Immune-independent and label-free fluorescent assay for Cystatin C detection based on protein-stabilized Au nanoclusters

Cystatin C (Cys C) is a significant cysteine protease inhibitor in human bodies, and is proposed as a fascinating novel marker of glomerular filtration rate for kidney injury detection. Almost all traditional methods for Cys C measurement are immunoassays. In this article, we report a simple, immune...

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Veröffentlicht in:	Biosensors & bioelectronics 2013-03, Vol.41, p.256-261
Hauptverfasser:	Lin, Hui, Li, Lijun, Lei, Chunyang, Xu, Xiahong, Nie, Zhou, Guo, Manli, Huang, Yan, Yao, Shouzhuo
Format:	Artikel
Sprache:	eng
Schlagworte:	absorbance Au nanoclusters Biological and medical sciences Biosensing Techniques - instrumentation biosensors Biotechnology Cystatin C Cystatin C - analysis Cystatin C - chemistry cysteine proteinase inhibitors detection limit enzyme activity Equipment Design Equipment Failure Analysis fluorescence Fluorescence probe Fundamental and applied biological sciences. Psychology glomerular filtration rate gold Gold - chemistry humans Immunoassay - instrumentation immunoassays kidneys light scattering Metal Nanoparticles - chemistry nanoparticles Nanotechnology - instrumentation Papain polyacrylamide gel electrophoresis Protein Interaction Mapping - instrumentation Reproducibility of Results Sensitivity and Specificity Serum Albumin, Bovine - chemistry Spectrometry, Fluorescence - instrumentation Staining and Labeling transmission electron microscopes urine X-ray photoelectron spectroscopy
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