A multiple-labelling method for cells using Au nanoparticles with different shapes

An important and difficult issue is simultaneously identifying the detailed locations of various molecules on the cell surface, as this identification requires a synergistic effect between more than one molecule in a living cell. Au nanoparticles (NPs) with different shapes can be readily recognised...

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Veröffentlicht in:Chinese science bulletin 2013-07, Vol.58 (21), p.2640-2645
Hauptverfasser: Zhang, Ke, Feng, JianTao, Sun, QuanMei, Jin, Lin, Li, Jing, Wu, XiaoChun, Han, Dong
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container_end_page 2645
container_issue 21
container_start_page 2640
container_title Chinese science bulletin
container_volume 58
creator Zhang, Ke
Feng, JianTao
Sun, QuanMei
Jin, Lin
Li, Jing
Wu, XiaoChun
Han, Dong
description An important and difficult issue is simultaneously identifying the detailed locations of various molecules on the cell surface, as this identification requires a synergistic effect between more than one molecule in a living cell. Au nanoparticles (NPs) with different shapes can be readily recognised under low vacuum scanning electron microscopy (lvSEM). Anisotropic Au nanorods (NRs) possess unique surface plasmon resonance (SPR) properties, which can be further utilised for two photon luminescence (TPL) and other optical imaging techniques. In this paper, Au NRs and Au nanooctahedra (Au NOs) are introduced as biomarkers for ICAM-1 and Integrin β1. Combined with the advantages of lvSEM, this multiple-labelling method is a new method for studying the interactions between specific, functional molecules.
doi_str_mv 10.1007/s11434-013-5794-2
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source EZB-FREE-00999 freely available EZB journals
subjects Biomarkers
Chemistry/Food Science
Earth Sciences
Engineering
Gold
Humanities and Social Sciences
ICAM-1
integrins
Life Sciences
Luminescence
multidisciplinary
Nanocomposites
Nanomaterials
Nanoparticles
Nanorods
Nanostructure
new methods
Physics
scanning electron microscopy
Science
Science (multidisciplinary)
surface plasmon resonance
synergism
形状
扫描电子显微镜
标签
细胞表面
自然保护区
表面等离激元
金纳米粒子
title A multiple-labelling method for cells using Au nanoparticles with different shapes
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