Effect of nitrogen-starvation, light intensity and iron on triacylglyceride/carbohydrate production and fatty acid profile of Neochloris oleoabundans HK-129 by a two-stage process
[Display omitted] •A two-staged culture process was used for enhancing TAG/carbohydrate production.•N-starvation and illumination significantly affect TAG/carbohydrate accumulation.•Iron had no marked influence in TAG/carbohydrate accumulation under N-starvation.•The two-stage strategy could obvious...
Gespeichert in:
| Veröffentlicht in: | Bioresource technology 2014-03, Vol.155, p.204-212 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 212 |
|---|---|
| container_issue | |
| container_start_page | 204 |
| container_title | Bioresource technology |
| container_volume | 155 |
| creator | Sun, Xian Cao, Yu Xu, Hui Liu, Yan Sun, Jianrui Qiao, Dairong Cao, Yi |
| description | [Display omitted]
•A two-staged culture process was used for enhancing TAG/carbohydrate production.•N-starvation and illumination significantly affect TAG/carbohydrate accumulation.•Iron had no marked influence in TAG/carbohydrate accumulation under N-starvation.•The two-stage strategy could obviously enhance lipid/carbohydrate productivity.
Triacylglyceride (TAG) and carbohydrate are potential feedstock for biofuels production. In this study, a two-stage process was applied for enhancing TAG/carbohydrate production in the selected microalgae – Neochloris oleoabundans HK-129. In stage I, effects of nitrogen, light intensity and iron on cell growth were investigated, and the highest biomass productivity of 292.83±5.83mg/L/d was achieved. In stage II, different nitrogen-starvation periods, light intensities and iron concentrations were employed to trigger accumulation of TAG and carbohydrate. The culture under 2-day N-starvation, 200μmol/m2/s light intensity and 0.037mM Fe3+ concentration produced the maximum TAG and carbohydrate productivity of 51.58mg/L/d and 90.70mg/L/d, respectively. Nitrogen starvation period and light intensity had marked effects on TAG/carbohydrate accumulation and fatty acids profile, compared to iron concentration. The microalgal lipid was mainly composed of C16/C18 fatty acids (90.02%), saturated fatty acids (29.82%), and monounsaturated fatty acids (32.67%), which is suitable for biodiesel synthesis. |
| doi_str_mv | 10.1016/j.biortech.2013.12.109 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1709793882</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0960852413019494</els_id><sourcerecordid>1709793882</sourcerecordid><originalsourceid>FETCH-LOGICAL-c530t-f134729a246c03ab415a95c4fe8e4a90530d091416bedf5a2de4483fb46555fa3</originalsourceid><addsrcrecordid>eNqNkt9uFCEYxSdGY7fVV2i4MfHC2fJ3ZrjTNLU1Nnqj14SBj102s1CBbbPP5QvKdLd6WRMSEvid853AaZpzgpcEk-5isxx9TAXMekkxYUtC67l80SzI0LOWyr572Syw7HA7CMpPmtOcNxhjRnr6ujmhnIueYbFofl85B6ag6FDwJcUVhDYXne518TF8QJNfrQvyoUDIvuyRDhb5FAOqqySvzX5aTXsDyVu4MDqNcb23SRdAdynanZldHkVOl1luvJ1vnJ9gnvkNollPMfmM4gRRj7tgdcjo5mtLqERjVaDyEOdIq0dLAzm_aV45PWV4e9zPmp-fr35c3rS336-_XH66bY1guLSOMN5TqSnvDGZ65ERoKQx3MADXElfIYkk46UawTmhqgfOBuZF3Qgin2Vnz_uBb5_7aQS5q67OBadIB4i4r0mPZSzYM9HlUsGotpfgflAyUsxqrot0BNSnmnMCpu-S3Ou0VwWqugdqopxqouQaK0Houq_D8OGM3bsH-lT39ewXeHQGdjZ5c0sH4_I-rWXk_zEYfDxzUd773kFQ2HoIB61OtjbLRP5flD0kr1sY</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1518243416</pqid></control><display><type>article</type><title>Effect of nitrogen-starvation, light intensity and iron on triacylglyceride/carbohydrate production and fatty acid profile of Neochloris oleoabundans HK-129 by a two-stage process</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Sun, Xian ; Cao, Yu ; Xu, Hui ; Liu, Yan ; Sun, Jianrui ; Qiao, Dairong ; Cao, Yi</creator><creatorcontrib>Sun, Xian ; Cao, Yu ; Xu, Hui ; Liu, Yan ; Sun, Jianrui ; Qiao, Dairong ; Cao, Yi</creatorcontrib><description>[Display omitted]
•A two-staged culture process was used for enhancing TAG/carbohydrate production.•N-starvation and illumination significantly affect TAG/carbohydrate accumulation.•Iron had no marked influence in TAG/carbohydrate accumulation under N-starvation.•The two-stage strategy could obviously enhance lipid/carbohydrate productivity.
Triacylglyceride (TAG) and carbohydrate are potential feedstock for biofuels production. In this study, a two-stage process was applied for enhancing TAG/carbohydrate production in the selected microalgae – Neochloris oleoabundans HK-129. In stage I, effects of nitrogen, light intensity and iron on cell growth were investigated, and the highest biomass productivity of 292.83±5.83mg/L/d was achieved. In stage II, different nitrogen-starvation periods, light intensities and iron concentrations were employed to trigger accumulation of TAG and carbohydrate. The culture under 2-day N-starvation, 200μmol/m2/s light intensity and 0.037mM Fe3+ concentration produced the maximum TAG and carbohydrate productivity of 51.58mg/L/d and 90.70mg/L/d, respectively. Nitrogen starvation period and light intensity had marked effects on TAG/carbohydrate accumulation and fatty acids profile, compared to iron concentration. The microalgal lipid was mainly composed of C16/C18 fatty acids (90.02%), saturated fatty acids (29.82%), and monounsaturated fatty acids (32.67%), which is suitable for biodiesel synthesis.</description><identifier>ISSN: 0960-8524</identifier><identifier>EISSN: 1873-2976</identifier><identifier>DOI: 10.1016/j.biortech.2013.12.109</identifier><identifier>PMID: 24457305</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Analysis of Variance ; Biodiesel ; Biological and medical sciences ; Carbohydrate ; Carbohydrates ; Carbohydrates - biosynthesis ; Cell Culture Techniques ; Chlorophyta - drug effects ; Chlorophyta - metabolism ; Chlorophyta - radiation effects ; Culture ; Fatty acids ; Fatty Acids - metabolism ; Feedstock ; Fundamental and applied biological sciences. Psychology ; Industrial Microbiology - methods ; Iron ; Iron - pharmacology ; Light ; Lipids ; Microalgae ; Neochloris oleoabundans ; Nitrogen - deficiency ; Nitrogen starvation ; Oxazines ; Productivity ; Triacylglycerol ; Triglycerides - biosynthesis ; Two-stage process</subject><ispartof>Bioresource technology, 2014-03, Vol.155, p.204-212</ispartof><rights>2014 Elsevier Ltd</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2014 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c530t-f134729a246c03ab415a95c4fe8e4a90530d091416bedf5a2de4483fb46555fa3</citedby><cites>FETCH-LOGICAL-c530t-f134729a246c03ab415a95c4fe8e4a90530d091416bedf5a2de4483fb46555fa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0960852413019494$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=28394789$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24457305$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sun, Xian</creatorcontrib><creatorcontrib>Cao, Yu</creatorcontrib><creatorcontrib>Xu, Hui</creatorcontrib><creatorcontrib>Liu, Yan</creatorcontrib><creatorcontrib>Sun, Jianrui</creatorcontrib><creatorcontrib>Qiao, Dairong</creatorcontrib><creatorcontrib>Cao, Yi</creatorcontrib><title>Effect of nitrogen-starvation, light intensity and iron on triacylglyceride/carbohydrate production and fatty acid profile of Neochloris oleoabundans HK-129 by a two-stage process</title><title>Bioresource technology</title><addtitle>Bioresour Technol</addtitle><description>[Display omitted]
•A two-staged culture process was used for enhancing TAG/carbohydrate production.•N-starvation and illumination significantly affect TAG/carbohydrate accumulation.•Iron had no marked influence in TAG/carbohydrate accumulation under N-starvation.•The two-stage strategy could obviously enhance lipid/carbohydrate productivity.
Triacylglyceride (TAG) and carbohydrate are potential feedstock for biofuels production. In this study, a two-stage process was applied for enhancing TAG/carbohydrate production in the selected microalgae – Neochloris oleoabundans HK-129. In stage I, effects of nitrogen, light intensity and iron on cell growth were investigated, and the highest biomass productivity of 292.83±5.83mg/L/d was achieved. In stage II, different nitrogen-starvation periods, light intensities and iron concentrations were employed to trigger accumulation of TAG and carbohydrate. The culture under 2-day N-starvation, 200μmol/m2/s light intensity and 0.037mM Fe3+ concentration produced the maximum TAG and carbohydrate productivity of 51.58mg/L/d and 90.70mg/L/d, respectively. Nitrogen starvation period and light intensity had marked effects on TAG/carbohydrate accumulation and fatty acids profile, compared to iron concentration. The microalgal lipid was mainly composed of C16/C18 fatty acids (90.02%), saturated fatty acids (29.82%), and monounsaturated fatty acids (32.67%), which is suitable for biodiesel synthesis.</description><subject>Analysis of Variance</subject><subject>Biodiesel</subject><subject>Biological and medical sciences</subject><subject>Carbohydrate</subject><subject>Carbohydrates</subject><subject>Carbohydrates - biosynthesis</subject><subject>Cell Culture Techniques</subject><subject>Chlorophyta - drug effects</subject><subject>Chlorophyta - metabolism</subject><subject>Chlorophyta - radiation effects</subject><subject>Culture</subject><subject>Fatty acids</subject><subject>Fatty Acids - metabolism</subject><subject>Feedstock</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Industrial Microbiology - methods</subject><subject>Iron</subject><subject>Iron - pharmacology</subject><subject>Light</subject><subject>Lipids</subject><subject>Microalgae</subject><subject>Neochloris oleoabundans</subject><subject>Nitrogen - deficiency</subject><subject>Nitrogen starvation</subject><subject>Oxazines</subject><subject>Productivity</subject><subject>Triacylglycerol</subject><subject>Triglycerides - biosynthesis</subject><subject>Two-stage process</subject><issn>0960-8524</issn><issn>1873-2976</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkt9uFCEYxSdGY7fVV2i4MfHC2fJ3ZrjTNLU1Nnqj14SBj102s1CBbbPP5QvKdLd6WRMSEvid853AaZpzgpcEk-5isxx9TAXMekkxYUtC67l80SzI0LOWyr572Syw7HA7CMpPmtOcNxhjRnr6ujmhnIueYbFofl85B6ag6FDwJcUVhDYXne518TF8QJNfrQvyoUDIvuyRDhb5FAOqqySvzX5aTXsDyVu4MDqNcb23SRdAdynanZldHkVOl1luvJ1vnJ9gnvkNollPMfmM4gRRj7tgdcjo5mtLqERjVaDyEOdIq0dLAzm_aV45PWV4e9zPmp-fr35c3rS336-_XH66bY1guLSOMN5TqSnvDGZ65ERoKQx3MADXElfIYkk46UawTmhqgfOBuZF3Qgin2Vnz_uBb5_7aQS5q67OBadIB4i4r0mPZSzYM9HlUsGotpfgflAyUsxqrot0BNSnmnMCpu-S3Ou0VwWqugdqopxqouQaK0Houq_D8OGM3bsH-lT39ewXeHQGdjZ5c0sH4_I-rWXk_zEYfDxzUd773kFQ2HoIB61OtjbLRP5flD0kr1sY</recordid><startdate>20140301</startdate><enddate>20140301</enddate><creator>Sun, Xian</creator><creator>Cao, Yu</creator><creator>Xu, Hui</creator><creator>Liu, Yan</creator><creator>Sun, Jianrui</creator><creator>Qiao, Dairong</creator><creator>Cao, Yi</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>7ST</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H98</scope><scope>L.G</scope><scope>P64</scope><scope>SOI</scope><scope>7SU</scope><scope>7TB</scope><scope>KR7</scope></search><sort><creationdate>20140301</creationdate><title>Effect of nitrogen-starvation, light intensity and iron on triacylglyceride/carbohydrate production and fatty acid profile of Neochloris oleoabundans HK-129 by a two-stage process</title><author>Sun, Xian ; Cao, Yu ; Xu, Hui ; Liu, Yan ; Sun, Jianrui ; Qiao, Dairong ; Cao, Yi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c530t-f134729a246c03ab415a95c4fe8e4a90530d091416bedf5a2de4483fb46555fa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Analysis of Variance</topic><topic>Biodiesel</topic><topic>Biological and medical sciences</topic><topic>Carbohydrate</topic><topic>Carbohydrates</topic><topic>Carbohydrates - biosynthesis</topic><topic>Cell Culture Techniques</topic><topic>Chlorophyta - drug effects</topic><topic>Chlorophyta - metabolism</topic><topic>Chlorophyta - radiation effects</topic><topic>Culture</topic><topic>Fatty acids</topic><topic>Fatty Acids - metabolism</topic><topic>Feedstock</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Industrial Microbiology - methods</topic><topic>Iron</topic><topic>Iron - pharmacology</topic><topic>Light</topic><topic>Lipids</topic><topic>Microalgae</topic><topic>Neochloris oleoabundans</topic><topic>Nitrogen - deficiency</topic><topic>Nitrogen starvation</topic><topic>Oxazines</topic><topic>Productivity</topic><topic>Triacylglycerol</topic><topic>Triglycerides - biosynthesis</topic><topic>Two-stage process</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sun, Xian</creatorcontrib><creatorcontrib>Cao, Yu</creatorcontrib><creatorcontrib>Xu, Hui</creatorcontrib><creatorcontrib>Liu, Yan</creatorcontrib><creatorcontrib>Sun, Jianrui</creatorcontrib><creatorcontrib>Qiao, Dairong</creatorcontrib><creatorcontrib>Cao, Yi</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Environment Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><collection>Environmental Engineering Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Civil Engineering Abstracts</collection><jtitle>Bioresource technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sun, Xian</au><au>Cao, Yu</au><au>Xu, Hui</au><au>Liu, Yan</au><au>Sun, Jianrui</au><au>Qiao, Dairong</au><au>Cao, Yi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of nitrogen-starvation, light intensity and iron on triacylglyceride/carbohydrate production and fatty acid profile of Neochloris oleoabundans HK-129 by a two-stage process</atitle><jtitle>Bioresource technology</jtitle><addtitle>Bioresour Technol</addtitle><date>2014-03-01</date><risdate>2014</risdate><volume>155</volume><spage>204</spage><epage>212</epage><pages>204-212</pages><issn>0960-8524</issn><eissn>1873-2976</eissn><abstract>[Display omitted]
•A two-staged culture process was used for enhancing TAG/carbohydrate production.•N-starvation and illumination significantly affect TAG/carbohydrate accumulation.•Iron had no marked influence in TAG/carbohydrate accumulation under N-starvation.•The two-stage strategy could obviously enhance lipid/carbohydrate productivity.
Triacylglyceride (TAG) and carbohydrate are potential feedstock for biofuels production. In this study, a two-stage process was applied for enhancing TAG/carbohydrate production in the selected microalgae – Neochloris oleoabundans HK-129. In stage I, effects of nitrogen, light intensity and iron on cell growth were investigated, and the highest biomass productivity of 292.83±5.83mg/L/d was achieved. In stage II, different nitrogen-starvation periods, light intensities and iron concentrations were employed to trigger accumulation of TAG and carbohydrate. The culture under 2-day N-starvation, 200μmol/m2/s light intensity and 0.037mM Fe3+ concentration produced the maximum TAG and carbohydrate productivity of 51.58mg/L/d and 90.70mg/L/d, respectively. Nitrogen starvation period and light intensity had marked effects on TAG/carbohydrate accumulation and fatty acids profile, compared to iron concentration. The microalgal lipid was mainly composed of C16/C18 fatty acids (90.02%), saturated fatty acids (29.82%), and monounsaturated fatty acids (32.67%), which is suitable for biodiesel synthesis.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>24457305</pmid><doi>10.1016/j.biortech.2013.12.109</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0960-8524 |
| ispartof | Bioresource technology, 2014-03, Vol.155, p.204-212 |
| issn | 0960-8524 1873-2976 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1709793882 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Analysis of Variance Biodiesel Biological and medical sciences Carbohydrate Carbohydrates Carbohydrates - biosynthesis Cell Culture Techniques Chlorophyta - drug effects Chlorophyta - metabolism Chlorophyta - radiation effects Culture Fatty acids Fatty Acids - metabolism Feedstock Fundamental and applied biological sciences. Psychology Industrial Microbiology - methods Iron Iron - pharmacology Light Lipids Microalgae Neochloris oleoabundans Nitrogen - deficiency Nitrogen starvation Oxazines Productivity Triacylglycerol Triglycerides - biosynthesis Two-stage process |
| title | Effect of nitrogen-starvation, light intensity and iron on triacylglyceride/carbohydrate production and fatty acid profile of Neochloris oleoabundans HK-129 by a two-stage process |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-02T19%3A12%3A18IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effect%20of%20nitrogen-starvation,%20light%20intensity%20and%20iron%20on%20triacylglyceride/carbohydrate%20production%20and%20fatty%20acid%20profile%20of%20Neochloris%20oleoabundans%20HK-129%20by%20a%20two-stage%20process&rft.jtitle=Bioresource%20technology&rft.au=Sun,%20Xian&rft.date=2014-03-01&rft.volume=155&rft.spage=204&rft.epage=212&rft.pages=204-212&rft.issn=0960-8524&rft.eissn=1873-2976&rft_id=info:doi/10.1016/j.biortech.2013.12.109&rft_dat=%3Cproquest_cross%3E1709793882%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1518243416&rft_id=info:pmid/24457305&rft_els_id=S0960852413019494&rfr_iscdi=true |