo7jj6A7xowIuX1spHdSZHGVZdmEVQF7yFmnR6zdLTGZNu1v33ZuhVxIM5JFTlqTfhYey5gDcCwLwtQoIQDQhsQAE08ICtBKJtwEh4yFYgWtVou9Fn7EkpNwDCSKseszOJxmItVuzb-c9jKnMOfEqc-C7d8l2gjm_T6MM4ZZpiGvnF4Ugxl1O3tvwUh8AvyX-PY8h3PI78M018S7mL5PnlXPwQnrJHPQ0lPLs_1-zq_fnX7cdm9-nDxfbdrvGo5NQYgySMwF6AhH2vhO72-2Co2-gehW-V3VCnbeiwD73VCOQVYquwpbZDIrVmr5fcY04_5lAmd4jFh2GgMaS5uBoOEqWwsqKv_kFv0pzH-rtKgUWrjdSVEgvlcyolh94dczxQvnMC3Em7W7S7qt2dtNdtzV7cJ8_7Q-j-TPz2XAG5AKVejdch__X0f1JfLkM9JUfXORZ39aVCLdRldYvqF6bnlJk</recordid><startdate>20151001</startdate><enddate>20151001</enddate><creator>Silva, Marito A. 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S. C</au><au>de Oliveira, Thiago F</au><au>Almenara, Camila C. P</au><au>Broseghini-Filho, Gilson B</au><au>Vassallo, Dalton V</au><au>Padilha, Alessandra S</au><au>Silveira, Edna A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Exposure to a Low Lead Concentration Impairs Contractile Machinery in Rat Cardiac Muscle</atitle><jtitle>Biological trace element research</jtitle><stitle>Biol Trace Elem Res</stitle><addtitle>Biol Trace Elem Res</addtitle><date>2015-10-01</date><risdate>2015</risdate><volume>167</volume><issue>2</issue><spage>280</spage><epage>287</epage><pages>280-287</pages><issn>0163-4984</issn><eissn>1559-0720</eissn><abstract>Lead exposure has been considered to be a risk factor for hypertension and cardiovascular disease. Our purpose was to evaluate the effects of low plasma lead concentration on cardiac contractility in isolated papillary muscles. Wistar rats were divided in control group or group treated with 100 ppm of lead acetate in the drinking water for 15 days. Blood pressure (BP) was measured weekly. At the end of the treatment period, the animals were anesthetized and euthanized, and parameters related to isolated papillary muscle contractility were recorded. The lead concentrations in the blood reached 12.3 ± 2 μg/dL. The BP was increased in the group treated with 100 ppm of lead acetate. Lead treatment did not alter force and time derivatives of the force of left ventricular papillary muscles. In addition, the inotropic response induced by an increase in the extracellular Ca²⁺ concentration was reduced in the Pb²⁺ group. However, the uptake of Ca²⁺ by the sarcoplasmic reticulum and the protein expression of SERCA and phospholamban remained unchanged. Postrest contraction was similar in the both groups, and tetanic peak and plateau tension were reduced in lead group. These results demonstrated that the reduction in the inotropic response to calcium does not appear to be caused by changes in the trans-sarcolemmal calcium flux but suggest that an impairment of the contractile machinery might be taking place. Our results demonstrate that even at a concentration below the limit considered to be safe, lead exerts deleterious effects on the cardiac contractile machinery.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>25795172</pmid><doi>10.1007/s12011-015-0300-0</doi><tpages>8</tpages></addata></record> |
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| subjects | acetates Animals Biochemistry Biomedical and Life Sciences Biotechnology blood Blood pressure Blood Pressure - drug effects Calcium Calcium-Binding Proteins - biosynthesis Cardiomyocytes Cardiovascular diseases Drinking water groups Hypertension Lead lead acetate Lead Poisoning - metabolism Lead Poisoning - physiopathology Life Sciences machinery Male muscle strength Muscles Myocardial Contraction - drug effects myocardium Myocardium - metabolism Nutrition Oncology Organometallic Compounds - toxicity protein synthesis Proteins Rats Rats, Wistar Risk factors Rodents sarcoplasmic reticulum Sarcoplasmic Reticulum Calcium-Transporting ATPases - biosynthesis |
| title | Exposure to a Low Lead Concentration Impairs Contractile Machinery in Rat Cardiac Muscle |
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