An optimized screening method for identifying levels of resistance to Crinipellis perniciosa in cocoa (Theobroma cacao)
The effects of host age, leaf number, host type (clone or seedling), pathogen spore concentration and incubation time on inoculation with Crinipellis perniciosa (witches’ broom disease of cocoa) were studied in greenhouse experiments using susceptible cocoa genotypes. Three methods of inoculation (a...
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| Veröffentlicht in: | Plant pathology 2003-08, Vol.52 (4), p.464-475 |
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| creator | Surujdeo‐Maharaj, S. Umaharan, P. Butler, D. R. Sreenivasan, T. N. |
| description | The effects of host age, leaf number, host type (clone or seedling), pathogen spore concentration and incubation time on inoculation with Crinipellis perniciosa (witches’ broom disease of cocoa) were studied in greenhouse experiments using susceptible cocoa genotypes. Three methods of inoculation (agar‐drop, water‐drop and spray) were also tested. An optimized inoculation method was selected and tested for its repeatability as well as its ability to discriminate between various levels of resistance to C. perniciosa in cocoa. The optimized method (350 000 viable basidiospores per mL, 60 h incubation, agar‐drop technique) produced 100% infection repeatedly, on both clonal and seedling plants of a susceptible genotype. Seedling age (2–12 months) and leaf number did not significantly affect the percentage of plants with symptoms or broom characteristics. This method discriminated effectively between the various levels of resistance in 14 cocoa genotypes and is recommended as an inoculation method to identify levels of resistance in germplasm collections. Symptom severity was shown to be a better measure of resistance than infection success. |
| doi_str_mv | 10.1046/j.1365-3059.2003.00865.x |
| format | Article |
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Seedling age (2–12 months) and leaf number did not significantly affect the percentage of plants with symptoms or broom characteristics. This method discriminated effectively between the various levels of resistance in 14 cocoa genotypes and is recommended as an inoculation method to identify levels of resistance in germplasm collections. Symptom severity was shown to be a better measure of resistance than infection success.</description><identifier>ISSN: 0032-0862</identifier><identifier>EISSN: 1365-3059</identifier><identifier>DOI: 10.1046/j.1365-3059.2003.00865.x</identifier><identifier>CODEN: PLPAAD</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Biological and medical sciences ; Crinipellis perniciosa ; disease resistance ; Fundamental and applied biological sciences. Psychology ; inoculation method ; Phytopathology. Animal pests. 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R.</creatorcontrib><creatorcontrib>Sreenivasan, T. N.</creatorcontrib><title>An optimized screening method for identifying levels of resistance to Crinipellis perniciosa in cocoa (Theobroma cacao)</title><title>Plant pathology</title><description>The effects of host age, leaf number, host type (clone or seedling), pathogen spore concentration and incubation time on inoculation with Crinipellis perniciosa (witches’ broom disease of cocoa) were studied in greenhouse experiments using susceptible cocoa genotypes. Three methods of inoculation (agar‐drop, water‐drop and spray) were also tested. An optimized inoculation method was selected and tested for its repeatability as well as its ability to discriminate between various levels of resistance to C. perniciosa in cocoa. The optimized method (350 000 viable basidiospores per mL, 60 h incubation, agar‐drop technique) produced 100% infection repeatedly, on both clonal and seedling plants of a susceptible genotype. Seedling age (2–12 months) and leaf number did not significantly affect the percentage of plants with symptoms or broom characteristics. This method discriminated effectively between the various levels of resistance in 14 cocoa genotypes and is recommended as an inoculation method to identify levels of resistance in germplasm collections. Symptom severity was shown to be a better measure of resistance than infection success.</description><subject>Biological and medical sciences</subject><subject>Crinipellis perniciosa</subject><subject>disease resistance</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>inoculation method</subject><subject>Phytopathology. Animal pests. 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N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An optimized screening method for identifying levels of resistance to Crinipellis perniciosa in cocoa (Theobroma cacao)</atitle><jtitle>Plant pathology</jtitle><date>2003-08</date><risdate>2003</risdate><volume>52</volume><issue>4</issue><spage>464</spage><epage>475</epage><pages>464-475</pages><issn>0032-0862</issn><eissn>1365-3059</eissn><coden>PLPAAD</coden><abstract>The effects of host age, leaf number, host type (clone or seedling), pathogen spore concentration and incubation time on inoculation with Crinipellis perniciosa (witches’ broom disease of cocoa) were studied in greenhouse experiments using susceptible cocoa genotypes. Three methods of inoculation (agar‐drop, water‐drop and spray) were also tested. An optimized inoculation method was selected and tested for its repeatability as well as its ability to discriminate between various levels of resistance to C. perniciosa in cocoa. The optimized method (350 000 viable basidiospores per mL, 60 h incubation, agar‐drop technique) produced 100% infection repeatedly, on both clonal and seedling plants of a susceptible genotype. Seedling age (2–12 months) and leaf number did not significantly affect the percentage of plants with symptoms or broom characteristics. This method discriminated effectively between the various levels of resistance in 14 cocoa genotypes and is recommended as an inoculation method to identify levels of resistance in germplasm collections. Symptom severity was shown to be a better measure of resistance than infection success.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><doi>10.1046/j.1365-3059.2003.00865.x</doi><tpages>12</tpages></addata></record> |
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| source | Wiley Free Content; IngentaConnect Free/Open Access Journals; Wiley Online Library Journals Frontfile Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Biological and medical sciences Crinipellis perniciosa disease resistance Fundamental and applied biological sciences. Psychology inoculation method Phytopathology. Animal pests. Plant and forest protection resistance screening Theobroma cacao witches’ broom disease |
| title | An optimized screening method for identifying levels of resistance to Crinipellis perniciosa in cocoa (Theobroma cacao) |
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